Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats
Jie Lv, Wei Ou, Xiao-Hua Zou, Yin Yao, Jin-Li Wu Department of Anesthesia, Guizhou Medical University Affiliated Hospital, Guiyang, People’s Republic of China Abstract: The study aimed to explore the effect of dexmedetomidine (DEX) on hippocampal neuron development process and on mol...
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Dove Medical Press
2016-12-01
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| Series: | Neuropsychiatric Disease and Treatment |
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| Online Access: | https://www.dovepress.com/effect-of-dexmedetomidine-on-hippocampal-neuron-development-and-bdnf-t-peer-reviewed-fulltext-article-NDT |
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| author | Lv J Ou W Zou XH Yao Y Wu JL |
| author_facet | Lv J Ou W Zou XH Yao Y Wu JL |
| author_sort | Lv J |
| collection | DOAJ |
| description | Jie Lv, Wei Ou, Xiao-Hua Zou, Yin Yao, Jin-Li Wu Department of Anesthesia, Guizhou Medical University Affiliated Hospital, Guiyang, People’s Republic of China Abstract: The study aimed to explore the effect of dexmedetomidine (DEX) on hippocampal neuron development process and on molecular expression of brain-derived neurotrophic factor (BDNF)-tyrosine receptor kinase B (TrkB) signaling pathway in neonatal rats. The hippocampal neuron cells were isolated from newborn neonatal rats and cultured in vitro. One control group and three treated groups with 1, 10, and 100 µmol/L DEX were used for the study. Cell activity and apoptosis were detected by the MTT and terminal deoxynucleotidyl transferase-mediated biotinylated uridine triphosphate (UTP) nick end labeling assays. The synaptophysin (SYN) and postsynaptic density 95 (PSD95) were detected by quantitative polymerase chain reaction. There was no difference in the viability of neuron cells among the different dose groups of DEX and the control group during days 2-10 (P>0.05). Compared to the control group, there was no significant difference (P>0.05) in the expressions of SYN and PSD95 in the groups treated with 1 and 10 µmol/L DEX, whereas significant difference in the expression was observed in the group treated with 100 µmol/L DEX (P<0.01). Compared with the control group, the expression of BDNF was significantly upregulated (P<0.05) in the group treated with 100 µmol/L DEX. There were no significant differences in TrkB expression among the four groups. The expression of p-N-methyl-D-aspartate receptor increased with an increase in the concentration of DEX; however, only the high dose revealed a significant upregulation compared with the control group. The neuroprotective effect of DEX may be achieved by upregulating the expression of BDNF and phosphorylation level of N-methyl-D-aspartate receptor. Keywords: dexmedetomidine, hippocampal neuron, development, BDNF-TrkB |
| format | Article |
| id | doaj-art-29618105cf274e7ea3a850b4f52cb0b4 |
| institution | Kabale University |
| issn | 1178-2021 |
| language | English |
| publishDate | 2016-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Neuropsychiatric Disease and Treatment |
| spelling | doaj-art-29618105cf274e7ea3a850b4f52cb0b42025-08-20T03:27:01ZengDove Medical PressNeuropsychiatric Disease and Treatment1178-20212016-12-01Volume 12Issue 13153315930424Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal ratsLv JOu W0Zou XHYao YWu JLDepartment of AnesthesiaJie Lv, Wei Ou, Xiao-Hua Zou, Yin Yao, Jin-Li Wu Department of Anesthesia, Guizhou Medical University Affiliated Hospital, Guiyang, People’s Republic of China Abstract: The study aimed to explore the effect of dexmedetomidine (DEX) on hippocampal neuron development process and on molecular expression of brain-derived neurotrophic factor (BDNF)-tyrosine receptor kinase B (TrkB) signaling pathway in neonatal rats. The hippocampal neuron cells were isolated from newborn neonatal rats and cultured in vitro. One control group and three treated groups with 1, 10, and 100 µmol/L DEX were used for the study. Cell activity and apoptosis were detected by the MTT and terminal deoxynucleotidyl transferase-mediated biotinylated uridine triphosphate (UTP) nick end labeling assays. The synaptophysin (SYN) and postsynaptic density 95 (PSD95) were detected by quantitative polymerase chain reaction. There was no difference in the viability of neuron cells among the different dose groups of DEX and the control group during days 2-10 (P>0.05). Compared to the control group, there was no significant difference (P>0.05) in the expressions of SYN and PSD95 in the groups treated with 1 and 10 µmol/L DEX, whereas significant difference in the expression was observed in the group treated with 100 µmol/L DEX (P<0.01). Compared with the control group, the expression of BDNF was significantly upregulated (P<0.05) in the group treated with 100 µmol/L DEX. There were no significant differences in TrkB expression among the four groups. The expression of p-N-methyl-D-aspartate receptor increased with an increase in the concentration of DEX; however, only the high dose revealed a significant upregulation compared with the control group. The neuroprotective effect of DEX may be achieved by upregulating the expression of BDNF and phosphorylation level of N-methyl-D-aspartate receptor. Keywords: dexmedetomidine, hippocampal neuron, development, BDNF-TrkBhttps://www.dovepress.com/effect-of-dexmedetomidine-on-hippocampal-neuron-development-and-bdnf-t-peer-reviewed-fulltext-article-NDTdexmedetomidinehippocampal neurondevelopmentBDNF-TrkB |
| spellingShingle | Lv J Ou W Zou XH Yao Y Wu JL Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats Neuropsychiatric Disease and Treatment dexmedetomidine hippocampal neuron development BDNF-TrkB |
| title | Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats |
| title_full | Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats |
| title_fullStr | Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats |
| title_full_unstemmed | Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats |
| title_short | Effect of dexmedetomidine on hippocampal neuron development and BDNF-TrkB signal expression in neonatal rats |
| title_sort | effect of dexmedetomidine on hippocampal neuron development and bdnf trkb signal expression in neonatal rats |
| topic | dexmedetomidine hippocampal neuron development BDNF-TrkB |
| url | https://www.dovepress.com/effect-of-dexmedetomidine-on-hippocampal-neuron-development-and-bdnf-t-peer-reviewed-fulltext-article-NDT |
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