Raising Awareness of Canine, Feline and Human Dirofilariosis in Aveiro, Portugal: A One Health Perspective

Climatic and various socio-geographical variables have significantly influenced the global spread of <i>Dirofilaria immitis</i>. The coastal district of Aveiro, Portugal, marked by its unique hydrographic structure, Ria de Aveiro, and a concerning rise in heartworm disease, was the focus...

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Main Authors: Joana Esteves-Guimarães, José Alberto Montoya-Alonso, Jorge Isidoro Matos, Elmano Ramalheira, Elena Carretón, Ivan Rodríguez-Escolar, Alfonso Balmori-de la Puente, Manuel Collado-Cuadrado, Rodrigo Morchón, Ana Patrícia Fontes-Sousa
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/7/952
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author Joana Esteves-Guimarães
José Alberto Montoya-Alonso
Jorge Isidoro Matos
Elmano Ramalheira
Elena Carretón
Ivan Rodríguez-Escolar
Alfonso Balmori-de la Puente
Manuel Collado-Cuadrado
Rodrigo Morchón
Ana Patrícia Fontes-Sousa
author_facet Joana Esteves-Guimarães
José Alberto Montoya-Alonso
Jorge Isidoro Matos
Elmano Ramalheira
Elena Carretón
Ivan Rodríguez-Escolar
Alfonso Balmori-de la Puente
Manuel Collado-Cuadrado
Rodrigo Morchón
Ana Patrícia Fontes-Sousa
author_sort Joana Esteves-Guimarães
collection DOAJ
description Climatic and various socio-geographical variables have significantly influenced the global spread of <i>Dirofilaria immitis</i>. The coastal district of Aveiro, Portugal, marked by its unique hydrographic structure, Ria de Aveiro, and a concerning rise in heartworm disease, was the focus of our study. We aimed to update the prevalence of <i>D. immitis</i> in dogs and the seroprevalence in cats and humans, correlating these data with epidemiological information. A total of 430 dogs were sampled for <i>D. immitis</i> antigens, and 426 cats and 398 humans for <i>D. immitis</i> and <i>Wolbachia</i> sp. antibodies. In addition, we developed and validated an infection risk map for <i>D.</i> immitis with the geolocation of positive samples. Our results indicate a canine prevalence of 4.7%, peaking at 16.7% in Vagos. Feline and human seroprevalences were 8.9% (26.7% in Espinho) and 3.0% (Vagos presented the most alarming results), respectively. Positive samples were found in both high- and low-risk areas highlighting the need for chemoprophylaxis in all municipalities. Risk factors identified included lack of vaccination and internal deworming in cats, while dogs faced risks from inadequate vaccination and outdoor exposure. Our study identifies Aveiro as an endemic area, with a need for control measures to address this public health threat.
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spelling doaj-art-29620dd0220942df8d162e46d7c9e8d72025-08-20T03:06:32ZengMDPI AGAnimals2076-26152025-03-0115795210.3390/ani15070952Raising Awareness of Canine, Feline and Human Dirofilariosis in Aveiro, Portugal: A One Health PerspectiveJoana Esteves-Guimarães0José Alberto Montoya-Alonso1Jorge Isidoro Matos2Elmano Ramalheira3Elena Carretón4Ivan Rodríguez-Escolar5Alfonso Balmori-de la Puente6Manuel Collado-Cuadrado7Rodrigo Morchón8Ana Patrícia Fontes-Sousa9Clínica Veterinária Aanifeira, 4520-409 Santa Maria da Feira, PortugalInternal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, SpainInternal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, SpainClinical Pathology Department, Baixo Vouga-Public Business Entity (EPE) Hospital Centre, Avenida Artur Ravara, 3810-501 Aveiro, PortugalInternal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, SpainZoonotic Infections and One Health Group, Biomedical Research Institute of Salamanca (IBSAL), Centre for Environmental Studies and Rural Dynamization (CEADIR), Faculty of Pharmacy, University of Salamanca, Campus Miguel Unamuno, 37007 Salamanca, SpainZoonotic Infections and One Health Group, Biomedical Research Institute of Salamanca (IBSAL), Centre for Environmental Studies and Rural Dynamization (CEADIR), Faculty of Pharmacy, University of Salamanca, Campus Miguel Unamuno, 37007 Salamanca, SpainZoonotic Infections and One Health Group, Biomedical Research Institute of Salamanca (IBSAL), Centre for Environmental Studies and Rural Dynamization (CEADIR), Faculty of Pharmacy, University of Salamanca, Campus Miguel Unamuno, 37007 Salamanca, SpainInternal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, SpainInternal Medicine, Faculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, 35413 Las Palmas de Gran Canaria, SpainClimatic and various socio-geographical variables have significantly influenced the global spread of <i>Dirofilaria immitis</i>. The coastal district of Aveiro, Portugal, marked by its unique hydrographic structure, Ria de Aveiro, and a concerning rise in heartworm disease, was the focus of our study. We aimed to update the prevalence of <i>D. immitis</i> in dogs and the seroprevalence in cats and humans, correlating these data with epidemiological information. A total of 430 dogs were sampled for <i>D. immitis</i> antigens, and 426 cats and 398 humans for <i>D. immitis</i> and <i>Wolbachia</i> sp. antibodies. In addition, we developed and validated an infection risk map for <i>D.</i> immitis with the geolocation of positive samples. Our results indicate a canine prevalence of 4.7%, peaking at 16.7% in Vagos. Feline and human seroprevalences were 8.9% (26.7% in Espinho) and 3.0% (Vagos presented the most alarming results), respectively. Positive samples were found in both high- and low-risk areas highlighting the need for chemoprophylaxis in all municipalities. Risk factors identified included lack of vaccination and internal deworming in cats, while dogs faced risks from inadequate vaccination and outdoor exposure. Our study identifies Aveiro as an endemic area, with a need for control measures to address this public health threat.https://www.mdpi.com/2076-2615/15/7/952<i>Dirofilaria immitis</i><i>Wolbachia pipientis</i>zoonosisAveiroPortugalseroepidemiological study
spellingShingle Joana Esteves-Guimarães
José Alberto Montoya-Alonso
Jorge Isidoro Matos
Elmano Ramalheira
Elena Carretón
Ivan Rodríguez-Escolar
Alfonso Balmori-de la Puente
Manuel Collado-Cuadrado
Rodrigo Morchón
Ana Patrícia Fontes-Sousa
Raising Awareness of Canine, Feline and Human Dirofilariosis in Aveiro, Portugal: A One Health Perspective
Animals
<i>Dirofilaria immitis</i>
<i>Wolbachia pipientis</i>
zoonosis
Aveiro
Portugal
seroepidemiological study
title Raising Awareness of Canine, Feline and Human Dirofilariosis in Aveiro, Portugal: A One Health Perspective
title_full Raising Awareness of Canine, Feline and Human Dirofilariosis in Aveiro, Portugal: A One Health Perspective
title_fullStr Raising Awareness of Canine, Feline and Human Dirofilariosis in Aveiro, Portugal: A One Health Perspective
title_full_unstemmed Raising Awareness of Canine, Feline and Human Dirofilariosis in Aveiro, Portugal: A One Health Perspective
title_short Raising Awareness of Canine, Feline and Human Dirofilariosis in Aveiro, Portugal: A One Health Perspective
title_sort raising awareness of canine feline and human dirofilariosis in aveiro portugal a one health perspective
topic <i>Dirofilaria immitis</i>
<i>Wolbachia pipientis</i>
zoonosis
Aveiro
Portugal
seroepidemiological study
url https://www.mdpi.com/2076-2615/15/7/952
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