Association between magnesium depletion score and Klotho levels among U.S. adults: Findings from NHANES 2007–2016

Background: Magnesium is a vital mineral that plays a crucial role in various physiological processes, and its imbalance has been associated with many chronic aging-related diseases. Traditional assessment methods are insufficient, and the Magnesium Depletion Score (MDS) has been proposed as a signi...

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Main Authors: Xiaoyi Ma, Yuxin Yang, Kemin Yan, Lei Su, Jin Li, Yingying Gong, Wen He
Format: Article
Language:English
Published: Elsevier 2025-02-01
Series:Heliyon
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405844025011909
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author Xiaoyi Ma
Yuxin Yang
Kemin Yan
Lei Su
Jin Li
Yingying Gong
Wen He
author_facet Xiaoyi Ma
Yuxin Yang
Kemin Yan
Lei Su
Jin Li
Yingying Gong
Wen He
author_sort Xiaoyi Ma
collection DOAJ
description Background: Magnesium is a vital mineral that plays a crucial role in various physiological processes, and its imbalance has been associated with many chronic aging-related diseases. Traditional assessment methods are insufficient, and the Magnesium Depletion Score (MDS) has been proposed as a significant indicator. Klotho, a transmembrane protein, is involved in aging regulation and oxidative stress modulation. Despite the shared relevance of MDS and Klotho to aging, their relationship remains unclear. Aims: This study aims to investigate the relationship between MDS and Klotho levels in U.S. adults. Methods: We performed a cross-sectional analysis utilizing information from the NHANES database (2007–2016), including 8285 participants. MDS was computed according to specific criteria, encompassing alcohol intake, proton pump inhibitor utilization, diuretic utilization, and renal function. Participants were categorized into groups according to MDS score: none to low (score = 0 or 1), medium (score = 2), and high (score >2). The association between MDS and Klotho levels was assessed using weighted multivariable linear regression models and subgroup analysis. Results: The analysis revealed a negative association between MDS and serum Klotho levels after comprehensive adjustment. Participants with the highest MDS showed decreased Klotho concentrations compared to those with the lowest level. This association remained robust even after excluding outliers. Subgroup analysis indicated a stronger correlation between MDS and serum Klotho concentration in individuals aged 40–60 years, women, and those with normal weight. Conclusion: The findings suggest a negative association between MDS and Klotho levels, indicating that magnesium metabolism may influence Klotho expression. These results underscore the potential significance of magnesium in aging processes and age-related diseases, highlighting the potential benefit of increasing serum Klotho concentration through magnesium supplementation.
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spelling doaj-art-29c339b7fd694089aa2848b67879c2fb2025-08-20T03:13:18ZengElsevierHeliyon2405-84402025-02-01114e4280910.1016/j.heliyon.2025.e42809Association between magnesium depletion score and Klotho levels among U.S. adults: Findings from NHANES 2007–2016Xiaoyi Ma0Yuxin Yang1Kemin Yan2Lei Su3Jin Li4Yingying Gong5Wen He6Department of Geriatrics, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, PR China; Corresponding author. Department of Geriatrics, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, PR China.Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, PR ChinaDepartment of Geriatrics, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, PR ChinaDepartment of Geriatrics, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, PR ChinaDepartment of Geriatrics, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, PR ChinaDepartment of Geriatrics, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, PR ChinaDepartment of Geriatrics, The First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510080, PR China; Corresponding author. Department of Geriatrics, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, PR China.Background: Magnesium is a vital mineral that plays a crucial role in various physiological processes, and its imbalance has been associated with many chronic aging-related diseases. Traditional assessment methods are insufficient, and the Magnesium Depletion Score (MDS) has been proposed as a significant indicator. Klotho, a transmembrane protein, is involved in aging regulation and oxidative stress modulation. Despite the shared relevance of MDS and Klotho to aging, their relationship remains unclear. Aims: This study aims to investigate the relationship between MDS and Klotho levels in U.S. adults. Methods: We performed a cross-sectional analysis utilizing information from the NHANES database (2007–2016), including 8285 participants. MDS was computed according to specific criteria, encompassing alcohol intake, proton pump inhibitor utilization, diuretic utilization, and renal function. Participants were categorized into groups according to MDS score: none to low (score = 0 or 1), medium (score = 2), and high (score >2). The association between MDS and Klotho levels was assessed using weighted multivariable linear regression models and subgroup analysis. Results: The analysis revealed a negative association between MDS and serum Klotho levels after comprehensive adjustment. Participants with the highest MDS showed decreased Klotho concentrations compared to those with the lowest level. This association remained robust even after excluding outliers. Subgroup analysis indicated a stronger correlation between MDS and serum Klotho concentration in individuals aged 40–60 years, women, and those with normal weight. Conclusion: The findings suggest a negative association between MDS and Klotho levels, indicating that magnesium metabolism may influence Klotho expression. These results underscore the potential significance of magnesium in aging processes and age-related diseases, highlighting the potential benefit of increasing serum Klotho concentration through magnesium supplementation.http://www.sciencedirect.com/science/article/pii/S2405844025011909Magnesium depletion scoreKlothoAgingMagnesium metabolismNHANES
spellingShingle Xiaoyi Ma
Yuxin Yang
Kemin Yan
Lei Su
Jin Li
Yingying Gong
Wen He
Association between magnesium depletion score and Klotho levels among U.S. adults: Findings from NHANES 2007–2016
Heliyon
Magnesium depletion score
Klotho
Aging
Magnesium metabolism
NHANES
title Association between magnesium depletion score and Klotho levels among U.S. adults: Findings from NHANES 2007–2016
title_full Association between magnesium depletion score and Klotho levels among U.S. adults: Findings from NHANES 2007–2016
title_fullStr Association between magnesium depletion score and Klotho levels among U.S. adults: Findings from NHANES 2007–2016
title_full_unstemmed Association between magnesium depletion score and Klotho levels among U.S. adults: Findings from NHANES 2007–2016
title_short Association between magnesium depletion score and Klotho levels among U.S. adults: Findings from NHANES 2007–2016
title_sort association between magnesium depletion score and klotho levels among u s adults findings from nhanes 2007 2016
topic Magnesium depletion score
Klotho
Aging
Magnesium metabolism
NHANES
url http://www.sciencedirect.com/science/article/pii/S2405844025011909
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