Map-based cloning and characterization reveal that an R2R3 MYB gene confers red glume in wheat
Pigment accumulation is an important trait related to wheat domestication, but there remains a limited understanding of its molecular mechanism. The genetic control of the red glume trait by a dominant allele, Rg-B1, on 1BS was reported in the last century, but the underlying gene and its molecular...
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KeAi Communications Co., Ltd.
2025-06-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514124000564 |
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| author | Weiwei Mao Xiaobo Wang Yongming Chen Yongfa Wang Liang Ma Xiaoming Xie Xiaojia Wu Jin Xu Yuqi Zhang Yue Zhao Xuejun Tian Weilong Guo Zhaorong Hu Mingming Xin Yingyin Yao Zhongfu Ni Qixin Sun Huiru Peng |
| author_facet | Weiwei Mao Xiaobo Wang Yongming Chen Yongfa Wang Liang Ma Xiaoming Xie Xiaojia Wu Jin Xu Yuqi Zhang Yue Zhao Xuejun Tian Weilong Guo Zhaorong Hu Mingming Xin Yingyin Yao Zhongfu Ni Qixin Sun Huiru Peng |
| author_sort | Weiwei Mao |
| collection | DOAJ |
| description | Pigment accumulation is an important trait related to wheat domestication, but there remains a limited understanding of its molecular mechanism. The genetic control of the red glume trait by a dominant allele, Rg-B1, on 1BS was reported in the last century, but the underlying gene and its molecular basis remained elusive. Here, we identified TraesTSP1B01G005700 (G57) encoding an R2R3-MYB transcription factor (TF) as the candidate Rg-B1 gene controlling red glume color by a combination of genome-wide association study (GWAS), bulked segregant RNA-sequencing (BSR-Seq), map-based cloning, and RNA-seq. The Rg-B1 locus had zero to five duplicate copies only one of which had high transcriptional activity. Genetic evidence suggested that promoter sequence variation in G57 in the glume leads to high expression of G57, resulting in the red glume phenotype. G57 could bind to the promoters of anthocyanin synthesis genes TaCHS, TaF3ʹH, and TaUFGT, activating their expression and contributing to anthocyanin accumulation in wheat glume. G57 also played a pivotal role in up-regulating expression of genes TaDREB1C and TaFLO2 associated with increased grain weight, thereby causing increased grain weight. Our research offers a better understanding of the molecular basis of red glume in bread wheat. |
| format | Article |
| id | doaj-art-29e6b2bb5fe04efdae4817346078604d |
| institution | Kabale University |
| issn | 2214-5141 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj-art-29e6b2bb5fe04efdae4817346078604d2025-08-20T03:47:13ZengKeAi Communications Co., Ltd.Crop Journal2214-51412025-06-0113388789910.1016/j.cj.2024.03.002Map-based cloning and characterization reveal that an R2R3 MYB gene confers red glume in wheatWeiwei Mao0Xiaobo Wang1Yongming Chen2Yongfa Wang3Liang Ma4Xiaoming Xie5Xiaojia Wu6Jin Xu7Yuqi Zhang8Yue Zhao9Xuejun Tian10Weilong Guo11Zhaorong Hu12Mingming Xin13Yingyin Yao14Zhongfu Ni15Qixin Sun16Huiru Peng17Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaFrontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaCorresponding author.; Frontiers Science Center for Molecular Design Breeding, Key Laboratory of Crop Heterosis and Utilization, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, ChinaPigment accumulation is an important trait related to wheat domestication, but there remains a limited understanding of its molecular mechanism. The genetic control of the red glume trait by a dominant allele, Rg-B1, on 1BS was reported in the last century, but the underlying gene and its molecular basis remained elusive. Here, we identified TraesTSP1B01G005700 (G57) encoding an R2R3-MYB transcription factor (TF) as the candidate Rg-B1 gene controlling red glume color by a combination of genome-wide association study (GWAS), bulked segregant RNA-sequencing (BSR-Seq), map-based cloning, and RNA-seq. The Rg-B1 locus had zero to five duplicate copies only one of which had high transcriptional activity. Genetic evidence suggested that promoter sequence variation in G57 in the glume leads to high expression of G57, resulting in the red glume phenotype. G57 could bind to the promoters of anthocyanin synthesis genes TaCHS, TaF3ʹH, and TaUFGT, activating their expression and contributing to anthocyanin accumulation in wheat glume. G57 also played a pivotal role in up-regulating expression of genes TaDREB1C and TaFLO2 associated with increased grain weight, thereby causing increased grain weight. Our research offers a better understanding of the molecular basis of red glume in bread wheat.http://www.sciencedirect.com/science/article/pii/S2214514124000564AnthocyaninRed glume colorGene duplicationTriticum aestivum |
| spellingShingle | Weiwei Mao Xiaobo Wang Yongming Chen Yongfa Wang Liang Ma Xiaoming Xie Xiaojia Wu Jin Xu Yuqi Zhang Yue Zhao Xuejun Tian Weilong Guo Zhaorong Hu Mingming Xin Yingyin Yao Zhongfu Ni Qixin Sun Huiru Peng Map-based cloning and characterization reveal that an R2R3 MYB gene confers red glume in wheat Crop Journal Anthocyanin Red glume color Gene duplication Triticum aestivum |
| title | Map-based cloning and characterization reveal that an R2R3 MYB gene confers red glume in wheat |
| title_full | Map-based cloning and characterization reveal that an R2R3 MYB gene confers red glume in wheat |
| title_fullStr | Map-based cloning and characterization reveal that an R2R3 MYB gene confers red glume in wheat |
| title_full_unstemmed | Map-based cloning and characterization reveal that an R2R3 MYB gene confers red glume in wheat |
| title_short | Map-based cloning and characterization reveal that an R2R3 MYB gene confers red glume in wheat |
| title_sort | map based cloning and characterization reveal that an r2r3 myb gene confers red glume in wheat |
| topic | Anthocyanin Red glume color Gene duplication Triticum aestivum |
| url | http://www.sciencedirect.com/science/article/pii/S2214514124000564 |
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