Detection of morphine and data processing using surface plasmon resonance imaging sensor
Based on the surface plasmon resonance imaging (SPRi) instrument, we established a new method of analyzing morphine in urine by processing a calibration curve. According to an inhibition immunoassay, gradient concentration of morphine and morphine-BSA fixed on the chip competitively combine with mor...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2024-10-01
|
| Series: | Green Processing and Synthesis |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/gps-2024-0067 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850268392429191168 |
|---|---|
| author | Sun Jianuo Ke Haokun Wang Jinghan Du Xianchao Hao Hongxia Zhou Hong |
| author_facet | Sun Jianuo Ke Haokun Wang Jinghan Du Xianchao Hao Hongxia Zhou Hong |
| author_sort | Sun Jianuo |
| collection | DOAJ |
| description | Based on the surface plasmon resonance imaging (SPRi) instrument, we established a new method of analyzing morphine in urine by processing a calibration curve. According to an inhibition immunoassay, gradient concentration of morphine and morphine-BSA fixed on the chip competitively combine with morphine antibody on the chip. Given the three mathematical models, the data of SPRi signals generated from SPRi with morphine was processed to obtain the calibration curve. Ultrafiltration was used to pretreat blank urine samples with adding morphine, and then investigated the advantages and disadvantages of each model. With a limit detection of 6.57 ng·mL−1, the method and mathematical models can provide robust support for SPRi sensors used in further environmental detection, such as the epidemiological study of sewage. |
| format | Article |
| id | doaj-art-29b7d700666d4ac2a60ab2c610c64be9 |
| institution | OA Journals |
| issn | 2191-9550 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Green Processing and Synthesis |
| spelling | doaj-art-29b7d700666d4ac2a60ab2c610c64be92025-08-20T01:53:30ZengDe GruyterGreen Processing and Synthesis2191-95502024-10-0113172810.1515/gps-2024-0067Detection of morphine and data processing using surface plasmon resonance imaging sensorSun Jianuo0Ke Haokun1Wang Jinghan2Du Xianchao3Hao Hongxia4Zhou Hong5Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing100088, ChinaKey Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing100088, ChinaKey Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing100088, ChinaCollege of Chemistry and Pharmaceutical Engineering, Nanyang Normal University, Nanyang, Henan, 473061, ChinaKey Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing100088, ChinaInstitute of Forensic Science, Ministry of Public Security, Beijing, 100038, ChinaBased on the surface plasmon resonance imaging (SPRi) instrument, we established a new method of analyzing morphine in urine by processing a calibration curve. According to an inhibition immunoassay, gradient concentration of morphine and morphine-BSA fixed on the chip competitively combine with morphine antibody on the chip. Given the three mathematical models, the data of SPRi signals generated from SPRi with morphine was processed to obtain the calibration curve. Ultrafiltration was used to pretreat blank urine samples with adding morphine, and then investigated the advantages and disadvantages of each model. With a limit detection of 6.57 ng·mL−1, the method and mathematical models can provide robust support for SPRi sensors used in further environmental detection, such as the epidemiological study of sewage.https://doi.org/10.1515/gps-2024-0067spriinhibition immunoassaymorphinecalibration curve |
| spellingShingle | Sun Jianuo Ke Haokun Wang Jinghan Du Xianchao Hao Hongxia Zhou Hong Detection of morphine and data processing using surface plasmon resonance imaging sensor Green Processing and Synthesis spri inhibition immunoassay morphine calibration curve |
| title | Detection of morphine and data processing using surface plasmon resonance imaging sensor |
| title_full | Detection of morphine and data processing using surface plasmon resonance imaging sensor |
| title_fullStr | Detection of morphine and data processing using surface plasmon resonance imaging sensor |
| title_full_unstemmed | Detection of morphine and data processing using surface plasmon resonance imaging sensor |
| title_short | Detection of morphine and data processing using surface plasmon resonance imaging sensor |
| title_sort | detection of morphine and data processing using surface plasmon resonance imaging sensor |
| topic | spri inhibition immunoassay morphine calibration curve |
| url | https://doi.org/10.1515/gps-2024-0067 |
| work_keys_str_mv | AT sunjianuo detectionofmorphineanddataprocessingusingsurfaceplasmonresonanceimagingsensor AT kehaokun detectionofmorphineanddataprocessingusingsurfaceplasmonresonanceimagingsensor AT wangjinghan detectionofmorphineanddataprocessingusingsurfaceplasmonresonanceimagingsensor AT duxianchao detectionofmorphineanddataprocessingusingsurfaceplasmonresonanceimagingsensor AT haohongxia detectionofmorphineanddataprocessingusingsurfaceplasmonresonanceimagingsensor AT zhouhong detectionofmorphineanddataprocessingusingsurfaceplasmonresonanceimagingsensor |