Metabolomics combined with physiology and transcriptomics reveal the regulation of key nitrogen metabolic pathways in alfalfa by foliar spraying with nano-selenium
Abstract Selenium promotes plant growth and improves nutritional quality, and the role of nano-selenium in alfalfa in regulating nutritional quality is unknown. In this study, using the 15N labeling method, it was found that nano-selenium could promote plant nitrogen metabolism and photosynthesis by...
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2025-01-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-024-03073-4 |
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| author | Pengbo Sun Gentu Ge Lin Sun Jian Bao Muqier Zhao Junfeng Hao Yuhan Zhang Guoshun Liu Zhijun Wang Yushan Jia |
| author_facet | Pengbo Sun Gentu Ge Lin Sun Jian Bao Muqier Zhao Junfeng Hao Yuhan Zhang Guoshun Liu Zhijun Wang Yushan Jia |
| author_sort | Pengbo Sun |
| collection | DOAJ |
| description | Abstract Selenium promotes plant growth and improves nutritional quality, and the role of nano-selenium in alfalfa in regulating nutritional quality is unknown. In this study, using the 15N labeling method, it was found that nano-selenium could promote plant nitrogen metabolism and photosynthesis by increasing the light energy capture capacity and the activities of key enzymes of the nitrogen metabolism process, leading to an increase in alfalfa nitrogen accumulation and dry matter content. The transcriptome and metabolome revealed that nano-selenium mainly affected the pathways of ‘biosynthesis of amino acids’, ‘starch and sucrose metabolism’, ‘pentose and glucuronate interconversions’, ‘pentose phosphate pathway’, and ‘flavonoid biosynthesis’. At the early stage of nano-selenium treatment, the nitrogen metabolism, sugar metabolism, and flavonoid metabolism pathways were regulated by modulating the expression of genes such as NR, Nir, GS, GOGAT, E3.1.1.11, adh, CHS, FLS, etc., which increased the amount of L-glutamic, L-histidine, glycerone-P, coniferin, naringenin chalcone, and other beneficial substances, thus promoting the acceleration of nitrogen accumulation by plants. In summary, this study provides a better understanding of the mechanisms by which nano-selenium regulates key nitrogen metabolic pathways in alfalfa. Graphical Abstract |
| format | Article |
| id | doaj-art-299db4ff78994efbb6ddce7ecb06c06e |
| institution | OA Journals |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| spelling | doaj-art-299db4ff78994efbb6ddce7ecb06c06e2025-08-20T01:47:59ZengBMCJournal of Nanobiotechnology1477-31552025-01-0123112110.1186/s12951-024-03073-4Metabolomics combined with physiology and transcriptomics reveal the regulation of key nitrogen metabolic pathways in alfalfa by foliar spraying with nano-seleniumPengbo Sun0Gentu Ge1Lin Sun2Jian Bao3Muqier Zhao4Junfeng Hao5Yuhan Zhang6Guoshun Liu7Zhijun Wang8Yushan Jia9Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization, Ministry of Agriculture, People’s Republic of China, Key Laboratory of Grassland Resources, Ministry of Education, People’s Republic of China, College of Grassland, Resources and Environment, Inner Mongolia Agricultural UniversityKey Laboratory of Forage Cultivation, Processing and High Efficient Utilization, Ministry of Agriculture, People’s Republic of China, Key Laboratory of Grassland Resources, Ministry of Education, People’s Republic of China, College of Grassland, Resources and Environment, Inner Mongolia Agricultural UniversityInner Mongolia Academy of Agricultural and Animal Husbandry SciencesInner Mongolia Academy of Agricultural and Animal Husbandry SciencesKey Laboratory of Forage Cultivation, Processing and High Efficient Utilization, Ministry of Agriculture, People’s Republic of China, Key Laboratory of Grassland Resources, Ministry of Education, People’s Republic of China, College of Grassland, Resources and Environment, Inner Mongolia Agricultural UniversityKey Laboratory of Forage Cultivation, Processing and High Efficient Utilization, Ministry of Agriculture, People’s Republic of China, Key Laboratory of Grassland Resources, Ministry of Education, People’s Republic of China, College of Grassland, Resources and Environment, Inner Mongolia Agricultural UniversityForestry and Grassland Work Station of Inner MongoliaForestry Station of XiningKey Laboratory of Forage Cultivation, Processing and High Efficient Utilization, Ministry of Agriculture, People’s Republic of China, Key Laboratory of Grassland Resources, Ministry of Education, People’s Republic of China, College of Grassland, Resources and Environment, Inner Mongolia Agricultural UniversityKey Laboratory of Forage Cultivation, Processing and High Efficient Utilization, Ministry of Agriculture, People’s Republic of China, Key Laboratory of Grassland Resources, Ministry of Education, People’s Republic of China, College of Grassland, Resources and Environment, Inner Mongolia Agricultural UniversityAbstract Selenium promotes plant growth and improves nutritional quality, and the role of nano-selenium in alfalfa in regulating nutritional quality is unknown. In this study, using the 15N labeling method, it was found that nano-selenium could promote plant nitrogen metabolism and photosynthesis by increasing the light energy capture capacity and the activities of key enzymes of the nitrogen metabolism process, leading to an increase in alfalfa nitrogen accumulation and dry matter content. The transcriptome and metabolome revealed that nano-selenium mainly affected the pathways of ‘biosynthesis of amino acids’, ‘starch and sucrose metabolism’, ‘pentose and glucuronate interconversions’, ‘pentose phosphate pathway’, and ‘flavonoid biosynthesis’. At the early stage of nano-selenium treatment, the nitrogen metabolism, sugar metabolism, and flavonoid metabolism pathways were regulated by modulating the expression of genes such as NR, Nir, GS, GOGAT, E3.1.1.11, adh, CHS, FLS, etc., which increased the amount of L-glutamic, L-histidine, glycerone-P, coniferin, naringenin chalcone, and other beneficial substances, thus promoting the acceleration of nitrogen accumulation by plants. In summary, this study provides a better understanding of the mechanisms by which nano-selenium regulates key nitrogen metabolic pathways in alfalfa. Graphical Abstracthttps://doi.org/10.1186/s12951-024-03073-4Nano-seleniumAlfalfaNitrogen metabolismTranscriptomeMetabolome |
| spellingShingle | Pengbo Sun Gentu Ge Lin Sun Jian Bao Muqier Zhao Junfeng Hao Yuhan Zhang Guoshun Liu Zhijun Wang Yushan Jia Metabolomics combined with physiology and transcriptomics reveal the regulation of key nitrogen metabolic pathways in alfalfa by foliar spraying with nano-selenium Journal of Nanobiotechnology Nano-selenium Alfalfa Nitrogen metabolism Transcriptome Metabolome |
| title | Metabolomics combined with physiology and transcriptomics reveal the regulation of key nitrogen metabolic pathways in alfalfa by foliar spraying with nano-selenium |
| title_full | Metabolomics combined with physiology and transcriptomics reveal the regulation of key nitrogen metabolic pathways in alfalfa by foliar spraying with nano-selenium |
| title_fullStr | Metabolomics combined with physiology and transcriptomics reveal the regulation of key nitrogen metabolic pathways in alfalfa by foliar spraying with nano-selenium |
| title_full_unstemmed | Metabolomics combined with physiology and transcriptomics reveal the regulation of key nitrogen metabolic pathways in alfalfa by foliar spraying with nano-selenium |
| title_short | Metabolomics combined with physiology and transcriptomics reveal the regulation of key nitrogen metabolic pathways in alfalfa by foliar spraying with nano-selenium |
| title_sort | metabolomics combined with physiology and transcriptomics reveal the regulation of key nitrogen metabolic pathways in alfalfa by foliar spraying with nano selenium |
| topic | Nano-selenium Alfalfa Nitrogen metabolism Transcriptome Metabolome |
| url | https://doi.org/10.1186/s12951-024-03073-4 |
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