Computer‐aided rational design of the phosphotransferase system for enhanced glucose uptake in Escherichia coli
Abstract The phosphotransferase system (PTS) is the sugar transportation machinery that is widely distributed in prokaryotes and is critical for enhanced production of useful metabolites. To increase the glucose uptake rate, we propose a rational strategy for designing the molecular architecture of...
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| Format: | Article |
| Language: | English |
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Springer Nature
2008-01-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.1038/msb4100201 |
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| _version_ | 1849225751977525248 |
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| author | Yousuke Nishio Yoshihiro Usuda Kazuhiko Matsui Hiroyuki Kurata |
| author_facet | Yousuke Nishio Yoshihiro Usuda Kazuhiko Matsui Hiroyuki Kurata |
| author_sort | Yousuke Nishio |
| collection | DOAJ |
| description | Abstract The phosphotransferase system (PTS) is the sugar transportation machinery that is widely distributed in prokaryotes and is critical for enhanced production of useful metabolites. To increase the glucose uptake rate, we propose a rational strategy for designing the molecular architecture of the Escherichia coli glucose PTS by using a computer‐aided design (CAD) system and verified the simulated results with biological experiments. CAD supports construction of a biochemical map, mathematical modeling, simulation, and system analysis. Assuming that the PTS aims at controlling the glucose uptake rate, the PTS was decomposed into hierarchical modules, functional and flux modules, and the effect of changes in gene expression on the glucose uptake rate was simulated to make a rational strategy of how the gene regulatory network is engineered. Such design and analysis predicted that the mlc knockout mutant with ptsI gene overexpression would greatly increase the specific glucose uptake rate. By using biological experiments, we validated the prediction and the presented strategy, thereby enhancing the specific glucose uptake rate. |
| format | Article |
| id | doaj-art-2a1571b227b94064b8ada5fe00bfcc9c |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-2a1571b227b94064b8ada5fe00bfcc9c2025-08-24T12:01:40ZengSpringer NatureMolecular Systems Biology1744-42922008-01-014111210.1038/msb4100201Computer‐aided rational design of the phosphotransferase system for enhanced glucose uptake in Escherichia coliYousuke Nishio0Yoshihiro Usuda1Kazuhiko Matsui2Hiroyuki Kurata3Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc.Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc.Fermentation and Biotechnology Laboratories, Ajinomoto Co. Inc.Department of Bioscience and Bioinformatics, Kyushu Institute of TechnologyAbstract The phosphotransferase system (PTS) is the sugar transportation machinery that is widely distributed in prokaryotes and is critical for enhanced production of useful metabolites. To increase the glucose uptake rate, we propose a rational strategy for designing the molecular architecture of the Escherichia coli glucose PTS by using a computer‐aided design (CAD) system and verified the simulated results with biological experiments. CAD supports construction of a biochemical map, mathematical modeling, simulation, and system analysis. Assuming that the PTS aims at controlling the glucose uptake rate, the PTS was decomposed into hierarchical modules, functional and flux modules, and the effect of changes in gene expression on the glucose uptake rate was simulated to make a rational strategy of how the gene regulatory network is engineered. Such design and analysis predicted that the mlc knockout mutant with ptsI gene overexpression would greatly increase the specific glucose uptake rate. By using biological experiments, we validated the prediction and the presented strategy, thereby enhancing the specific glucose uptake rate.https://doi.org/10.1038/msb4100201Escherichia coliglucose uptakephosphotransferase systemregulationsystem analysis |
| spellingShingle | Yousuke Nishio Yoshihiro Usuda Kazuhiko Matsui Hiroyuki Kurata Computer‐aided rational design of the phosphotransferase system for enhanced glucose uptake in Escherichia coli Molecular Systems Biology Escherichia coli glucose uptake phosphotransferase system regulation system analysis |
| title | Computer‐aided rational design of the phosphotransferase system for enhanced glucose uptake in Escherichia coli |
| title_full | Computer‐aided rational design of the phosphotransferase system for enhanced glucose uptake in Escherichia coli |
| title_fullStr | Computer‐aided rational design of the phosphotransferase system for enhanced glucose uptake in Escherichia coli |
| title_full_unstemmed | Computer‐aided rational design of the phosphotransferase system for enhanced glucose uptake in Escherichia coli |
| title_short | Computer‐aided rational design of the phosphotransferase system for enhanced glucose uptake in Escherichia coli |
| title_sort | computer aided rational design of the phosphotransferase system for enhanced glucose uptake in escherichia coli |
| topic | Escherichia coli glucose uptake phosphotransferase system regulation system analysis |
| url | https://doi.org/10.1038/msb4100201 |
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