Clinical validation of serum immunosignatures in early diagnosis of Crohn's disease
Abstract Background The search for biomarkers suitable for early diagnosis of Crohn's disease (CD) is challenging. This study investigated the efficacy of serological markers for the early diagnosis of CD. Methods This was a retrospective nested cohort study. Indirect immunofluorescence and enz...
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Wiley
2023-12-01
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| Series: | iLabmed |
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| Online Access: | https://doi.org/10.1002/ila2.28 |
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| author | Xianzong Ma Wenyu Zhang Xin Wang Lang Yang Juan Jiao Yan Jia Dezhi Wang Junfeng Xu Peng Jin Mingjie Zhang Shirong Li Yuanming Pan Jianqiu Sheng |
| author_facet | Xianzong Ma Wenyu Zhang Xin Wang Lang Yang Juan Jiao Yan Jia Dezhi Wang Junfeng Xu Peng Jin Mingjie Zhang Shirong Li Yuanming Pan Jianqiu Sheng |
| author_sort | Xianzong Ma |
| collection | DOAJ |
| description | Abstract Background The search for biomarkers suitable for early diagnosis of Crohn's disease (CD) is challenging. This study investigated the efficacy of serological markers for the early diagnosis of CD. Methods This was a retrospective nested cohort study. Indirect immunofluorescence and enzyme‐linked immunosorbent assay were used to detect ASCA IgG, ASCA IgA, AYMA IgG, AYCA IgG, FI2Y IgG, p‐ANCA IgG, GAB IgG and PAB IgG in patient serum samples. Results The positive rates of ASCA IgG, ASCA IgA, AYMA IgG, AYCA IgG, FI2Y IgG, p‐ANCA IgG, GAB IgG and PAB IgG in patients with early CD, advanced CD and other intestinal diseases were 37.0% versus 56.8% versus 27.8%; 3.7% versus 20.5% versus 19.4%; 14.8% versus 2.3% versus 2.8%; 25.9% versus 9.1% versus 8.3%; 18.5% versus 15.9% versus 8.3%; 0.0% versus 2.8%, 18.5%; 13.6% versus 18.2% versus 16.7%; and 7.4% versus 20.5% versus 0.0%, respectively. The positive rates of ASCA IgG, AYCA IgG and PAB IgG were significantly different among the three groups (p < 0.05). In 85.2% of early CD patients, at least one antibody was detected 1 year before diagnosis. The sensitivity of the ASCA/AYMA/AYCA/FI2Y/GAB combination for early diagnosis was 85.2%. The sensitivity of the ASCA/AYMA/AYCA/FI2Y/GAB/PAB/PANCA combination for differentiating CD from other diseases was 87.3%. Conclusions ASCA IgG and AYCA IgG have potential value in identifying the course of CD. AYCA IgG may be a potential marker for the early diagnosis of CD, and ASCA IgG indicates an advanced stage. The combination of ASCA, AYMA, AYCA, FI2Y, and GAB improves early diagnostic accuracy of CD. |
| format | Article |
| id | doaj-art-29cea375af05499d8f9e617f29db0fc0 |
| institution | Kabale University |
| issn | 2834-443X 2834-4448 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | iLabmed |
| spelling | doaj-art-29cea375af05499d8f9e617f29db0fc02025-08-20T03:40:13ZengWileyiLabmed2834-443X2834-44482023-12-011318118910.1002/ila2.28Clinical validation of serum immunosignatures in early diagnosis of Crohn's diseaseXianzong Ma0Wenyu Zhang1Xin Wang2Lang Yang3Juan Jiao4Yan Jia5Dezhi Wang6Junfeng Xu7Peng Jin8Mingjie Zhang9Shirong Li10Yuanming Pan11Jianqiu Sheng12Medical School of Chinese PLA Chinese PLA General Hospital Beijing ChinaCapital Medical University Beijing ChinaDepartment of Gastroenterology The Seventh Medical Center of Chinese PLA General Hospital Beijing ChinaDepartment of Gastroenterology The Seventh Medical Center of Chinese PLA General Hospital Beijing ChinaLaboratory Medicine Center The Seventh Medical Center of Chinese PLA General Hospital Beijing ChinaDepartment of Gastroenterology The Seventh Medical Center of Chinese PLA General Hospital Beijing ChinaMedical School of Chinese PLA Chinese PLA General Hospital Beijing ChinaSenior Department of Gastroenterology The First Medical Center of Chinese PLA General Hospital Beijing ChinaDepartment of Gastroenterology The Seventh Medical Center of Chinese PLA General Hospital Beijing ChinaDepartment of Gastroenterology The Seventh Medical Center of Chinese PLA General Hospital Beijing ChinaDepartment of Gastroenterology The Seventh Medical Center of Chinese PLA General Hospital Beijing ChinaCancer Research Center Beijing Chest Hospital Capital Medical University Beijing ChinaMedical School of Chinese PLA Chinese PLA General Hospital Beijing ChinaAbstract Background The search for biomarkers suitable for early diagnosis of Crohn's disease (CD) is challenging. This study investigated the efficacy of serological markers for the early diagnosis of CD. Methods This was a retrospective nested cohort study. Indirect immunofluorescence and enzyme‐linked immunosorbent assay were used to detect ASCA IgG, ASCA IgA, AYMA IgG, AYCA IgG, FI2Y IgG, p‐ANCA IgG, GAB IgG and PAB IgG in patient serum samples. Results The positive rates of ASCA IgG, ASCA IgA, AYMA IgG, AYCA IgG, FI2Y IgG, p‐ANCA IgG, GAB IgG and PAB IgG in patients with early CD, advanced CD and other intestinal diseases were 37.0% versus 56.8% versus 27.8%; 3.7% versus 20.5% versus 19.4%; 14.8% versus 2.3% versus 2.8%; 25.9% versus 9.1% versus 8.3%; 18.5% versus 15.9% versus 8.3%; 0.0% versus 2.8%, 18.5%; 13.6% versus 18.2% versus 16.7%; and 7.4% versus 20.5% versus 0.0%, respectively. The positive rates of ASCA IgG, AYCA IgG and PAB IgG were significantly different among the three groups (p < 0.05). In 85.2% of early CD patients, at least one antibody was detected 1 year before diagnosis. The sensitivity of the ASCA/AYMA/AYCA/FI2Y/GAB combination for early diagnosis was 85.2%. The sensitivity of the ASCA/AYMA/AYCA/FI2Y/GAB/PAB/PANCA combination for differentiating CD from other diseases was 87.3%. Conclusions ASCA IgG and AYCA IgG have potential value in identifying the course of CD. AYCA IgG may be a potential marker for the early diagnosis of CD, and ASCA IgG indicates an advanced stage. The combination of ASCA, AYMA, AYCA, FI2Y, and GAB improves early diagnostic accuracy of CD.https://doi.org/10.1002/ila2.28Crohn's diseaseearly diagnosisserological markersAYCAASCA |
| spellingShingle | Xianzong Ma Wenyu Zhang Xin Wang Lang Yang Juan Jiao Yan Jia Dezhi Wang Junfeng Xu Peng Jin Mingjie Zhang Shirong Li Yuanming Pan Jianqiu Sheng Clinical validation of serum immunosignatures in early diagnosis of Crohn's disease iLabmed Crohn's disease early diagnosis serological markers AYCA ASCA |
| title | Clinical validation of serum immunosignatures in early diagnosis of Crohn's disease |
| title_full | Clinical validation of serum immunosignatures in early diagnosis of Crohn's disease |
| title_fullStr | Clinical validation of serum immunosignatures in early diagnosis of Crohn's disease |
| title_full_unstemmed | Clinical validation of serum immunosignatures in early diagnosis of Crohn's disease |
| title_short | Clinical validation of serum immunosignatures in early diagnosis of Crohn's disease |
| title_sort | clinical validation of serum immunosignatures in early diagnosis of crohn s disease |
| topic | Crohn's disease early diagnosis serological markers AYCA ASCA |
| url | https://doi.org/10.1002/ila2.28 |
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