Determination of Dyclonine Hydrochloride by a HPLC Method and Camphor and Menthol by a GC Method in Compound Lotion
A high performance liquid chromatographic (HPLC) method with UV detector for the determination of dyclonine hydrochloride and a gas chromatography (GC) method with flame ionization detector (FID) for the determination of camphor and menthol in lotion were developed. The developed HPLC method involve...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/154087 |
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| author | Suying Ma Haixia Lv Xiaojun Shang |
| author_facet | Suying Ma Haixia Lv Xiaojun Shang |
| author_sort | Suying Ma |
| collection | DOAJ |
| description | A high performance liquid chromatographic (HPLC) method with UV detector for the determination of dyclonine hydrochloride and a gas chromatography (GC) method with flame ionization detector (FID) for the determination of camphor and menthol in lotion were developed. The developed HPLC method involved using a SinoChoom ODS-BP C18 reversed-phase column (5 μm, 4.6 mm × 200 mm) and mobile phase consisting of acetonitrile : water : triethylamine in a ratio of 45 : 55 : 1.0; pH was adjusted to 3.5 with glacial acetic acid. The developed GC method for determination of camphor and menthol involved using an Agilent 19091J-413 capillary chromatographic column (30 m × 320 μm × 0.25 μm). The two methods were validated according to official compendia guidelines. The calibration of dyclonine hydrochloride for HPLC method was linear over the range of 20–200 μg/mL. The retention time was found at 6.0 min for dyclonine hydrochloride. The calibration of camphor and menthol of GC method was linear over the range of 10–2000 μg/mL. The retention time was found at 2.9 min for camphor and 3.05 min for menthol. The proposed HPLC and GC methods were proved to be suitable for the determination of dyclonine hydrochloride, camphor, and menthol in lotion. |
| format | Article |
| id | doaj-art-2a22413eb6434839bcda54ef091d5aca |
| institution | OA Journals |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-2a22413eb6434839bcda54ef091d5aca2025-08-20T02:03:59ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/154087154087Determination of Dyclonine Hydrochloride by a HPLC Method and Camphor and Menthol by a GC Method in Compound LotionSuying Ma0Haixia Lv1Xiaojun Shang2School of Pharmacy, Xinxiang Medical University, Xinxiang 453003, ChinaSchool of Pharmacy, Xinxiang Medical University, Xinxiang 453003, ChinaSchool of Pharmacy, Xinxiang Medical University, Xinxiang 453003, ChinaA high performance liquid chromatographic (HPLC) method with UV detector for the determination of dyclonine hydrochloride and a gas chromatography (GC) method with flame ionization detector (FID) for the determination of camphor and menthol in lotion were developed. The developed HPLC method involved using a SinoChoom ODS-BP C18 reversed-phase column (5 μm, 4.6 mm × 200 mm) and mobile phase consisting of acetonitrile : water : triethylamine in a ratio of 45 : 55 : 1.0; pH was adjusted to 3.5 with glacial acetic acid. The developed GC method for determination of camphor and menthol involved using an Agilent 19091J-413 capillary chromatographic column (30 m × 320 μm × 0.25 μm). The two methods were validated according to official compendia guidelines. The calibration of dyclonine hydrochloride for HPLC method was linear over the range of 20–200 μg/mL. The retention time was found at 6.0 min for dyclonine hydrochloride. The calibration of camphor and menthol of GC method was linear over the range of 10–2000 μg/mL. The retention time was found at 2.9 min for camphor and 3.05 min for menthol. The proposed HPLC and GC methods were proved to be suitable for the determination of dyclonine hydrochloride, camphor, and menthol in lotion.http://dx.doi.org/10.1155/2013/154087 |
| spellingShingle | Suying Ma Haixia Lv Xiaojun Shang Determination of Dyclonine Hydrochloride by a HPLC Method and Camphor and Menthol by a GC Method in Compound Lotion Journal of Chemistry |
| title | Determination of Dyclonine Hydrochloride by a HPLC Method and Camphor and Menthol by a GC Method in Compound Lotion |
| title_full | Determination of Dyclonine Hydrochloride by a HPLC Method and Camphor and Menthol by a GC Method in Compound Lotion |
| title_fullStr | Determination of Dyclonine Hydrochloride by a HPLC Method and Camphor and Menthol by a GC Method in Compound Lotion |
| title_full_unstemmed | Determination of Dyclonine Hydrochloride by a HPLC Method and Camphor and Menthol by a GC Method in Compound Lotion |
| title_short | Determination of Dyclonine Hydrochloride by a HPLC Method and Camphor and Menthol by a GC Method in Compound Lotion |
| title_sort | determination of dyclonine hydrochloride by a hplc method and camphor and menthol by a gc method in compound lotion |
| url | http://dx.doi.org/10.1155/2013/154087 |
| work_keys_str_mv | AT suyingma determinationofdycloninehydrochloridebyahplcmethodandcamphorandmentholbyagcmethodincompoundlotion AT haixialv determinationofdycloninehydrochloridebyahplcmethodandcamphorandmentholbyagcmethodincompoundlotion AT xiaojunshang determinationofdycloninehydrochloridebyahplcmethodandcamphorandmentholbyagcmethodincompoundlotion |