Genetic Diversity of Melipona mandacaia SMITH 1863 (Hymenoptera, Apidae), an Endemic Bee Species from Brazilian Caatinga, Using ISSR
In order to evaluate the genetic diversity and structure of Melipona mandacaia, we analyzed 104 colonies collected in 12 localities in Bahia state, northeastern Brazil, using ISSR-PCR. A total of 109 bands were obtained with a significant polymorphism of 72.47%. Estimates of genetic diversity indica...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Psyche: A Journal of Entomology |
| Online Access: | http://dx.doi.org/10.1155/2012/372138 |
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| author | Elder Assis Miranda Henrique Batalha-Filho Priscila Santos Oliveira Rogério Marcos Oliveira Alves Lucio Antonio Oliveira Campos Ana Maria Waldschmidt |
| author_facet | Elder Assis Miranda Henrique Batalha-Filho Priscila Santos Oliveira Rogério Marcos Oliveira Alves Lucio Antonio Oliveira Campos Ana Maria Waldschmidt |
| author_sort | Elder Assis Miranda |
| collection | DOAJ |
| description | In order to evaluate the genetic diversity and structure of Melipona mandacaia, we analyzed 104 colonies collected in 12 localities in Bahia state, northeastern Brazil, using ISSR-PCR. A total of 109 bands were obtained with a significant polymorphism of 72.47%. Estimates of genetic diversity indicated low values of heterozygosity (He and HB values were 0.2616 and 0.2573, resp.). These reduced values have been reported in other studies in stingless bees and maybe justified by dispersion process in the origin of new nests. AMOVA revealed that the higher percentage of variation is within localities (70.39%). The ΦST and θB values were, respectively, 0.2961 and 0.3289, thereby indicating a moderate population structuring. The correlation between genetic and geographic distances (r=0.4542; P<0.01) suggests isolation by distance. Our study contributes to describing the genetic diversity of endemic organisms from Caatinga and may help future efforts to preserve this threatened biome. |
| format | Article |
| id | doaj-art-297ab7e1aeb54fa194ba233372b76452 |
| institution | Kabale University |
| issn | 0033-2615 1687-7438 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Psyche: A Journal of Entomology |
| spelling | doaj-art-297ab7e1aeb54fa194ba233372b764522025-02-03T01:01:59ZengWileyPsyche: A Journal of Entomology0033-26151687-74382012-01-01201210.1155/2012/372138372138Genetic Diversity of Melipona mandacaia SMITH 1863 (Hymenoptera, Apidae), an Endemic Bee Species from Brazilian Caatinga, Using ISSRElder Assis Miranda0Henrique Batalha-Filho1Priscila Santos Oliveira2Rogério Marcos Oliveira Alves3Lucio Antonio Oliveira Campos4Ana Maria Waldschmidt5Departamento de Ciências Biológicas, Universidade Estadual do Sudoeste da Bahia, Avenida José Moreira Sobrinho s/n, 45206-190 Jequié, BA, BrazilDepartamento de Genética e Biologia Evolutiva, Instituto de Biociências, USP, Rua do Matão 277, Cidade Universitária, 05508-090 São Paulo, SP, BrazilDepartamento de Ciências Biológicas, Universidade Estadual do Sudoeste da Bahia, Avenida José Moreira Sobrinho s/n, 45206-190 Jequié, BA, BrazilEscola Agrotécnica Federal de Catu, Rua Barão de Camacari 118, 48110-000 Catu, BA, BrazilDepartamento de Biologia Geral, Universidade Federal de Viçosa, Avenida Peter Henry Rolfs s/n Campus Universitário, 36571-000 Viçosa, MG, BrazilDepartamento de Ciências Biológicas, Universidade Estadual do Sudoeste da Bahia, Avenida José Moreira Sobrinho s/n, 45206-190 Jequié, BA, BrazilIn order to evaluate the genetic diversity and structure of Melipona mandacaia, we analyzed 104 colonies collected in 12 localities in Bahia state, northeastern Brazil, using ISSR-PCR. A total of 109 bands were obtained with a significant polymorphism of 72.47%. Estimates of genetic diversity indicated low values of heterozygosity (He and HB values were 0.2616 and 0.2573, resp.). These reduced values have been reported in other studies in stingless bees and maybe justified by dispersion process in the origin of new nests. AMOVA revealed that the higher percentage of variation is within localities (70.39%). The ΦST and θB values were, respectively, 0.2961 and 0.3289, thereby indicating a moderate population structuring. The correlation between genetic and geographic distances (r=0.4542; P<0.01) suggests isolation by distance. Our study contributes to describing the genetic diversity of endemic organisms from Caatinga and may help future efforts to preserve this threatened biome.http://dx.doi.org/10.1155/2012/372138 |
| spellingShingle | Elder Assis Miranda Henrique Batalha-Filho Priscila Santos Oliveira Rogério Marcos Oliveira Alves Lucio Antonio Oliveira Campos Ana Maria Waldschmidt Genetic Diversity of Melipona mandacaia SMITH 1863 (Hymenoptera, Apidae), an Endemic Bee Species from Brazilian Caatinga, Using ISSR Psyche: A Journal of Entomology |
| title | Genetic Diversity of Melipona mandacaia SMITH 1863 (Hymenoptera, Apidae), an Endemic Bee Species from Brazilian Caatinga, Using ISSR |
| title_full | Genetic Diversity of Melipona mandacaia SMITH 1863 (Hymenoptera, Apidae), an Endemic Bee Species from Brazilian Caatinga, Using ISSR |
| title_fullStr | Genetic Diversity of Melipona mandacaia SMITH 1863 (Hymenoptera, Apidae), an Endemic Bee Species from Brazilian Caatinga, Using ISSR |
| title_full_unstemmed | Genetic Diversity of Melipona mandacaia SMITH 1863 (Hymenoptera, Apidae), an Endemic Bee Species from Brazilian Caatinga, Using ISSR |
| title_short | Genetic Diversity of Melipona mandacaia SMITH 1863 (Hymenoptera, Apidae), an Endemic Bee Species from Brazilian Caatinga, Using ISSR |
| title_sort | genetic diversity of melipona mandacaia smith 1863 hymenoptera apidae an endemic bee species from brazilian caatinga using issr |
| url | http://dx.doi.org/10.1155/2012/372138 |
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