Polymerase Chain Reaction for the Detection of Histamine-Producing Histophilus somni Isolated from Basra Cattle
This study sought to examine the significance of Histophilus somni as a producer of histamine and a respiratory tract pathogen. Conventional bacteriological and molecular techniques were used to examine 200 nasal swab samples for the presence of Histophilus somni and its ability to produce histamine...
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| Format: | Article |
| Language: | English |
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College of Veterinary Medicine, University of Basrah, Iraq
2024-06-01
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| Series: | Basrah Journal of Veterinary Research |
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| Online Access: | https://bjvr.uobasrah.edu.iq/article_184087_fb651748a2471675fe7ffb239f201132.pdf |
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| _version_ | 1849321477176819712 |
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| author | Raghad Jebur Fawzia Abdullah Jalal Mustafa |
| author_facet | Raghad Jebur Fawzia Abdullah Jalal Mustafa |
| author_sort | Raghad Jebur |
| collection | DOAJ |
| description | This study sought to examine the significance of Histophilus somni as a producer of histamine and a respiratory tract pathogen. Conventional bacteriological and molecular techniques were used to examine 200 nasal swab samples for the presence of Histophilus somni and its ability to produce histamine. Out of 200 cattle nasal swabs tested, 25% (50/200) tested positive for conventional biochemical properties and culture. Histophilus somni was found in 70% (35/50) of the isolates that tested positive for the PCR test after culturing. It was found that 20% (7/35) of the 35 H. somni positive PCR isolates produced positive products when the histidine decarboxylase gene was amplified. Cattle that were infected in the first age group (less than one year) had higher percentages of culture (72%; 36/50) and positive PCR results (80%; 28/35), based on their age. Males demonstrated higher culture (82%;41/50) and PCR (85%; 30/35) positivity percentages regarding the impact of sex. There was a statistically significant difference (P<0.05) between the genders and between the two age groups. |
| format | Article |
| id | doaj-art-2a0ddfa367884e44b977e205b08b0237 |
| institution | Kabale University |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
| record_format | Article |
| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-2a0ddfa367884e44b977e205b08b02372025-08-20T03:49:45ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562024-06-0123214916110.23975/bjvr.2024.149689.1086184087Polymerase Chain Reaction for the Detection of Histamine-Producing Histophilus somni Isolated from Basra CattleRaghad Jebur0Fawzia Abdullah1Jalal Mustafa2College of Veterinary Medicine, University of BasrahCollege of Veterinary Medicine, University of BasrahCollege of Veterinary Medicine, University of BasrahThis study sought to examine the significance of Histophilus somni as a producer of histamine and a respiratory tract pathogen. Conventional bacteriological and molecular techniques were used to examine 200 nasal swab samples for the presence of Histophilus somni and its ability to produce histamine. Out of 200 cattle nasal swabs tested, 25% (50/200) tested positive for conventional biochemical properties and culture. Histophilus somni was found in 70% (35/50) of the isolates that tested positive for the PCR test after culturing. It was found that 20% (7/35) of the 35 H. somni positive PCR isolates produced positive products when the histidine decarboxylase gene was amplified. Cattle that were infected in the first age group (less than one year) had higher percentages of culture (72%; 36/50) and positive PCR results (80%; 28/35), based on their age. Males demonstrated higher culture (82%;41/50) and PCR (85%; 30/35) positivity percentages regarding the impact of sex. There was a statistically significant difference (P<0.05) between the genders and between the two age groups.https://bjvr.uobasrah.edu.iq/article_184087_fb651748a2471675fe7ffb239f201132.pdfpcrhistophilus somnihistamine |
| spellingShingle | Raghad Jebur Fawzia Abdullah Jalal Mustafa Polymerase Chain Reaction for the Detection of Histamine-Producing Histophilus somni Isolated from Basra Cattle Basrah Journal of Veterinary Research pcr histophilus somni histamine |
| title | Polymerase Chain Reaction for the Detection of Histamine-Producing Histophilus somni Isolated from Basra Cattle |
| title_full | Polymerase Chain Reaction for the Detection of Histamine-Producing Histophilus somni Isolated from Basra Cattle |
| title_fullStr | Polymerase Chain Reaction for the Detection of Histamine-Producing Histophilus somni Isolated from Basra Cattle |
| title_full_unstemmed | Polymerase Chain Reaction for the Detection of Histamine-Producing Histophilus somni Isolated from Basra Cattle |
| title_short | Polymerase Chain Reaction for the Detection of Histamine-Producing Histophilus somni Isolated from Basra Cattle |
| title_sort | polymerase chain reaction for the detection of histamine producing histophilus somni isolated from basra cattle |
| topic | pcr histophilus somni histamine |
| url | https://bjvr.uobasrah.edu.iq/article_184087_fb651748a2471675fe7ffb239f201132.pdf |
| work_keys_str_mv | AT raghadjebur polymerasechainreactionforthedetectionofhistamineproducinghistophilussomniisolatedfrombasracattle AT fawziaabdullah polymerasechainreactionforthedetectionofhistamineproducinghistophilussomniisolatedfrombasracattle AT jalalmustafa polymerasechainreactionforthedetectionofhistamineproducinghistophilussomniisolatedfrombasracattle |