Quantitative analysis of the effect of microtubule-targeting drugs on the microtubule cytoskeleton of breast cancer cells with different invasive properties
The characterization of microtubule-targeting drugs at the cellular level is an essential step in the development of drugs targeting the microtubule network. To that aim, we have previously developed a quantitative cell-based assay easy to perform in microplates that requires only a luminescence rea...
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| Format: | Article |
| Language: | English |
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Académie des sciences
2023-09-01
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| Series: | Comptes Rendus. Chimie |
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| Online Access: | https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.247/ |
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| author | Michallet, Sophie Bosc, Lauriane Lafanechère, Laurence |
| author_facet | Michallet, Sophie Bosc, Lauriane Lafanechère, Laurence |
| author_sort | Michallet, Sophie |
| collection | DOAJ |
| description | The characterization of microtubule-targeting drugs at the cellular level is an essential step in the development of drugs targeting the microtubule network. To that aim, we have previously developed a quantitative cell-based assay easy to perform in microplates that requires only a luminescence reader and no microscopic analysis. Here, we show that this assay can be easily adapted to different breast cancer cell lines. An ideal application of this test could be the comparative analysis of the response of human tumor samples to different microtubule targeting drugs, to optimize therapeutic treatment. |
| format | Article |
| id | doaj-art-2a13ef884d124124b8c8f7924c12ba0e |
| institution | Kabale University |
| issn | 1878-1543 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Académie des sciences |
| record_format | Article |
| series | Comptes Rendus. Chimie |
| spelling | doaj-art-2a13ef884d124124b8c8f7924c12ba0e2025-02-07T13:38:53ZengAcadémie des sciencesComptes Rendus. Chimie1878-15432023-09-0126S391610.5802/crchim.24710.5802/crchim.247Quantitative analysis of the effect of microtubule-targeting drugs on the microtubule cytoskeleton of breast cancer cells with different invasive propertiesMichallet, Sophie0https://orcid.org/0000-0001-9324-7958Bosc, Lauriane1https://orcid.org/0000-0001-7242-9541Lafanechère, Laurence2https://orcid.org/0000-0002-7902-7630Université Grenoble Alpes, INSERM U1209, CNRS UMR5309, Institute for Advanced Biosciences, Team Cytoskeleton Dynamics and Nuclear Functions, 38000 Grenoble, FranceUniversité Grenoble Alpes, INSERM U1209, CNRS UMR5309, Institute for Advanced Biosciences, Team Cytoskeleton Dynamics and Nuclear Functions, 38000 Grenoble, FranceUniversité Grenoble Alpes, INSERM U1209, CNRS UMR5309, Institute for Advanced Biosciences, Team Cytoskeleton Dynamics and Nuclear Functions, 38000 Grenoble, FranceThe characterization of microtubule-targeting drugs at the cellular level is an essential step in the development of drugs targeting the microtubule network. To that aim, we have previously developed a quantitative cell-based assay easy to perform in microplates that requires only a luminescence reader and no microscopic analysis. Here, we show that this assay can be easily adapted to different breast cancer cell lines. An ideal application of this test could be the comparative analysis of the response of human tumor samples to different microtubule targeting drugs, to optimize therapeutic treatment.https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.247/TaxanesVinca-alkaloidsCombretastatin-A4MicrotubulesQuantitative cell-based assayBreast cancer |
| spellingShingle | Michallet, Sophie Bosc, Lauriane Lafanechère, Laurence Quantitative analysis of the effect of microtubule-targeting drugs on the microtubule cytoskeleton of breast cancer cells with different invasive properties Comptes Rendus. Chimie Taxanes Vinca-alkaloids Combretastatin-A4 Microtubules Quantitative cell-based assay Breast cancer |
| title | Quantitative analysis of the effect of microtubule-targeting drugs on the microtubule cytoskeleton of breast cancer cells with different invasive properties |
| title_full | Quantitative analysis of the effect of microtubule-targeting drugs on the microtubule cytoskeleton of breast cancer cells with different invasive properties |
| title_fullStr | Quantitative analysis of the effect of microtubule-targeting drugs on the microtubule cytoskeleton of breast cancer cells with different invasive properties |
| title_full_unstemmed | Quantitative analysis of the effect of microtubule-targeting drugs on the microtubule cytoskeleton of breast cancer cells with different invasive properties |
| title_short | Quantitative analysis of the effect of microtubule-targeting drugs on the microtubule cytoskeleton of breast cancer cells with different invasive properties |
| title_sort | quantitative analysis of the effect of microtubule targeting drugs on the microtubule cytoskeleton of breast cancer cells with different invasive properties |
| topic | Taxanes Vinca-alkaloids Combretastatin-A4 Microtubules Quantitative cell-based assay Breast cancer |
| url | https://comptes-rendus.academie-sciences.fr/chimie/articles/10.5802/crchim.247/ |
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