Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis.
Candida albicans is a medically important pathogen, and recognition by innate immune cells is critical for its clearance. Although a number of pattern recognition receptors have been shown to be involved in recognition and phagocytosis of this fungus, the relative role of these receptors has not bee...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-11-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1000218&type=printable |
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| author | Sigrid E M Heinsbroek Philip R Taylor Fernando O Martinez Luisa Martinez-Pomares Gordon D Brown Siamon Gordon |
| author_facet | Sigrid E M Heinsbroek Philip R Taylor Fernando O Martinez Luisa Martinez-Pomares Gordon D Brown Siamon Gordon |
| author_sort | Sigrid E M Heinsbroek |
| collection | DOAJ |
| description | Candida albicans is a medically important pathogen, and recognition by innate immune cells is critical for its clearance. Although a number of pattern recognition receptors have been shown to be involved in recognition and phagocytosis of this fungus, the relative role of these receptors has not been formally examined. In this paper, we have investigated the contribution of the mannose receptor, Dectin-1, and complement receptor 3; and we have demonstrated that Dectin-1 is the main non-opsonic receptor involved in fungal uptake. However, both Dectin-1 and complement receptor 3 were found to accumulate at the site of uptake, while mannose receptor accumulated on C. albicans phagosomes at later stages. These results suggest a potential role for MR in phagosome sampling; and, accordingly, MR deficiency led to a reduction in TNF-alpha and MCP-1 production in response to C. albicans uptake. Our data suggest that pattern recognition receptors sample the fungal phagosome in a sequential fashion. |
| format | Article |
| id | doaj-art-2a06872eff3349ebb4b9f32f40b83c84 |
| institution | DOAJ |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2008-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-2a06872eff3349ebb4b9f32f40b83c842025-08-20T03:22:26ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742008-11-01411e100021810.1371/journal.ppat.1000218Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis.Sigrid E M HeinsbroekPhilip R TaylorFernando O MartinezLuisa Martinez-PomaresGordon D BrownSiamon GordonCandida albicans is a medically important pathogen, and recognition by innate immune cells is critical for its clearance. Although a number of pattern recognition receptors have been shown to be involved in recognition and phagocytosis of this fungus, the relative role of these receptors has not been formally examined. In this paper, we have investigated the contribution of the mannose receptor, Dectin-1, and complement receptor 3; and we have demonstrated that Dectin-1 is the main non-opsonic receptor involved in fungal uptake. However, both Dectin-1 and complement receptor 3 were found to accumulate at the site of uptake, while mannose receptor accumulated on C. albicans phagosomes at later stages. These results suggest a potential role for MR in phagosome sampling; and, accordingly, MR deficiency led to a reduction in TNF-alpha and MCP-1 production in response to C. albicans uptake. Our data suggest that pattern recognition receptors sample the fungal phagosome in a sequential fashion.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1000218&type=printable |
| spellingShingle | Sigrid E M Heinsbroek Philip R Taylor Fernando O Martinez Luisa Martinez-Pomares Gordon D Brown Siamon Gordon Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis. PLoS Pathogens |
| title | Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis. |
| title_full | Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis. |
| title_fullStr | Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis. |
| title_full_unstemmed | Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis. |
| title_short | Stage-specific sampling by pattern recognition receptors during Candida albicans phagocytosis. |
| title_sort | stage specific sampling by pattern recognition receptors during candida albicans phagocytosis |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1000218&type=printable |
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