Cytokinin modulates the inhibitory effect of shade stress on photosynthesis, antioxidant capacity and hormone homeostasis to regulate the grain yield in wheat

Agroforestry intercropping is an effective way to optimize land use and ensure food security. However, the physiological mechanism by which the shading of dominant plants inhibits the yield of non-dominant plants in this mode remains to be investigated. A two-year location experiment of walnut-winte...

Full description

Saved in:
Bibliographic Details
Main Authors: Yongqiang Zhang, Juan Li, Qijiang Xu, Chuanxin Chen, Shihui Nie, Junjie Lei, Liusheng Duan
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-11-01
Series:Frontiers in Plant Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2024.1498123/full
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850146659100524544
author Yongqiang Zhang
Yongqiang Zhang
Yongqiang Zhang
Juan Li
Juan Li
Qijiang Xu
Qijiang Xu
Chuanxin Chen
Chuanxin Chen
Shihui Nie
Shihui Nie
Junjie Lei
Junjie Lei
Liusheng Duan
author_facet Yongqiang Zhang
Yongqiang Zhang
Yongqiang Zhang
Juan Li
Juan Li
Qijiang Xu
Qijiang Xu
Chuanxin Chen
Chuanxin Chen
Shihui Nie
Shihui Nie
Junjie Lei
Junjie Lei
Liusheng Duan
author_sort Yongqiang Zhang
collection DOAJ
description Agroforestry intercropping is an effective way to optimize land use and ensure food security. However, the physiological mechanism by which the shading of dominant plants inhibits the yield of non-dominant plants in this mode remains to be investigated. A two-year location experiment of walnut-winter wheat intercrop combined with exogenous 6-benzyladenine (6-BA, the first synthetic cytokinin) treatment was conducted to reveal the mechanism of 6-BA in inhibiting wheat growth and yield formation under shade stress by measuring the photosynthetic characteristics, antioxidant capacity, hormone homeostasis of wheat flag leaves and yield. The results showed that compared with far canopy area (FCA), antioxidant enzyme activity [e.g. superoxide dismutase (SOD), peroxidase (POD)], zeatin (ZT) and abscisic acid (ABA) content and photosynthesis of wheat flag leaves were significantly reduced in below canopy area (BCA) treatment during flowering and grain filling stages, thereby inhibiting wheat dry matter accumulation and yield formation. Exogenous 6-BA significantly increased hormone [i.e. indoleacetic acid (IAA), ZT and gibberellin (GA)] levels, antioxidant enzyme activities and photosynthesis in flag leaves, thereby increasing dry matter and yield, especially in the FCA condition. Furthermore, net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), activities of ribulose bisphosphate carboxylase (RuBPCase) and phosphoenolpyruvate carboxylase (PEPCase), ABA and ZT concentrations of flag leaves at flowering and filling stages had a significant contribution to yield formation under 6-BA and shade treatments. Overall, cytokinin regulates the inhibitory effects of shade stress on wheat photosynthesis, antioxidant capacity and hormone homeostasis to reduce wheat yield loss.
format Article
id doaj-art-2a31e566f712484bb8c0933346d5d7b7
institution OA Journals
issn 1664-462X
language English
publishDate 2024-11-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Plant Science
spelling doaj-art-2a31e566f712484bb8c0933346d5d7b72025-08-20T02:27:46ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-11-011510.3389/fpls.2024.14981231498123Cytokinin modulates the inhibitory effect of shade stress on photosynthesis, antioxidant capacity and hormone homeostasis to regulate the grain yield in wheatYongqiang Zhang0Yongqiang Zhang1Yongqiang Zhang2Juan Li3Juan Li4Qijiang Xu5Qijiang Xu6Chuanxin Chen7Chuanxin Chen8Shihui Nie9Shihui Nie10Junjie Lei11Junjie Lei12Liusheng Duan13College of Agronomy and Biotechnology, China Agricultural University, Beijing, ChinaResearch Institute of Grain Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, ChinaKey Laboratory of Desert-Oasis Crop Physiology, Ecology and Cultivation, Urumqi, Xinjiang, ChinaResearch Institute of Grain Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, ChinaKey Laboratory of Desert-Oasis Crop Physiology, Ecology and Cultivation, Urumqi, Xinjiang, ChinaResearch Institute of Grain Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, ChinaKey Laboratory of Desert-Oasis Crop Physiology, Ecology and Cultivation, Urumqi, Xinjiang, ChinaResearch Institute of Grain Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, ChinaKey Laboratory of Desert-Oasis Crop Physiology, Ecology and Cultivation, Urumqi, Xinjiang, ChinaResearch Institute of Grain Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, ChinaKey Laboratory of Desert-Oasis Crop Physiology, Ecology and Cultivation, Urumqi, Xinjiang, ChinaResearch Institute of Grain Crops, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, ChinaKey Laboratory of Desert-Oasis Crop Physiology, Ecology and Cultivation, Urumqi, Xinjiang, ChinaCollege of Agronomy and Biotechnology, China Agricultural University, Beijing, ChinaAgroforestry intercropping is an effective way to optimize land use and ensure food security. However, the physiological mechanism by which the shading of dominant plants inhibits the yield of non-dominant plants in this mode remains to be investigated. A two-year location experiment of walnut-winter wheat intercrop combined with exogenous 6-benzyladenine (6-BA, the first synthetic cytokinin) treatment was conducted to reveal the mechanism of 6-BA in inhibiting wheat growth and yield formation under shade stress by measuring the photosynthetic characteristics, antioxidant capacity, hormone homeostasis of wheat flag leaves and yield. The results showed that compared with far canopy area (FCA), antioxidant enzyme activity [e.g. superoxide dismutase (SOD), peroxidase (POD)], zeatin (ZT) and abscisic acid (ABA) content and photosynthesis of wheat flag leaves were significantly reduced in below canopy area (BCA) treatment during flowering and grain filling stages, thereby inhibiting wheat dry matter accumulation and yield formation. Exogenous 6-BA significantly increased hormone [i.e. indoleacetic acid (IAA), ZT and gibberellin (GA)] levels, antioxidant enzyme activities and photosynthesis in flag leaves, thereby increasing dry matter and yield, especially in the FCA condition. Furthermore, net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci), activities of ribulose bisphosphate carboxylase (RuBPCase) and phosphoenolpyruvate carboxylase (PEPCase), ABA and ZT concentrations of flag leaves at flowering and filling stages had a significant contribution to yield formation under 6-BA and shade treatments. Overall, cytokinin regulates the inhibitory effects of shade stress on wheat photosynthesis, antioxidant capacity and hormone homeostasis to reduce wheat yield loss.https://www.frontiersin.org/articles/10.3389/fpls.2024.1498123/fullwalnut-wheat intercropping6-benzyladeninehormonewheat yieldphysiological mechanism
spellingShingle Yongqiang Zhang
Yongqiang Zhang
Yongqiang Zhang
Juan Li
Juan Li
Qijiang Xu
Qijiang Xu
Chuanxin Chen
Chuanxin Chen
Shihui Nie
Shihui Nie
Junjie Lei
Junjie Lei
Liusheng Duan
Cytokinin modulates the inhibitory effect of shade stress on photosynthesis, antioxidant capacity and hormone homeostasis to regulate the grain yield in wheat
Frontiers in Plant Science
walnut-wheat intercropping
6-benzyladenine
hormone
wheat yield
physiological mechanism
title Cytokinin modulates the inhibitory effect of shade stress on photosynthesis, antioxidant capacity and hormone homeostasis to regulate the grain yield in wheat
title_full Cytokinin modulates the inhibitory effect of shade stress on photosynthesis, antioxidant capacity and hormone homeostasis to regulate the grain yield in wheat
title_fullStr Cytokinin modulates the inhibitory effect of shade stress on photosynthesis, antioxidant capacity and hormone homeostasis to regulate the grain yield in wheat
title_full_unstemmed Cytokinin modulates the inhibitory effect of shade stress on photosynthesis, antioxidant capacity and hormone homeostasis to regulate the grain yield in wheat
title_short Cytokinin modulates the inhibitory effect of shade stress on photosynthesis, antioxidant capacity and hormone homeostasis to regulate the grain yield in wheat
title_sort cytokinin modulates the inhibitory effect of shade stress on photosynthesis antioxidant capacity and hormone homeostasis to regulate the grain yield in wheat
topic walnut-wheat intercropping
6-benzyladenine
hormone
wheat yield
physiological mechanism
url https://www.frontiersin.org/articles/10.3389/fpls.2024.1498123/full
work_keys_str_mv AT yongqiangzhang cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat
AT yongqiangzhang cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat
AT yongqiangzhang cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat
AT juanli cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat
AT juanli cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat
AT qijiangxu cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat
AT qijiangxu cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat
AT chuanxinchen cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat
AT chuanxinchen cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat
AT shihuinie cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat
AT shihuinie cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat
AT junjielei cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat
AT junjielei cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat
AT liushengduan cytokininmodulatestheinhibitoryeffectofshadestressonphotosynthesisantioxidantcapacityandhormonehomeostasistoregulatethegrainyieldinwheat