Expression of 9-<i>O</i>-Acetylated Sialic Acid in HPV+ Oral Squamous Cell Carcinoma Cells
Oral squamous cell carcinoma (OSCC) is a common type of head and neck malignancy that represents a significant global health issue. Sialylations are common events in tumor transformation, proliferation, metastasis, and immune evasion. Modifications in sialylation can be detected by lectins, whose ch...
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2025-04-01
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| author | Hugo Sánchez-Martínez Victoria Jiménez-Castillo Daniela Illescas-Barbosa Beatriz Xochitl Ávila-Curiel María Teresa Hernández-Huerta Risk Díaz-Castillejos Rafael Torres-Rosas Edgar Zenteno Mohamed Alí Pereyra-Morales Carlos Josué Solórzano-Mata |
| author_facet | Hugo Sánchez-Martínez Victoria Jiménez-Castillo Daniela Illescas-Barbosa Beatriz Xochitl Ávila-Curiel María Teresa Hernández-Huerta Risk Díaz-Castillejos Rafael Torres-Rosas Edgar Zenteno Mohamed Alí Pereyra-Morales Carlos Josué Solórzano-Mata |
| author_sort | Hugo Sánchez-Martínez |
| collection | DOAJ |
| description | Oral squamous cell carcinoma (OSCC) is a common type of head and neck malignancy that represents a significant global health issue. Sialylations are common events in tumor transformation, proliferation, metastasis, and immune evasion. Modifications in sialylation can be detected by lectins, whose changes in OSCC have been related to grade, invasion, and metastasis. The presence of 9-<i>O</i>-acetylated sialic acid (Neu5,9Ac<sub>2</sub>) in OSCC cells and its potential expression, modification, and role are unknown. This study aimed to analyze the expression of Neu5,9Ac<sub>2</sub> using the <i>Macrobrachium rosenbergii</i> lectin (MrL) that recognizes this sialic acid (Neu5Ac) residue and also compare its effect on the SCC-152 cell line (CRL-3240, ATCC) and immortalized keratinocytes (HaCaT) as a control. We observed by immunocytochemistry that SCC-152 cells expressed more Neu5,9Ac<sub>2</sub> compared to HaCaT cells; the specificity of MrL was confirmed after the sialidase treatment of cells in which the loss of lectin’s recognition of Neu5,9Ac<sub>2</sub> was observed. The electrophoretic profile was similar between both cell line types; however, the Western blot showed differences in the glycoprotein patterns recognized by lectin for each cell type. MrL increased the proliferation of SCC-152 cells, as well as the integrity and morphology of the colonies. Therefore, our results suggest that Neu5,9Ac<sub>2</sub> glycosylated receptors could be involved in the survival and proliferation of OSCC cells, which offers a promising avenue for developing diagnostic and prognostic tools (tumor markers) against oral squamous cell carcinoma in the future. |
| format | Article |
| id | doaj-art-29771b34ceee40cda75201f9cef21f00 |
| institution | DOAJ |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-04-01 |
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| spelling | doaj-art-29771b34ceee40cda75201f9cef21f002025-08-20T03:13:52ZengMDPI AGLife2075-17292025-04-0115466310.3390/life15040663Expression of 9-<i>O</i>-Acetylated Sialic Acid in HPV+ Oral Squamous Cell Carcinoma CellsHugo Sánchez-Martínez0Victoria Jiménez-Castillo1Daniela Illescas-Barbosa2Beatriz Xochitl Ávila-Curiel3María Teresa Hernández-Huerta4Risk Díaz-Castillejos5Rafael Torres-Rosas6Edgar Zenteno7Mohamed Alí Pereyra-Morales8Carlos Josué Solórzano-Mata9Faculty of Dentistry, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca 68120, MexicoFaculty of Medicine and Surgery, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca 68120, MexicoFaculty of Dentistry, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca 68120, MexicoFaculty of Dentistry, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca 68120, MexicoSECIHTI-Faculty of Medicine and Surgery, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca 68120, MexicoFaculty of Dentistry, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca 68120, MexicoFaculty of Dentistry, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca 68120, MexicoDepartament of Biochemistry, Faculty of Medicine, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoDepartament of Biochemistry, Faculty of Medicine, Universidad Nacional Autónoma de México, Ciudad de México 04510, MexicoFaculty of Dentistry, Universidad Autónoma Benito Juárez de Oaxaca, Oaxaca 68120, MexicoOral squamous cell carcinoma (OSCC) is a common type of head and neck malignancy that represents a significant global health issue. Sialylations are common events in tumor transformation, proliferation, metastasis, and immune evasion. Modifications in sialylation can be detected by lectins, whose changes in OSCC have been related to grade, invasion, and metastasis. The presence of 9-<i>O</i>-acetylated sialic acid (Neu5,9Ac<sub>2</sub>) in OSCC cells and its potential expression, modification, and role are unknown. This study aimed to analyze the expression of Neu5,9Ac<sub>2</sub> using the <i>Macrobrachium rosenbergii</i> lectin (MrL) that recognizes this sialic acid (Neu5Ac) residue and also compare its effect on the SCC-152 cell line (CRL-3240, ATCC) and immortalized keratinocytes (HaCaT) as a control. We observed by immunocytochemistry that SCC-152 cells expressed more Neu5,9Ac<sub>2</sub> compared to HaCaT cells; the specificity of MrL was confirmed after the sialidase treatment of cells in which the loss of lectin’s recognition of Neu5,9Ac<sub>2</sub> was observed. The electrophoretic profile was similar between both cell line types; however, the Western blot showed differences in the glycoprotein patterns recognized by lectin for each cell type. MrL increased the proliferation of SCC-152 cells, as well as the integrity and morphology of the colonies. Therefore, our results suggest that Neu5,9Ac<sub>2</sub> glycosylated receptors could be involved in the survival and proliferation of OSCC cells, which offers a promising avenue for developing diagnostic and prognostic tools (tumor markers) against oral squamous cell carcinoma in the future.https://www.mdpi.com/2075-1729/15/4/663OSCCNeu5,9Ac<sub>2</sub>Neu5Ac<i>Macrobrachium rosenbergii</i> lectinSCC-152HaCaT |
| spellingShingle | Hugo Sánchez-Martínez Victoria Jiménez-Castillo Daniela Illescas-Barbosa Beatriz Xochitl Ávila-Curiel María Teresa Hernández-Huerta Risk Díaz-Castillejos Rafael Torres-Rosas Edgar Zenteno Mohamed Alí Pereyra-Morales Carlos Josué Solórzano-Mata Expression of 9-<i>O</i>-Acetylated Sialic Acid in HPV+ Oral Squamous Cell Carcinoma Cells Life OSCC Neu5,9Ac<sub>2</sub> Neu5Ac <i>Macrobrachium rosenbergii</i> lectin SCC-152 HaCaT |
| title | Expression of 9-<i>O</i>-Acetylated Sialic Acid in HPV+ Oral Squamous Cell Carcinoma Cells |
| title_full | Expression of 9-<i>O</i>-Acetylated Sialic Acid in HPV+ Oral Squamous Cell Carcinoma Cells |
| title_fullStr | Expression of 9-<i>O</i>-Acetylated Sialic Acid in HPV+ Oral Squamous Cell Carcinoma Cells |
| title_full_unstemmed | Expression of 9-<i>O</i>-Acetylated Sialic Acid in HPV+ Oral Squamous Cell Carcinoma Cells |
| title_short | Expression of 9-<i>O</i>-Acetylated Sialic Acid in HPV+ Oral Squamous Cell Carcinoma Cells |
| title_sort | expression of 9 i o i acetylated sialic acid in hpv oral squamous cell carcinoma cells |
| topic | OSCC Neu5,9Ac<sub>2</sub> Neu5Ac <i>Macrobrachium rosenbergii</i> lectin SCC-152 HaCaT |
| url | https://www.mdpi.com/2075-1729/15/4/663 |
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