The Hydroalcoholic Extract Obtained from Mentha piperita L. Leaves Attenuates Oxidative Stress and Improves Survival in Lipopolysaccharide-Treated Macrophages
Mentha piperita L. (peppermint) possesses antimicrobial properties, but little is known of its ability to modulate macrophages. Macrophages are essential in bacterial infection control due to their antimicrobial functions and ability to link the innate and adaptive immune responses. We evaluated the...
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Wiley
2017-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/2078794 |
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| author | Mariana Oliveira Arruda Saulo José Figueiredo Mendes Simone Aparecida Teixeira Ludmilla Santos Silva de Mesquita Maria Nilce de Sousa Ribeiro Stanley de Sousa Lima Galvão Marcelo Nicolás Muscará Elizabeth Soares Fernandes Valério Monteiro-Neto |
| author_facet | Mariana Oliveira Arruda Saulo José Figueiredo Mendes Simone Aparecida Teixeira Ludmilla Santos Silva de Mesquita Maria Nilce de Sousa Ribeiro Stanley de Sousa Lima Galvão Marcelo Nicolás Muscará Elizabeth Soares Fernandes Valério Monteiro-Neto |
| author_sort | Mariana Oliveira Arruda |
| collection | DOAJ |
| description | Mentha piperita L. (peppermint) possesses antimicrobial properties, but little is known of its ability to modulate macrophages. Macrophages are essential in bacterial infection control due to their antimicrobial functions and ability to link the innate and adaptive immune responses. We evaluated the effects of the peppermint leaf hydroalcoholic extract (LHAE) on cultured murine peritoneal macrophages stimulated or not with lipopolysaccharide (LPS) in vitro. Vehicle-treated cells were used as controls. The constituents of the extract were also identified. Epicatechin was the major compound detected in the LHAE. LPS-induced macrophage death was reversed by incubation with LHAE (1–30 μg/ml). Higher concentrations of the extract (≥100 μg/ml) decreased macrophage viability (49–57%) in the absence of LPS. LHAE (1–300 μg/ml) attenuated H2O2 (34.6–53.4%) but not nitric oxide production by these cells. At similar concentrations, the extract increased the activity of superoxide dismutase (15.3–63.5-fold) and glutathione peroxidase (34.4–73.6-fold) in LPS-treated macrophages. Only LPS-unstimulated macrophages presented enhanced phagocytosis (3.6–6.6-fold increase) when incubated with LHAE (3–30 μg/ml). Overall, the LHAE obtained from peppermint modulates macrophage-mediated inflammatory responses, by stimulating the antioxidant pathway in these cells. These effects may be beneficial when the excessive activation of macrophages contributes to tissue damage during infectious disease. |
| format | Article |
| id | doaj-art-298a599ab0ae4b7787d05efd422f6a81 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-298a599ab0ae4b7787d05efd422f6a812025-02-03T01:11:07ZengWileyJournal of Immunology Research2314-88612314-71562017-01-01201710.1155/2017/20787942078794The Hydroalcoholic Extract Obtained from Mentha piperita L. Leaves Attenuates Oxidative Stress and Improves Survival in Lipopolysaccharide-Treated MacrophagesMariana Oliveira Arruda0Saulo José Figueiredo Mendes1Simone Aparecida Teixeira2Ludmilla Santos Silva de Mesquita3Maria Nilce de Sousa Ribeiro4Stanley de Sousa Lima Galvão5Marcelo Nicolás Muscará6Elizabeth Soares Fernandes7Valério Monteiro-Neto8Programa de Pós-graduação, Universidade CEUMA, Rua Josué Montello, No. 1, Renascença, 65075-120 São Luís, MA, BrazilPrograma de Pós-graduação, Universidade CEUMA, Rua Josué Montello, No. 1, Renascença, 65075-120 São Luís, MA, BrazilDepartamento de Farmacologia, Universidade de São Paulo, Av. Prof. Lineu Prestes, No. 1524 Sala 326, Butantan, 05508-900 São Paulo, SP, BrazilLaboratório de Farmacognosia, 65080-805 São Luís, MA, BrazilLaboratório de Farmacognosia, 65080-805 São Luís, MA, BrazilPrograma de Pós-graduação, Universidade CEUMA, Rua Josué Montello, No. 1, Renascença, 65075-120 São Luís, MA, BrazilDepartamento de Farmacologia, Universidade de São Paulo, Av. Prof. Lineu Prestes, No. 1524 Sala 326, Butantan, 05508-900 São Paulo, SP, BrazilPrograma de Pós-graduação, Universidade CEUMA, Rua Josué Montello, No. 1, Renascença, 65075-120 São Luís, MA, BrazilPrograma de Pós-graduação, Universidade CEUMA, Rua Josué Montello, No. 1, Renascença, 65075-120 São Luís, MA, BrazilMentha piperita L. (peppermint) possesses antimicrobial properties, but little is known of its ability to modulate macrophages. Macrophages are essential in bacterial infection control due to their antimicrobial functions and ability to link the innate and adaptive immune responses. We evaluated the effects of the peppermint leaf hydroalcoholic extract (LHAE) on cultured murine peritoneal macrophages stimulated or not with lipopolysaccharide (LPS) in vitro. Vehicle-treated cells were used as controls. The constituents of the extract were also identified. Epicatechin was the major compound detected in the LHAE. LPS-induced macrophage death was reversed by incubation with LHAE (1–30 μg/ml). Higher concentrations of the extract (≥100 μg/ml) decreased macrophage viability (49–57%) in the absence of LPS. LHAE (1–300 μg/ml) attenuated H2O2 (34.6–53.4%) but not nitric oxide production by these cells. At similar concentrations, the extract increased the activity of superoxide dismutase (15.3–63.5-fold) and glutathione peroxidase (34.4–73.6-fold) in LPS-treated macrophages. Only LPS-unstimulated macrophages presented enhanced phagocytosis (3.6–6.6-fold increase) when incubated with LHAE (3–30 μg/ml). Overall, the LHAE obtained from peppermint modulates macrophage-mediated inflammatory responses, by stimulating the antioxidant pathway in these cells. These effects may be beneficial when the excessive activation of macrophages contributes to tissue damage during infectious disease.http://dx.doi.org/10.1155/2017/2078794 |
| spellingShingle | Mariana Oliveira Arruda Saulo José Figueiredo Mendes Simone Aparecida Teixeira Ludmilla Santos Silva de Mesquita Maria Nilce de Sousa Ribeiro Stanley de Sousa Lima Galvão Marcelo Nicolás Muscará Elizabeth Soares Fernandes Valério Monteiro-Neto The Hydroalcoholic Extract Obtained from Mentha piperita L. Leaves Attenuates Oxidative Stress and Improves Survival in Lipopolysaccharide-Treated Macrophages Journal of Immunology Research |
| title | The Hydroalcoholic Extract Obtained from Mentha piperita L. Leaves Attenuates Oxidative Stress and Improves Survival in Lipopolysaccharide-Treated Macrophages |
| title_full | The Hydroalcoholic Extract Obtained from Mentha piperita L. Leaves Attenuates Oxidative Stress and Improves Survival in Lipopolysaccharide-Treated Macrophages |
| title_fullStr | The Hydroalcoholic Extract Obtained from Mentha piperita L. Leaves Attenuates Oxidative Stress and Improves Survival in Lipopolysaccharide-Treated Macrophages |
| title_full_unstemmed | The Hydroalcoholic Extract Obtained from Mentha piperita L. Leaves Attenuates Oxidative Stress and Improves Survival in Lipopolysaccharide-Treated Macrophages |
| title_short | The Hydroalcoholic Extract Obtained from Mentha piperita L. Leaves Attenuates Oxidative Stress and Improves Survival in Lipopolysaccharide-Treated Macrophages |
| title_sort | hydroalcoholic extract obtained from mentha piperita l leaves attenuates oxidative stress and improves survival in lipopolysaccharide treated macrophages |
| url | http://dx.doi.org/10.1155/2017/2078794 |
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