Gum Arabic-assisted green synthesis of gold nanoparticles as fluorescence modulator for potential analytical applications
Aim: To demonstrate a simple, eco-friendly, and cost-effective green method to synthesize gold nanoparticles (AuNPs) using the aqueous extract of gum Arabic (GA) as a reducing and stabilizing agent. Methods: Green synthesis of nanoparticles, characterization by absorption, infra-red and fluorescence...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Open Exploration Publishing Inc.
2024-07-01
|
| Series: | Exploration of BioMat-X |
| Subjects: | |
| Online Access: | https://www.explorationpub.com/uploads/Article/A101314/101314.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849717842454249472 |
|---|---|
| author | Ahmed T. Algahiny Omar S. Elmitwalli Deyari A. Kassim G. Roshan Deen Sultan Akhtar Fryad Z. Henari |
| author_facet | Ahmed T. Algahiny Omar S. Elmitwalli Deyari A. Kassim G. Roshan Deen Sultan Akhtar Fryad Z. Henari |
| author_sort | Ahmed T. Algahiny |
| collection | DOAJ |
| description | Aim: To demonstrate a simple, eco-friendly, and cost-effective green method to synthesize gold nanoparticles (AuNPs) using the aqueous extract of gum Arabic (GA) as a reducing and stabilizing agent. Methods: Green synthesis of nanoparticles, characterization by absorption, infra-red and fluorescence spectroscopy. Results: The absorption spectrum (UV-Vis) showed an absorption peak ~522 nm corresponding to the surface plasmon resonance (SPR) absorption peak of AuNPs. Transmission electron microscopy (TEM) images revealed spherical-shaped nanoparticles with an average size of 15 nm. Fourier transform infrared (FTIR) analysis showed that the nanoparticles are coated with organic compounds that are present in GA. The fluorescence quenching properties of the AuNPs were assessed by monitoring their effects on fluorescence intensity of coumarin 153 (C153) dye. The fluorescence of the dye decreased with an increase in concentration of the nanoparticles. Upon addition of the protein bovine serum albumin (BSA) to the mixture the fluorescence increased (recovery) again. Conclusions: The fluorescence quenching and recovery (turn-on/off system) is a valuable method for protein detection in solution. By observing the effect of BSA on the quenched fluorescence, this nanoparticle system shows promise in biomedicine, drug delivery and environmental monitoring. |
| format | Article |
| id | doaj-art-295bb7e0465247dfa4511fec7b1d8bca |
| institution | DOAJ |
| issn | 2996-9476 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Open Exploration Publishing Inc. |
| record_format | Article |
| series | Exploration of BioMat-X |
| spelling | doaj-art-295bb7e0465247dfa4511fec7b1d8bca2025-08-20T03:12:32ZengOpen Exploration Publishing Inc.Exploration of BioMat-X2996-94762024-07-011419020110.37349/ebmx.2024.00014Gum Arabic-assisted green synthesis of gold nanoparticles as fluorescence modulator for potential analytical applicationsAhmed T. Algahiny0https://orcid.org/0009-0007-3767-4745Omar S. Elmitwalli1https://orcid.org/0000-0003-1356-4473Deyari A. Kassim2https://orcid.org/0009-0002-0978-1694G. Roshan Deen3https://orcid.org/0000-0001-8733-0843Sultan Akhtar4https://orcid.org/0000-0002-9473-4739Fryad Z. Henari5https://orcid.org/0009-0008-8863-8645Department Medical Sciences, Royal College of Surgeons in Ireland, Medical University of Bahrain, P.O. Box 15503, Busaiteen 228, Kingdom of BahrainDepartment Medical Sciences, Royal College of Surgeons in Ireland, Medical University of Bahrain, P.O. Box 15503, Busaiteen 228, Kingdom of BahrainDepartment Medical Sciences, Royal College of Surgeons in Ireland, Medical University of Bahrain, P.O. Box 15503, Busaiteen 228, Kingdom of BahrainDepartment Medical Sciences, Royal College of Surgeons in Ireland, Medical University of Bahrain, P.O. Box 15503, Busaiteen 228, Kingdom of BahrainDepartment of Biophysics, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi ArabiaDepartment Medical Sciences, Royal College of Surgeons in Ireland, Medical University of Bahrain, P.O. Box 15503, Busaiteen 228, Kingdom of BahrainAim: To demonstrate a simple, eco-friendly, and cost-effective green method to synthesize gold nanoparticles (AuNPs) using the aqueous extract of gum Arabic (GA) as a reducing and stabilizing agent. Methods: Green synthesis of nanoparticles, characterization by absorption, infra-red and fluorescence spectroscopy. Results: The absorption spectrum (UV-Vis) showed an absorption peak ~522 nm corresponding to the surface plasmon resonance (SPR) absorption peak of AuNPs. Transmission electron microscopy (TEM) images revealed spherical-shaped nanoparticles with an average size of 15 nm. Fourier transform infrared (FTIR) analysis showed that the nanoparticles are coated with organic compounds that are present in GA. The fluorescence quenching properties of the AuNPs were assessed by monitoring their effects on fluorescence intensity of coumarin 153 (C153) dye. The fluorescence of the dye decreased with an increase in concentration of the nanoparticles. Upon addition of the protein bovine serum albumin (BSA) to the mixture the fluorescence increased (recovery) again. Conclusions: The fluorescence quenching and recovery (turn-on/off system) is a valuable method for protein detection in solution. By observing the effect of BSA on the quenched fluorescence, this nanoparticle system shows promise in biomedicine, drug delivery and environmental monitoring.https://www.explorationpub.com/uploads/Article/A101314/101314.pdfgreen synthesisgum arabicgold nanoparticlesfluorescence quenchingprotein detection |
| spellingShingle | Ahmed T. Algahiny Omar S. Elmitwalli Deyari A. Kassim G. Roshan Deen Sultan Akhtar Fryad Z. Henari Gum Arabic-assisted green synthesis of gold nanoparticles as fluorescence modulator for potential analytical applications Exploration of BioMat-X green synthesis gum arabic gold nanoparticles fluorescence quenching protein detection |
| title | Gum Arabic-assisted green synthesis of gold nanoparticles as fluorescence modulator for potential analytical applications |
| title_full | Gum Arabic-assisted green synthesis of gold nanoparticles as fluorescence modulator for potential analytical applications |
| title_fullStr | Gum Arabic-assisted green synthesis of gold nanoparticles as fluorescence modulator for potential analytical applications |
| title_full_unstemmed | Gum Arabic-assisted green synthesis of gold nanoparticles as fluorescence modulator for potential analytical applications |
| title_short | Gum Arabic-assisted green synthesis of gold nanoparticles as fluorescence modulator for potential analytical applications |
| title_sort | gum arabic assisted green synthesis of gold nanoparticles as fluorescence modulator for potential analytical applications |
| topic | green synthesis gum arabic gold nanoparticles fluorescence quenching protein detection |
| url | https://www.explorationpub.com/uploads/Article/A101314/101314.pdf |
| work_keys_str_mv | AT ahmedtalgahiny gumarabicassistedgreensynthesisofgoldnanoparticlesasfluorescencemodulatorforpotentialanalyticalapplications AT omarselmitwalli gumarabicassistedgreensynthesisofgoldnanoparticlesasfluorescencemodulatorforpotentialanalyticalapplications AT deyariakassim gumarabicassistedgreensynthesisofgoldnanoparticlesasfluorescencemodulatorforpotentialanalyticalapplications AT groshandeen gumarabicassistedgreensynthesisofgoldnanoparticlesasfluorescencemodulatorforpotentialanalyticalapplications AT sultanakhtar gumarabicassistedgreensynthesisofgoldnanoparticlesasfluorescencemodulatorforpotentialanalyticalapplications AT fryadzhenari gumarabicassistedgreensynthesisofgoldnanoparticlesasfluorescencemodulatorforpotentialanalyticalapplications |