Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single Spawning
<p class="IsiAbstrakIndo"><span lang="EN-GB">Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cyto...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Negeri Semarang
2017-07-01
|
| Series: | Biosaintifika: Journal of Biology & Biology Education |
| Subjects: | |
| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/9301 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850237275423637504 |
|---|---|
| author | Agus Nuryanto Nael Huda Qonita Hendro Pramono Kusbiyanto Kusbiyanto Petrus Hary Tjahja Soedibja |
| author_facet | Agus Nuryanto Nael Huda Qonita Hendro Pramono Kusbiyanto Kusbiyanto Petrus Hary Tjahja Soedibja |
| author_sort | Agus Nuryanto |
| collection | DOAJ |
| description | <p class="IsiAbstrakIndo"><span lang="EN-GB">Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. The result showed that PCR-RFLP markers of Cytochrome b-</span><em><span lang="EN-GB">Hinf</span></em><span lang="EN-GB">I of 315 bp, and 210 bp, and also Cytochrome b-</span><em><span lang="EN-GB">Alu</span></em><span lang="EN-GB">I of 334 bp and 189 bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b-</span><em><span lang="EN-GB"> Hinf</span></em><span lang="EN-GB">I 366 bp, and 159 bp and Cytochrome b-</span><em><span lang="EN-GB">Alu</span></em><span lang="EN-GB">I 525 bp fragments. It is observed that genetic variation of Cytochrome b-</span><em><span lang="EN-GB">Hinf</span></em><span lang="EN-GB">I and -</span><em><span lang="EN-GB">Alu</span></em><span lang="EN-GB">I markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-</span><em><span lang="EN-GB">Hinf</span></em><span lang="EN-GB">I and -</span><em><span lang="EN-GB">Alu</span></em><span lang="EN-GB">I gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries. </span></p> |
| format | Article |
| id | doaj-art-2a2a57314697484a92d3d2c795e6a66a |
| institution | OA Journals |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2017-07-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-2a2a57314697484a92d3d2c795e6a66a2025-08-20T02:01:46ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102017-07-019218519210.15294/biosaintifika.v9i2.93016157Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single SpawningAgus Nuryanto0Nael Huda Qonita1Hendro Pramono2Kusbiyanto Kusbiyanto3Petrus Hary Tjahja Soedibja4Faculty of Biology, Universitas Jenderal Soedirman, IndonesiaFaculty of Biology, Universitas Jenderal Soedirman, IndonesiaFaculty of Biology, Universitas Jenderal Soedirman, IndonesiaFaculty of Biology, Universitas Jenderal Soedirman, IndonesiaFaculty of Fisheries and Marine Sciences, Universitas Jenderal Soedirman, Indonesia<p class="IsiAbstrakIndo"><span lang="EN-GB">Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. The result showed that PCR-RFLP markers of Cytochrome b-</span><em><span lang="EN-GB">Hinf</span></em><span lang="EN-GB">I of 315 bp, and 210 bp, and also Cytochrome b-</span><em><span lang="EN-GB">Alu</span></em><span lang="EN-GB">I of 334 bp and 189 bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b-</span><em><span lang="EN-GB"> Hinf</span></em><span lang="EN-GB">I 366 bp, and 159 bp and Cytochrome b-</span><em><span lang="EN-GB">Alu</span></em><span lang="EN-GB">I 525 bp fragments. It is observed that genetic variation of Cytochrome b-</span><em><span lang="EN-GB">Hinf</span></em><span lang="EN-GB">I and -</span><em><span lang="EN-GB">Alu</span></em><span lang="EN-GB">I markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-</span><em><span lang="EN-GB">Hinf</span></em><span lang="EN-GB">I and -</span><em><span lang="EN-GB">Alu</span></em><span lang="EN-GB">I gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries. </span></p>https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/9301soang strainsingle spawningcytochrome b genegenetic diversityPCR-RFLP markers |
| spellingShingle | Agus Nuryanto Nael Huda Qonita Hendro Pramono Kusbiyanto Kusbiyanto Petrus Hary Tjahja Soedibja Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single Spawning Biosaintifika: Journal of Biology & Biology Education soang strain single spawning cytochrome b gene genetic diversity PCR-RFLP markers |
| title | Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single Spawning |
| title_full | Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single Spawning |
| title_fullStr | Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single Spawning |
| title_full_unstemmed | Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single Spawning |
| title_short | Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single Spawning |
| title_sort | genetic variation in cytochrome b hinf1 and alu1 gene correlated to body size in soang gourami osphronemus goramy from single spawning |
| topic | soang strain single spawning cytochrome b gene genetic diversity PCR-RFLP markers |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/9301 |
| work_keys_str_mv | AT agusnuryanto geneticvariationincytochromebhinf1andalu1genecorrelatedtobodysizeinsoanggouramiosphronemusgoramyfromsinglespawning AT naelhudaqonita geneticvariationincytochromebhinf1andalu1genecorrelatedtobodysizeinsoanggouramiosphronemusgoramyfromsinglespawning AT hendropramono geneticvariationincytochromebhinf1andalu1genecorrelatedtobodysizeinsoanggouramiosphronemusgoramyfromsinglespawning AT kusbiyantokusbiyanto geneticvariationincytochromebhinf1andalu1genecorrelatedtobodysizeinsoanggouramiosphronemusgoramyfromsinglespawning AT petrusharytjahjasoedibja geneticvariationincytochromebhinf1andalu1genecorrelatedtobodysizeinsoanggouramiosphronemusgoramyfromsinglespawning |