Expression of AMPK and PLIN2 in the regulation of lipid metabolism and oxidative stress in bitches with open cervix pyometra
Abstract The pathogenesis of canine pyometra is multifactorial, involving hormonal imbalances, aberrant immune responses, and metabolic dysregulation includes lipid metabolism and oxidative stress. This study focuses on lipid metabolism and oxidative stress, revealing the key regulatory role of AMPK...
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BMC
2025-03-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-025-04622-1 |
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| author | Xin Deng Hui Liu Wei Zhao Rui Wu Kuo Chen Qing Li Murat Onur Yazlık Hüseyin Özkan Jingyuan Ren Jiacheng Zhang Shiyi Liu Ling Mei Shangfeng Li Jiasui Zhan Binhong Hu |
| author_facet | Xin Deng Hui Liu Wei Zhao Rui Wu Kuo Chen Qing Li Murat Onur Yazlık Hüseyin Özkan Jingyuan Ren Jiacheng Zhang Shiyi Liu Ling Mei Shangfeng Li Jiasui Zhan Binhong Hu |
| author_sort | Xin Deng |
| collection | DOAJ |
| description | Abstract The pathogenesis of canine pyometra is multifactorial, involving hormonal imbalances, aberrant immune responses, and metabolic dysregulation includes lipid metabolism and oxidative stress. This study focuses on lipid metabolism and oxidative stress, revealing the key regulatory role of AMPK and PLIN2 in canine pyometra. Bitches with open cervix pyometra (n:8) and healthy bitches undergoing elective ovariohysterectomy (n:4) were enrolled to the study. In experiment one, the serum and tissue levels of Malondialdehyde (MDA) and Superoxide Dismutase (SOD) activity were assessed. Additionally, uterine histopathological analysis, AMPK and PLIN2 expressions were determined through immunohistochemistry. Furthermore, inflammation, oxidative stress, and lipid metabolism-related factors were evaluated using Western blot analysis. In experiment two, primary cell cultures were prepared from healthy uterine endometrial cells of the dogs in control group. Cultured canine endometrial epithelial cells were treated with lipopolysaccharide (LPS) along with oleic acid (OA) to induce an inflammatory response. Tissue and serum MDA and SOD levels were greater in dogs with pyometra. Accumulated lipid droplets were observed in the uterine tissue of bitches with pyometra. The phosphorylation of AMPK and the expression of PLIN2 significantly increased in the pyometra group. The expression of related lipid synthesis proteins such as ACC1, FASN, SREBP-1c, and PLIN2 was upregulated, while PPARα and PGC1α were downregulated in bitches with pyometra. In experiment two, activation of AMPK and PLIN2 not only restores the expression of PGC1α, but also effectively alleviates inflammation and oxidative stress. The role of lipid metabolism and oxidative stress in canine pyometra is elucidated, thus contributing to the pathogenesis of pyometra in dogs. |
| format | Article |
| id | doaj-art-298bd5c17c37404fbd09ffd54c73e095 |
| institution | DOAJ |
| issn | 1746-6148 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj-art-298bd5c17c37404fbd09ffd54c73e0952025-08-20T03:02:21ZengBMCBMC Veterinary Research1746-61482025-03-0121111210.1186/s12917-025-04622-1Expression of AMPK and PLIN2 in the regulation of lipid metabolism and oxidative stress in bitches with open cervix pyometraXin Deng0Hui Liu1Wei Zhao2Rui Wu3Kuo Chen4Qing Li5Murat Onur Yazlık6Hüseyin Özkan7Jingyuan Ren8Jiacheng Zhang9Shiyi Liu10Ling Mei11Shangfeng Li12Jiasui Zhan13Binhong Hu14College of Chemistry and Life Sciences, Chengdu Normal UniversityDepartment of Pathophysiology, School of Basic Medical Sciences, College of Medicine, Zhengzhou UniversityCollege of Chemistry and Life Sciences, Chengdu Normal UniversityDepartment of Pathophysiology, School of Basic Medical Sciences, College of Medicine, Zhengzhou UniversityThe First Affiliated Hospital of Zhengzhou UniversityCollege of Chemistry and Life Sciences, Chengdu Normal UniversityDepartment of Obstetrics and Gynecology, Ankara University Faculty of Veterinary MedicineFaculty of Veterinary Medicine, Department of Genetics, Hatay Mustafa Kemal UniversityCollege of Chemistry and Life Sciences, Chengdu Normal UniversityThe First Affiliated Hospital of Zhengzhou UniversityCollege of Chemistry and Life Sciences, Chengdu Normal UniversityCollege of Chemistry and Life Sciences, Chengdu Normal UniversityZhi Pet Animal HospitalDepartment of Forest Mycology and Plant Pathology, Swedish University of Agricultural SciencesCollege of Chemistry and Life Sciences, Chengdu Normal UniversityAbstract The pathogenesis of canine pyometra is multifactorial, involving hormonal imbalances, aberrant immune responses, and metabolic dysregulation includes lipid metabolism and oxidative stress. This study focuses on lipid metabolism and oxidative stress, revealing the key regulatory role of AMPK and PLIN2 in canine pyometra. Bitches with open cervix pyometra (n:8) and healthy bitches undergoing elective ovariohysterectomy (n:4) were enrolled to the study. In experiment one, the serum and tissue levels of Malondialdehyde (MDA) and Superoxide Dismutase (SOD) activity were assessed. Additionally, uterine histopathological analysis, AMPK and PLIN2 expressions were determined through immunohistochemistry. Furthermore, inflammation, oxidative stress, and lipid metabolism-related factors were evaluated using Western blot analysis. In experiment two, primary cell cultures were prepared from healthy uterine endometrial cells of the dogs in control group. Cultured canine endometrial epithelial cells were treated with lipopolysaccharide (LPS) along with oleic acid (OA) to induce an inflammatory response. Tissue and serum MDA and SOD levels were greater in dogs with pyometra. Accumulated lipid droplets were observed in the uterine tissue of bitches with pyometra. The phosphorylation of AMPK and the expression of PLIN2 significantly increased in the pyometra group. The expression of related lipid synthesis proteins such as ACC1, FASN, SREBP-1c, and PLIN2 was upregulated, while PPARα and PGC1α were downregulated in bitches with pyometra. In experiment two, activation of AMPK and PLIN2 not only restores the expression of PGC1α, but also effectively alleviates inflammation and oxidative stress. The role of lipid metabolism and oxidative stress in canine pyometra is elucidated, thus contributing to the pathogenesis of pyometra in dogs.https://doi.org/10.1186/s12917-025-04622-1AMPKLipid metabolismOpen cervix pyometraOxidative stressPLIN2 |
| spellingShingle | Xin Deng Hui Liu Wei Zhao Rui Wu Kuo Chen Qing Li Murat Onur Yazlık Hüseyin Özkan Jingyuan Ren Jiacheng Zhang Shiyi Liu Ling Mei Shangfeng Li Jiasui Zhan Binhong Hu Expression of AMPK and PLIN2 in the regulation of lipid metabolism and oxidative stress in bitches with open cervix pyometra BMC Veterinary Research AMPK Lipid metabolism Open cervix pyometra Oxidative stress PLIN2 |
| title | Expression of AMPK and PLIN2 in the regulation of lipid metabolism and oxidative stress in bitches with open cervix pyometra |
| title_full | Expression of AMPK and PLIN2 in the regulation of lipid metabolism and oxidative stress in bitches with open cervix pyometra |
| title_fullStr | Expression of AMPK and PLIN2 in the regulation of lipid metabolism and oxidative stress in bitches with open cervix pyometra |
| title_full_unstemmed | Expression of AMPK and PLIN2 in the regulation of lipid metabolism and oxidative stress in bitches with open cervix pyometra |
| title_short | Expression of AMPK and PLIN2 in the regulation of lipid metabolism and oxidative stress in bitches with open cervix pyometra |
| title_sort | expression of ampk and plin2 in the regulation of lipid metabolism and oxidative stress in bitches with open cervix pyometra |
| topic | AMPK Lipid metabolism Open cervix pyometra Oxidative stress PLIN2 |
| url | https://doi.org/10.1186/s12917-025-04622-1 |
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