Survival of probiotic microorganisms in the gastrointestinal tract of experimental animals
Development of methodology for the identification of certified probiotic microorganisms in the intestinal contents of white mice and uinea pigs and study of their survival in the gastrointestinal tract of experimental animals. Rifampicin-resistant bifidobacteria and lactobacilli were used in the exp...
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| Format: | Article |
| Language: | Russian |
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Journal Infectology
2014-09-01
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| Series: | Журнал инфектологии |
| Subjects: | |
| Online Access: | https://journal.niidi.ru/jofin/article/view/88 |
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| author | I. V. Darmov I. Yu. Chicherin I. P. Pogorelsky I. A. Lundovskikh E. A. Durnev |
| author_facet | I. V. Darmov I. Yu. Chicherin I. P. Pogorelsky I. A. Lundovskikh E. A. Durnev |
| author_sort | I. V. Darmov |
| collection | DOAJ |
| description | Development of methodology for the identification of certified probiotic microorganisms in the intestinal contents of white mice and uinea pigs and study of their survival in the gastrointestinal tract of experimental animals. Rifampicin-resistant bifidobacteria and lactobacilli were used in the experiments. Cultures of microorganisms that have retained the species features were administered orally for 14 days and the number of viable bifidobacteria and lactobacilli were determined by sowing of feces in a dense nutrient medium with rifampicin. Probiotic microorganisms administered orally to experimental animals for 14 days are detected in the feces on the second day of the experiment. Live probiotic bacteria ceases completely to be detected in the feces of animals 3 days after the termination of their oral administration. Using the developed universal method of differentiation of probiotic microorganisms entering the living organisms and strains of their own intestinal microflora a significant decrease (4–7 orders of magnitude) in survival of bifidobacteria and lactobacilli in the organisms of experimental animals was shown, followed by a lack of probiotic effect. |
| format | Article |
| id | doaj-art-29c7203ebbff4d72befdaf05bea31e05 |
| institution | Kabale University |
| issn | 2072-6732 |
| language | Russian |
| publishDate | 2014-09-01 |
| publisher | Journal Infectology |
| record_format | Article |
| series | Журнал инфектологии |
| spelling | doaj-art-29c7203ebbff4d72befdaf05bea31e052025-08-20T04:00:07ZrusJournal InfectologyЖурнал инфектологии2072-67322014-09-0141687410.22625/2072-6732-2012-4-1-68-74102Survival of probiotic microorganisms in the gastrointestinal tract of experimental animalsI. V. Darmov0I. Yu. Chicherin1I. P. Pogorelsky2I. A. Lundovskikh3E. A. Durnev4Vyatka State University, KirovLLC «MedStar»Vyatka State University, KirovVyatka State University, KirovVyatka State University, KirovDevelopment of methodology for the identification of certified probiotic microorganisms in the intestinal contents of white mice and uinea pigs and study of their survival in the gastrointestinal tract of experimental animals. Rifampicin-resistant bifidobacteria and lactobacilli were used in the experiments. Cultures of microorganisms that have retained the species features were administered orally for 14 days and the number of viable bifidobacteria and lactobacilli were determined by sowing of feces in a dense nutrient medium with rifampicin. Probiotic microorganisms administered orally to experimental animals for 14 days are detected in the feces on the second day of the experiment. Live probiotic bacteria ceases completely to be detected in the feces of animals 3 days after the termination of their oral administration. Using the developed universal method of differentiation of probiotic microorganisms entering the living organisms and strains of their own intestinal microflora a significant decrease (4–7 orders of magnitude) in survival of bifidobacteria and lactobacilli in the organisms of experimental animals was shown, followed by a lack of probiotic effect.https://journal.niidi.ru/jofin/article/view/88probiotic microorganismsintestinal microflorasurvival in the gastrointestinal tractresistance of bacteria to rifampicinwhite miceguinea pigs |
| spellingShingle | I. V. Darmov I. Yu. Chicherin I. P. Pogorelsky I. A. Lundovskikh E. A. Durnev Survival of probiotic microorganisms in the gastrointestinal tract of experimental animals Журнал инфектологии probiotic microorganisms intestinal microflora survival in the gastrointestinal tract resistance of bacteria to rifampicin white mice guinea pigs |
| title | Survival of probiotic microorganisms in the gastrointestinal tract of experimental animals |
| title_full | Survival of probiotic microorganisms in the gastrointestinal tract of experimental animals |
| title_fullStr | Survival of probiotic microorganisms in the gastrointestinal tract of experimental animals |
| title_full_unstemmed | Survival of probiotic microorganisms in the gastrointestinal tract of experimental animals |
| title_short | Survival of probiotic microorganisms in the gastrointestinal tract of experimental animals |
| title_sort | survival of probiotic microorganisms in the gastrointestinal tract of experimental animals |
| topic | probiotic microorganisms intestinal microflora survival in the gastrointestinal tract resistance of bacteria to rifampicin white mice guinea pigs |
| url | https://journal.niidi.ru/jofin/article/view/88 |
| work_keys_str_mv | AT ivdarmov survivalofprobioticmicroorganismsinthegastrointestinaltractofexperimentalanimals AT iyuchicherin survivalofprobioticmicroorganismsinthegastrointestinaltractofexperimentalanimals AT ippogorelsky survivalofprobioticmicroorganismsinthegastrointestinaltractofexperimentalanimals AT ialundovskikh survivalofprobioticmicroorganismsinthegastrointestinaltractofexperimentalanimals AT eadurnev survivalofprobioticmicroorganismsinthegastrointestinaltractofexperimentalanimals |