Analysis of gliadin patterns and diversity in Triticum polonicum L. accessions from Ethiopia

Gliadins from 25 accessions represented by 350 individual seed samples were analysed by acid-polyacrylamide gel electrophoresis (A-PAGE) with the objective of identifying gliadin band patterns and examine the extent of diversity in Triticum polonicum L. collections from Ethiopia. Seventy polymorphic...

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Main Author: Eleni SHIFERAW
Format: Article
Language:English
Published: University of Ljubljana Press (Založba Univerze v Ljubljani) 2021-03-01
Series:Acta Agriculturae Slovenica
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Online Access:https://journals.uni-lj.si/aas/article/view/12987
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author Eleni SHIFERAW
author_facet Eleni SHIFERAW
author_sort Eleni SHIFERAW
collection DOAJ
description Gliadins from 25 accessions represented by 350 individual seed samples were analysed by acid-polyacrylamide gel electrophoresis (A-PAGE) with the objective of identifying gliadin band patterns and examine the extent of diversity in Triticum polonicum L. collections from Ethiopia. Seventy polymorphic bands and 68 different patterns were identified. Eighteen different mobility bands and 16 patterns were identified in ω-gliadin region, 22 bands and 20 patterns in γ-gliadin region, 12 bands and 22 patterns in β-gliadin region and 18 bands and 10 patterns in α-gliadin region. The average genetic diversity calculated from the data of the four gliadin zones of the analysed samples was 0.15. The γ region have the highest diversity (H = 0.193), followed by ω regions (H = 0.177) and β region (H = 0.168) and the lowest diversity was observed in α region (H = 0.127). Cluster analysis based on genetic distances resulted in grouping of the analysed accessions in to seven main groups. Though the level of diversity was relatively lower than other tetraploid wheat species from Ethiopia, the findings are indicative of the existence of variation in the collections which can be exploited for wheat improvement.
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spelling doaj-art-29d6b4b62f6f4bfaaa1cac08e5e1a2312025-08-20T02:05:13ZengUniversity of Ljubljana Press (Založba Univerze v Ljubljani)Acta Agriculturae Slovenica1854-19412021-03-0111711610.14720/aas.2021.117.1.193519379Analysis of gliadin patterns and diversity in Triticum polonicum L. accessions from EthiopiaEleni SHIFERAW0Ethiopian Biodiversity Institute, Addis Ababa, EthiopiaGliadins from 25 accessions represented by 350 individual seed samples were analysed by acid-polyacrylamide gel electrophoresis (A-PAGE) with the objective of identifying gliadin band patterns and examine the extent of diversity in Triticum polonicum L. collections from Ethiopia. Seventy polymorphic bands and 68 different patterns were identified. Eighteen different mobility bands and 16 patterns were identified in ω-gliadin region, 22 bands and 20 patterns in γ-gliadin region, 12 bands and 22 patterns in β-gliadin region and 18 bands and 10 patterns in α-gliadin region. The average genetic diversity calculated from the data of the four gliadin zones of the analysed samples was 0.15. The γ region have the highest diversity (H = 0.193), followed by ω regions (H = 0.177) and β region (H = 0.168) and the lowest diversity was observed in α region (H = 0.127). Cluster analysis based on genetic distances resulted in grouping of the analysed accessions in to seven main groups. Though the level of diversity was relatively lower than other tetraploid wheat species from Ethiopia, the findings are indicative of the existence of variation in the collections which can be exploited for wheat improvement.https://journals.uni-lj.si/aas/article/view/12987a-pagegenetic diversitygliadintriticum polonicum
spellingShingle Eleni SHIFERAW
Analysis of gliadin patterns and diversity in Triticum polonicum L. accessions from Ethiopia
Acta Agriculturae Slovenica
a-page
genetic diversity
gliadin
triticum polonicum
title Analysis of gliadin patterns and diversity in Triticum polonicum L. accessions from Ethiopia
title_full Analysis of gliadin patterns and diversity in Triticum polonicum L. accessions from Ethiopia
title_fullStr Analysis of gliadin patterns and diversity in Triticum polonicum L. accessions from Ethiopia
title_full_unstemmed Analysis of gliadin patterns and diversity in Triticum polonicum L. accessions from Ethiopia
title_short Analysis of gliadin patterns and diversity in Triticum polonicum L. accessions from Ethiopia
title_sort analysis of gliadin patterns and diversity in triticum polonicum l accessions from ethiopia
topic a-page
genetic diversity
gliadin
triticum polonicum
url https://journals.uni-lj.si/aas/article/view/12987
work_keys_str_mv AT elenishiferaw analysisofgliadinpatternsanddiversityintriticumpolonicumlaccessionsfromethiopia