Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro
Cessation of blood supply due to myocardial infarction (MI) leads to complicated pathological alteration in the affected regions. Cardiac stem cells (CSCs) migration plays a major role in promoting recovery of cardiac function and protecting cardiomyocytes in post-MI remodeling. Despite being the mo...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2015/836390 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849307818838982656 |
|---|---|
| author | Qing Gao Maojuan Guo Wenyun Zeng Yijing Wang Lin Yang Xiaoli Pang Huhu Li Yanrong Suo Xijuan Jiang Chunquan Yu |
| author_facet | Qing Gao Maojuan Guo Wenyun Zeng Yijing Wang Lin Yang Xiaoli Pang Huhu Li Yanrong Suo Xijuan Jiang Chunquan Yu |
| author_sort | Qing Gao |
| collection | DOAJ |
| description | Cessation of blood supply due to myocardial infarction (MI) leads to complicated pathological alteration in the affected regions. Cardiac stem cells (CSCs) migration plays a major role in promoting recovery of cardiac function and protecting cardiomyocytes in post-MI remodeling. Despite being the most abundant cell type in the mammalian heart, cardiac fibroblasts (CFs) were underestimated in the mechanism of CSCs migration. Our objective in this study is therefore to investigate the migration related factors secreted by hypoxia CFs in vitro and the degree that they contribute to CSCs migration. We found that supernatant from hypoxia induced CFs could accelerate CSCs migration. Four migration-related cytokines were reported upregulated both in mRNA and protein levels. Upon adding antagonists of these cytokines, the number of migration cells significantly declined. When the cocktail antagonists of all above four cytokines were added, the migration cells number reduced to the minimum level. Besides, MMP-9 had an important effect on triggering CSCs migration. As shown in our results, MMP-9 induced CSCs migration and the underlying mechanism might involve TNF-α signaling which induced VEGF and MMP-9 expression. |
| format | Article |
| id | doaj-art-29d43cf5cc3f4e739e640eb9e8464c28 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-29d43cf5cc3f4e739e640eb9e8464c282025-08-20T03:54:37ZengWileyStem Cells International1687-966X1687-96782015-01-01201510.1155/2015/836390836390Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In VitroQing Gao0Maojuan Guo1Wenyun Zeng2Yijing Wang3Lin Yang4Xiaoli Pang5Huhu Li6Yanrong Suo7Xijuan Jiang8Chunquan Yu9School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaSchool of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaSchool of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaSchool of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaSchool of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaSchool of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaSchool of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaSchool of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaSchool of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaPeriodical Newsroom, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaCessation of blood supply due to myocardial infarction (MI) leads to complicated pathological alteration in the affected regions. Cardiac stem cells (CSCs) migration plays a major role in promoting recovery of cardiac function and protecting cardiomyocytes in post-MI remodeling. Despite being the most abundant cell type in the mammalian heart, cardiac fibroblasts (CFs) were underestimated in the mechanism of CSCs migration. Our objective in this study is therefore to investigate the migration related factors secreted by hypoxia CFs in vitro and the degree that they contribute to CSCs migration. We found that supernatant from hypoxia induced CFs could accelerate CSCs migration. Four migration-related cytokines were reported upregulated both in mRNA and protein levels. Upon adding antagonists of these cytokines, the number of migration cells significantly declined. When the cocktail antagonists of all above four cytokines were added, the migration cells number reduced to the minimum level. Besides, MMP-9 had an important effect on triggering CSCs migration. As shown in our results, MMP-9 induced CSCs migration and the underlying mechanism might involve TNF-α signaling which induced VEGF and MMP-9 expression.http://dx.doi.org/10.1155/2015/836390 |
| spellingShingle | Qing Gao Maojuan Guo Wenyun Zeng Yijing Wang Lin Yang Xiaoli Pang Huhu Li Yanrong Suo Xijuan Jiang Chunquan Yu Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro Stem Cells International |
| title | Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro |
| title_full | Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro |
| title_fullStr | Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro |
| title_full_unstemmed | Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro |
| title_short | Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro |
| title_sort | matrix metalloproteinase 9 secreted by hypoxia cardiac fibroblasts triggers cardiac stem cell migration in vitro |
| url | http://dx.doi.org/10.1155/2015/836390 |
| work_keys_str_mv | AT qinggao matrixmetalloproteinase9secretedbyhypoxiacardiacfibroblaststriggerscardiacstemcellmigrationinvitro AT maojuanguo matrixmetalloproteinase9secretedbyhypoxiacardiacfibroblaststriggerscardiacstemcellmigrationinvitro AT wenyunzeng matrixmetalloproteinase9secretedbyhypoxiacardiacfibroblaststriggerscardiacstemcellmigrationinvitro AT yijingwang matrixmetalloproteinase9secretedbyhypoxiacardiacfibroblaststriggerscardiacstemcellmigrationinvitro AT linyang matrixmetalloproteinase9secretedbyhypoxiacardiacfibroblaststriggerscardiacstemcellmigrationinvitro AT xiaolipang matrixmetalloproteinase9secretedbyhypoxiacardiacfibroblaststriggerscardiacstemcellmigrationinvitro AT huhuli matrixmetalloproteinase9secretedbyhypoxiacardiacfibroblaststriggerscardiacstemcellmigrationinvitro AT yanrongsuo matrixmetalloproteinase9secretedbyhypoxiacardiacfibroblaststriggerscardiacstemcellmigrationinvitro AT xijuanjiang matrixmetalloproteinase9secretedbyhypoxiacardiacfibroblaststriggerscardiacstemcellmigrationinvitro AT chunquanyu matrixmetalloproteinase9secretedbyhypoxiacardiacfibroblaststriggerscardiacstemcellmigrationinvitro |