Apoptotic cell death, detected ex vivo in peripheral blood lymphocytes of HIV-1 infected persons
In HIV-1 infection the ongoing depletion of CD4+ T-lymphocytes is believed, to a large extent, to be due to apoptosis. Until now quantitative information about in vivo apoptosis of lymphocytes in HIV-patients is scarce because of the very nature of the apoptotic process. Successful detection of apop...
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| Format: | Article |
| Language: | English |
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Wiley
1996-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935196000543 |
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| author | L. F. te Velde I. Vermes C. Haanen C. P. M. Reutelingsperger C. H. H. ten Napel |
| author_facet | L. F. te Velde I. Vermes C. Haanen C. P. M. Reutelingsperger C. H. H. ten Napel |
| author_sort | L. F. te Velde |
| collection | DOAJ |
| description | In HIV-1 infection the ongoing depletion of CD4+ T-lymphocytes is believed, to a large extent, to be due to apoptosis. Until now quantitative information about in vivo apoptosis of lymphocytes in HIV-patients is scarce because of the very nature of the apoptotic process. Successful detection of apoptosis ex vivo requires the recognition of the initial phase of this process, because at a later stage the cells may not remain any longer in the circulation. We measured quantitatively the amount of early apoptotic peripheral blood lymphocytes directly ex vivo in HIV-1 infected patients using a recently described flow cytometric assay. With this method we observed in an unselected heterogenous group of twelve HIV-infected individuals a median percentage of apoptotic lymphocytes to be significantly higher than in ten healthy controls. To the best of our knowledge this is the first report of ex vivo observed increased apoptosis of peripheral blood lymphocytes in HIV-infected persons. |
| format | Article |
| id | doaj-art-29c26bcefc774a04b274cedcc62bf5c1 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1996-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-29c26bcefc774a04b274cedcc62bf5c12025-02-03T05:57:09ZengWileyMediators of Inflammation0962-93511466-18611996-01-015537938110.1155/S0962935196000543Apoptotic cell death, detected ex vivo in peripheral blood lymphocytes of HIV-1 infected personsL. F. te Velde0I. Vermes1C. Haanen2C. P. M. Reutelingsperger3C. H. H. ten Napel4Department of Internal Medicine, Oncology and AIDS section, Medical Spectrum Twente, Hospital Group, Enschede, The NetherlandsDepartment of Clinical Chemistry, Medical Spectrum Twente, Hospital Group, Enschede, The NetherlandsDepartment of Clinical Chemistry, Medical Spectrum Twente, Hospital Group, Enschede, The NetherlandsDepartment of Biochemistry, University of Limburg, Maastricht, The NetherlandsDepartment of Internal Medicine, Oncology and AIDS section, Medical Spectrum Twente, Hospital Group, Enschede, The NetherlandsIn HIV-1 infection the ongoing depletion of CD4+ T-lymphocytes is believed, to a large extent, to be due to apoptosis. Until now quantitative information about in vivo apoptosis of lymphocytes in HIV-patients is scarce because of the very nature of the apoptotic process. Successful detection of apoptosis ex vivo requires the recognition of the initial phase of this process, because at a later stage the cells may not remain any longer in the circulation. We measured quantitatively the amount of early apoptotic peripheral blood lymphocytes directly ex vivo in HIV-1 infected patients using a recently described flow cytometric assay. With this method we observed in an unselected heterogenous group of twelve HIV-infected individuals a median percentage of apoptotic lymphocytes to be significantly higher than in ten healthy controls. To the best of our knowledge this is the first report of ex vivo observed increased apoptosis of peripheral blood lymphocytes in HIV-infected persons.http://dx.doi.org/10.1155/S0962935196000543 |
| spellingShingle | L. F. te Velde I. Vermes C. Haanen C. P. M. Reutelingsperger C. H. H. ten Napel Apoptotic cell death, detected ex vivo in peripheral blood lymphocytes of HIV-1 infected persons Mediators of Inflammation |
| title | Apoptotic cell death, detected ex vivo in peripheral blood lymphocytes of HIV-1 infected persons |
| title_full | Apoptotic cell death, detected ex vivo in peripheral blood lymphocytes of HIV-1 infected persons |
| title_fullStr | Apoptotic cell death, detected ex vivo in peripheral blood lymphocytes of HIV-1 infected persons |
| title_full_unstemmed | Apoptotic cell death, detected ex vivo in peripheral blood lymphocytes of HIV-1 infected persons |
| title_short | Apoptotic cell death, detected ex vivo in peripheral blood lymphocytes of HIV-1 infected persons |
| title_sort | apoptotic cell death detected ex vivo in peripheral blood lymphocytes of hiv 1 infected persons |
| url | http://dx.doi.org/10.1155/S0962935196000543 |
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