Chronic Restraint Stress Induces an Isoform-Specific Regulation on the Neural Cell Adhesion Molecule in the Hippocampus
Existing evidence indicates that 21-days exposure of rats to restraint stress induces dendritic atrophy in pyramidal cells of the hippocampus. This phenomenon has been related to altered performance in hippocampal-dependent learning tasks. Prior studies have shown that hippocampal expression of cell...
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| Format: | Article |
| Language: | English |
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Wiley
2002-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/NP.2002.147 |
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| author | K. Touyarot C. Sandi |
| author_facet | K. Touyarot C. Sandi |
| author_sort | K. Touyarot |
| collection | DOAJ |
| description | Existing evidence indicates that 21-days
exposure of rats to restraint stress induces
dendritic atrophy in pyramidal cells of the
hippocampus. This phenomenon has been
related to altered performance in hippocampal-dependent
learning tasks. Prior studies have
shown that hippocampal expression of cell
adhesion molecules is modified by such stress
treatment, with the neural cell adhesion molecule
(NCAM) decreasing and L1 increasing, their
expression, at both the mRNA and protein
levels. Given that NCAM comprises several
isoforms, we investigated here whether chronic
stress might differentially affect the expression
of the three major isoforms (NCAM-120,
NCAM-140, NCAM-180) in the hippocampus.
In addition, as glucocorticoids have been
implicated in the deleterious effects induced by
chronic stress, we also evaluated plasma
corticosterone levels and the hippocampal
expression of the corticosteroid mineralocorticoid
receptor (MR) and glucocorticoid
receptor (GR). The results showed that the
protein concentration of the NCAM-140
isoform decreased in the hippoampus of
stressed rats. This effect was isoform-specific,
because NCAM-120 and NCAM-180 levels were
not significantly modified. In addition, whereas
basal levels of plasma corticosterone tended to
be increased, MR and GR concentrations were
not significantly altered. Although possible
changes in NCAM-120, NCAM-180 and
corticosteroid receptors at earlier time points of
the stress period cannot be ignored; this study
suggests that a down-regulation of NCAM-140
might be implicated in the structural
alterations consistently shown to be induced in
the hippocampus by chronic stress exposure. As
NCAM-140 is involved in cell-cell adhesion and
neurite outgrowth, these findings suggest that
this molecule might be one of the molecular
mechanisms involved in the complex interactions
among neurodegeneration-related events. |
| format | Article |
| id | doaj-art-2997610fb7a54815b906f9133a3c33ac |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2002-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-2997610fb7a54815b906f9133a3c33ac2025-02-03T06:12:56ZengWileyNeural Plasticity2090-59041687-54432002-01-019314715910.1155/NP.2002.147Chronic Restraint Stress Induces an Isoform-Specific Regulation on the Neural Cell Adhesion Molecule in the HippocampusK. Touyarot0C. Sandi1Dept of Psychobiology, Universidad Nacional de Educacion a Distancia, Ciudad Universitaria s/n, Madrid 28040, SpainDept of Psychobiology, Universidad Nacional de Educacion a Distancia, Ciudad Universitaria s/n, Madrid 28040, SpainExisting evidence indicates that 21-days exposure of rats to restraint stress induces dendritic atrophy in pyramidal cells of the hippocampus. This phenomenon has been related to altered performance in hippocampal-dependent learning tasks. Prior studies have shown that hippocampal expression of cell adhesion molecules is modified by such stress treatment, with the neural cell adhesion molecule (NCAM) decreasing and L1 increasing, their expression, at both the mRNA and protein levels. Given that NCAM comprises several isoforms, we investigated here whether chronic stress might differentially affect the expression of the three major isoforms (NCAM-120, NCAM-140, NCAM-180) in the hippocampus. In addition, as glucocorticoids have been implicated in the deleterious effects induced by chronic stress, we also evaluated plasma corticosterone levels and the hippocampal expression of the corticosteroid mineralocorticoid receptor (MR) and glucocorticoid receptor (GR). The results showed that the protein concentration of the NCAM-140 isoform decreased in the hippoampus of stressed rats. This effect was isoform-specific, because NCAM-120 and NCAM-180 levels were not significantly modified. In addition, whereas basal levels of plasma corticosterone tended to be increased, MR and GR concentrations were not significantly altered. Although possible changes in NCAM-120, NCAM-180 and corticosteroid receptors at earlier time points of the stress period cannot be ignored; this study suggests that a down-regulation of NCAM-140 might be implicated in the structural alterations consistently shown to be induced in the hippocampus by chronic stress exposure. As NCAM-140 is involved in cell-cell adhesion and neurite outgrowth, these findings suggest that this molecule might be one of the molecular mechanisms involved in the complex interactions among neurodegeneration-related events.http://dx.doi.org/10.1155/NP.2002.147 |
| spellingShingle | K. Touyarot C. Sandi Chronic Restraint Stress Induces an Isoform-Specific Regulation on the Neural Cell Adhesion Molecule in the Hippocampus Neural Plasticity |
| title | Chronic Restraint Stress Induces an Isoform-Specific Regulation
on the Neural Cell Adhesion Molecule in the Hippocampus |
| title_full | Chronic Restraint Stress Induces an Isoform-Specific Regulation
on the Neural Cell Adhesion Molecule in the Hippocampus |
| title_fullStr | Chronic Restraint Stress Induces an Isoform-Specific Regulation
on the Neural Cell Adhesion Molecule in the Hippocampus |
| title_full_unstemmed | Chronic Restraint Stress Induces an Isoform-Specific Regulation
on the Neural Cell Adhesion Molecule in the Hippocampus |
| title_short | Chronic Restraint Stress Induces an Isoform-Specific Regulation
on the Neural Cell Adhesion Molecule in the Hippocampus |
| title_sort | chronic restraint stress induces an isoform specific regulation on the neural cell adhesion molecule in the hippocampus |
| url | http://dx.doi.org/10.1155/NP.2002.147 |
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