Detection of canine parvovirus type 2c (CPV-2c) in Palestine
Introduction: The objective of the present study was to report, for the first time, the presence of canine parvovirus type 2c (CPV-2c) in domesticated dogs with acute gastroenteritis and to characterize the antigenic variants circulating in Palestine. Methodology: A veterinary clinical-based epid...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2024-05-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/18835 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849324115114065920 |
|---|---|
| author | Ibrahim M Alzuheir Adnan F Fayyad Belal Y Abu Helal Hatem A Atalla Nasr H Jalboush |
| author_facet | Ibrahim M Alzuheir Adnan F Fayyad Belal Y Abu Helal Hatem A Atalla Nasr H Jalboush |
| author_sort | Ibrahim M Alzuheir |
| collection | DOAJ |
| description |
Introduction: The objective of the present study was to report, for the first time, the presence of canine parvovirus type 2c (CPV-2c) in domesticated dogs with acute gastroenteritis and to characterize the antigenic variants circulating in Palestine.
Methodology: A veterinary clinical-based epidemiological study was carried out between December 2022 and April 2023. Fifty fecal samples were collected from dogs with gastroenteritis and screened for CPV-2 infection by polymerase chain reaction. The distribution of positive cases according to various epidemiological factors was studied. Partial sequencing of the viral protein 2 (VP2) gene was performed for the analysis of CPV-2 variants.
Results: Most of the investigated samples (60%; n = 50) during the study period were found positive for CPV-2 infection. There was no difference in the distribution of positive cases of CPV-2 infection based on age group, gender, location, and vaccination status. The analysis of nucleotide and amino acid sequences from amplified products, as well as phylogenetic analysis, revealed the presence of CPV-2c clustered with Asian CPV-2c variants.
Conclusions: In summary, this study represents the initial genetic analysis of CPV-2 present in Palestinian dogs with gastroenteritis and provides evidence that confirms the existence of the CPV-2c variants. To determine the prevailing CPV-2 variant associated with the infection, it is crucial to conduct further sequence analysis using large populations of both domestic and wild canines.
|
| format | Article |
| id | doaj-art-29cbc8a200f64df39bdf38b02daf8035 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-29cbc8a200f64df39bdf38b02daf80352025-08-20T03:48:47ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802024-05-01180510.3855/jidc.18835Detection of canine parvovirus type 2c (CPV-2c) in PalestineIbrahim M Alzuheir0Adnan F Fayyad1Belal Y Abu Helal2Hatem A Atalla3Nasr H Jalboush4Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, An-Najah National University, P.O. Box 7, Nablus, PalestineDepartment of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, An-Najah National University, P.O. Box 7, Nablus, PalestineDr. Belal Abu Helal Veterinary Clinic, Tulkarm, P304, PalestineDepartment of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, An-Najah National University, P.O. Box 7, Nablus, PalestineDepartment of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, An-Najah National University, P.O. Box 7, Nablus, Palestine Introduction: The objective of the present study was to report, for the first time, the presence of canine parvovirus type 2c (CPV-2c) in domesticated dogs with acute gastroenteritis and to characterize the antigenic variants circulating in Palestine. Methodology: A veterinary clinical-based epidemiological study was carried out between December 2022 and April 2023. Fifty fecal samples were collected from dogs with gastroenteritis and screened for CPV-2 infection by polymerase chain reaction. The distribution of positive cases according to various epidemiological factors was studied. Partial sequencing of the viral protein 2 (VP2) gene was performed for the analysis of CPV-2 variants. Results: Most of the investigated samples (60%; n = 50) during the study period were found positive for CPV-2 infection. There was no difference in the distribution of positive cases of CPV-2 infection based on age group, gender, location, and vaccination status. The analysis of nucleotide and amino acid sequences from amplified products, as well as phylogenetic analysis, revealed the presence of CPV-2c clustered with Asian CPV-2c variants. Conclusions: In summary, this study represents the initial genetic analysis of CPV-2 present in Palestinian dogs with gastroenteritis and provides evidence that confirms the existence of the CPV-2c variants. To determine the prevailing CPV-2 variant associated with the infection, it is crucial to conduct further sequence analysis using large populations of both domestic and wild canines. https://jidc.org/index.php/journal/article/view/18835CPV-2cdogsPalestinephylogenetic analysisVP2 genecanine parvovirus |
| spellingShingle | Ibrahim M Alzuheir Adnan F Fayyad Belal Y Abu Helal Hatem A Atalla Nasr H Jalboush Detection of canine parvovirus type 2c (CPV-2c) in Palestine Journal of Infection in Developing Countries CPV-2c dogs Palestine phylogenetic analysis VP2 gene canine parvovirus |
| title | Detection of canine parvovirus type 2c (CPV-2c) in Palestine |
| title_full | Detection of canine parvovirus type 2c (CPV-2c) in Palestine |
| title_fullStr | Detection of canine parvovirus type 2c (CPV-2c) in Palestine |
| title_full_unstemmed | Detection of canine parvovirus type 2c (CPV-2c) in Palestine |
| title_short | Detection of canine parvovirus type 2c (CPV-2c) in Palestine |
| title_sort | detection of canine parvovirus type 2c cpv 2c in palestine |
| topic | CPV-2c dogs Palestine phylogenetic analysis VP2 gene canine parvovirus |
| url | https://jidc.org/index.php/journal/article/view/18835 |
| work_keys_str_mv | AT ibrahimmalzuheir detectionofcanineparvovirustype2ccpv2cinpalestine AT adnanffayyad detectionofcanineparvovirustype2ccpv2cinpalestine AT belalyabuhelal detectionofcanineparvovirustype2ccpv2cinpalestine AT hatemaatalla detectionofcanineparvovirustype2ccpv2cinpalestine AT nasrhjalboush detectionofcanineparvovirustype2ccpv2cinpalestine |