The Detection of Lymph Node Metastasis Metastases Using a Near-Infrared Fluorescent Probe Based on Tumor-Targeted Monoclonal Antibody Antibodies Specific: A Prospective Study in a Nude Mouse Model
Background: To identify the metastatic lymph nodes and remove them accurately, the fluorescent surgical navigation ability of the ovarian cancer-specific fluorescent probe COC183B2-800 was assessed to verify the metastatic lymph nodes in the nude mouse model....
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| Format: | Article |
| Language: | English |
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IMR Press
2024-09-01
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| Series: | Clinical and Experimental Obstetrics & Gynecology |
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| Online Access: | https://www.imrpress.com/journal/CEOG/51/9/10.31083/j.ceog5109211 |
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| author | Huarui Yang Yijun Zhang Chongyuan Zhu Yi Zhu Xuelian Song Xiaohong Chang Yi Li |
| author_facet | Huarui Yang Yijun Zhang Chongyuan Zhu Yi Zhu Xuelian Song Xiaohong Chang Yi Li |
| author_sort | Huarui Yang |
| collection | DOAJ |
| description | Background: To identify the metastatic lymph nodes and remove them accurately, the fluorescent surgical navigation ability of the ovarian cancer-specific fluorescent probe COC183B2-800 was assessed to verify the metastatic lymph nodes in the nude mouse model. Methods: The nude mouse model related to lymph node metastases in human ovarian cancer was established using the SKOV3-ip1 cell line. Besides, the COC183B2-800 probe (IRDye800CW Ester conjugated COC183B2 antibody) was fabricated. Moreover, in vivo fluorescence imaging was performed to determine the ability of the COC183B2-800 fluorescent probe to identify metastatic lymph nodes in the nude mouse model. Results: The nude mouse model related to lymph node metastases in human ovarian cancer was successfully established. In vivo fluorescence imaging was performed 30 hours after the injection of the COC183B2-800 fluorescent probe (25 μg) into the animal model, which can achieve specific imaging of metastatic lymph nodes. All metastatic lymph nodes were detected in vivo and in vitro (8/8), and only 1 negative lymph node with reactive enlargement showed a false positive fluorescent signal. Conclusions: The targeted fluorescent probe COC183B2-800 can be employed to identify metastatic lymph nodes in the nude mouse model related to lymph node metastases in human ovarian cancer with high specificity and sensitivity. Targeted fluorescence imaging using COC183B2-800 is expected to become a method to achieve precise lymphadenectomy. |
| format | Article |
| id | doaj-art-29bfc2a9a9b34c55a951b8f4f2f83610 |
| institution | Kabale University |
| issn | 0390-6663 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Clinical and Experimental Obstetrics & Gynecology |
| spelling | doaj-art-29bfc2a9a9b34c55a951b8f4f2f836102025-08-20T03:54:37ZengIMR PressClinical and Experimental Obstetrics & Gynecology0390-66632024-09-0151921110.31083/j.ceog5109211S0390-6663(24)02430-8The Detection of Lymph Node Metastasis Metastases Using a Near-Infrared Fluorescent Probe Based on Tumor-Targeted Monoclonal Antibody Antibodies Specific: A Prospective Study in a Nude Mouse ModelHuarui Yang0Yijun Zhang1Chongyuan Zhu2Yi Zhu3Xuelian Song4Xiaohong Chang5Yi Li6Department of Obstetrics and Gynecology, Peking University People’s Hospital, 100044 Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University People’s Hospital, 100044 Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University People’s Hospital, 100044 Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University People’s Hospital, 100044 Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University People’s Hospital, 100044 Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University People’s Hospital, 100044 Beijing, ChinaDepartment of Obstetrics and Gynecology, Peking University People’s Hospital, 100044 Beijing, ChinaBackground: To identify the metastatic lymph nodes and remove them accurately, the fluorescent surgical navigation ability of the ovarian cancer-specific fluorescent probe COC183B2-800 was assessed to verify the metastatic lymph nodes in the nude mouse model. Methods: The nude mouse model related to lymph node metastases in human ovarian cancer was established using the SKOV3-ip1 cell line. Besides, the COC183B2-800 probe (IRDye800CW Ester conjugated COC183B2 antibody) was fabricated. Moreover, in vivo fluorescence imaging was performed to determine the ability of the COC183B2-800 fluorescent probe to identify metastatic lymph nodes in the nude mouse model. Results: The nude mouse model related to lymph node metastases in human ovarian cancer was successfully established. In vivo fluorescence imaging was performed 30 hours after the injection of the COC183B2-800 fluorescent probe (25 μg) into the animal model, which can achieve specific imaging of metastatic lymph nodes. All metastatic lymph nodes were detected in vivo and in vitro (8/8), and only 1 negative lymph node with reactive enlargement showed a false positive fluorescent signal. Conclusions: The targeted fluorescent probe COC183B2-800 can be employed to identify metastatic lymph nodes in the nude mouse model related to lymph node metastases in human ovarian cancer with high specificity and sensitivity. Targeted fluorescence imaging using COC183B2-800 is expected to become a method to achieve precise lymphadenectomy.https://www.imrpress.com/journal/CEOG/51/9/10.31083/j.ceog5109211ovarian cancerlymph node metastasisanimal modelfluorescence-guided surgerycoc183b2 |
| spellingShingle | Huarui Yang Yijun Zhang Chongyuan Zhu Yi Zhu Xuelian Song Xiaohong Chang Yi Li The Detection of Lymph Node Metastasis Metastases Using a Near-Infrared Fluorescent Probe Based on Tumor-Targeted Monoclonal Antibody Antibodies Specific: A Prospective Study in a Nude Mouse Model Clinical and Experimental Obstetrics & Gynecology ovarian cancer lymph node metastasis animal model fluorescence-guided surgery coc183b2 |
| title | The Detection of Lymph Node Metastasis Metastases Using a Near-Infrared Fluorescent Probe Based on Tumor-Targeted Monoclonal Antibody Antibodies Specific: A Prospective Study in a Nude Mouse Model |
| title_full | The Detection of Lymph Node Metastasis Metastases Using a Near-Infrared Fluorescent Probe Based on Tumor-Targeted Monoclonal Antibody Antibodies Specific: A Prospective Study in a Nude Mouse Model |
| title_fullStr | The Detection of Lymph Node Metastasis Metastases Using a Near-Infrared Fluorescent Probe Based on Tumor-Targeted Monoclonal Antibody Antibodies Specific: A Prospective Study in a Nude Mouse Model |
| title_full_unstemmed | The Detection of Lymph Node Metastasis Metastases Using a Near-Infrared Fluorescent Probe Based on Tumor-Targeted Monoclonal Antibody Antibodies Specific: A Prospective Study in a Nude Mouse Model |
| title_short | The Detection of Lymph Node Metastasis Metastases Using a Near-Infrared Fluorescent Probe Based on Tumor-Targeted Monoclonal Antibody Antibodies Specific: A Prospective Study in a Nude Mouse Model |
| title_sort | detection of lymph node metastasis metastases using a near infrared fluorescent probe based on tumor targeted monoclonal antibody antibodies specific a prospective study in a nude mouse model |
| topic | ovarian cancer lymph node metastasis animal model fluorescence-guided surgery coc183b2 |
| url | https://www.imrpress.com/journal/CEOG/51/9/10.31083/j.ceog5109211 |
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