Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues.
Leptospirosis is an extremely widespread zoonotic infection with outcomes ranging from subclinical infection to fatal Weil's syndrome. Despite the global impact of the disease, key aspects of its pathogenesis remain unclear. To examine in detail the earliest steps in the host response to leptos...
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| Language: | English |
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Public Library of Science (PLoS)
2009-06-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0000463&type=printable |
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| author | J Muse Davis David A Haake Lalita Ramakrishnan |
| author_facet | J Muse Davis David A Haake Lalita Ramakrishnan |
| author_sort | J Muse Davis |
| collection | DOAJ |
| description | Leptospirosis is an extremely widespread zoonotic infection with outcomes ranging from subclinical infection to fatal Weil's syndrome. Despite the global impact of the disease, key aspects of its pathogenesis remain unclear. To examine in detail the earliest steps in the host response to leptospires, we used fluorescently labelled Leptospira interrogans serovar Copenhageni to infect 30 hour post fertilization zebrafish embryos by either the caudal vein or hindbrain ventricle. These embryos have functional innate immunity but have not yet developed an adaptive immune system. Furthermore, they are optically transparent, allowing direct visualization of host-pathogen interactions from the moment of infection. We observed rapid uptake of leptospires by phagocytes, followed by persistent, intracellular infection over the first 48 hours. Phagocytosis of leptospires occasionally resulted in formation of large cellular vesicles consistent with apoptotic bodies. By 24 hours, clusters of infected phagocytes were accumulating lateral to the dorsal artery, presumably in early hematopoietic tissue. Our observations suggest that phagocytosis may be a key defense mechanism in the early stages of leptospirosis, and that phagocytic cells play roles in immunopathogenesis and likely in the dissemination of leptospires to specific target tissues. |
| format | Article |
| id | doaj-art-29c3d263b13b42c7ad12c02b8b184708 |
| institution | OA Journals |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2009-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-29c3d263b13b42c7ad12c02b8b1847082025-08-20T02:00:13ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352009-06-0136e46310.1371/journal.pntd.0000463Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues.J Muse DavisDavid A HaakeLalita RamakrishnanLeptospirosis is an extremely widespread zoonotic infection with outcomes ranging from subclinical infection to fatal Weil's syndrome. Despite the global impact of the disease, key aspects of its pathogenesis remain unclear. To examine in detail the earliest steps in the host response to leptospires, we used fluorescently labelled Leptospira interrogans serovar Copenhageni to infect 30 hour post fertilization zebrafish embryos by either the caudal vein or hindbrain ventricle. These embryos have functional innate immunity but have not yet developed an adaptive immune system. Furthermore, they are optically transparent, allowing direct visualization of host-pathogen interactions from the moment of infection. We observed rapid uptake of leptospires by phagocytes, followed by persistent, intracellular infection over the first 48 hours. Phagocytosis of leptospires occasionally resulted in formation of large cellular vesicles consistent with apoptotic bodies. By 24 hours, clusters of infected phagocytes were accumulating lateral to the dorsal artery, presumably in early hematopoietic tissue. Our observations suggest that phagocytosis may be a key defense mechanism in the early stages of leptospirosis, and that phagocytic cells play roles in immunopathogenesis and likely in the dissemination of leptospires to specific target tissues.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0000463&type=printable |
| spellingShingle | J Muse Davis David A Haake Lalita Ramakrishnan Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. PLoS Neglected Tropical Diseases |
| title | Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. |
| title_full | Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. |
| title_fullStr | Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. |
| title_full_unstemmed | Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. |
| title_short | Leptospira interrogans stably infects zebrafish embryos, altering phagocyte behavior and homing to specific tissues. |
| title_sort | leptospira interrogans stably infects zebrafish embryos altering phagocyte behavior and homing to specific tissues |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0000463&type=printable |
| work_keys_str_mv | AT jmusedavis leptospirainterrogansstablyinfectszebrafishembryosalteringphagocytebehaviorandhomingtospecifictissues AT davidahaake leptospirainterrogansstablyinfectszebrafishembryosalteringphagocytebehaviorandhomingtospecifictissues AT lalitaramakrishnan leptospirainterrogansstablyinfectszebrafishembryosalteringphagocytebehaviorandhomingtospecifictissues |