Spoligotyping of tuberculous mycobacteria isolated from humans and cattle
Objective: to analyze the species and genetic families of tuberculous mycobacteria isolated from humans and cattle by spoligotyping.Subjects and methods. Biological materials collected in the cattle, livestock farmers (who underwent annual fluorographic examinations) and tuberculosis patients were s...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Russian |
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New Terra Publishing House
2020-04-01
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| Series: | Туберкулез и болезни лёгких |
| Subjects: | |
| Online Access: | https://www.tibl-journal.com/jour/article/view/1403 |
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| author | N. I. Khаmmаdov T. Kh. Faizov K. A. Osyanin A. V. Khаmmаdovа K. S. Khаertynov E. A. Shuralev |
| author_facet | N. I. Khаmmаdov T. Kh. Faizov K. A. Osyanin A. V. Khаmmаdovа K. S. Khаertynov E. A. Shuralev |
| author_sort | N. I. Khаmmаdov |
| collection | DOAJ |
| description | Objective: to analyze the species and genetic families of tuberculous mycobacteria isolated from humans and cattle by spoligotyping.Subjects and methods. Biological materials collected in the cattle, livestock farmers (who underwent annual fluorographic examinations) and tuberculosis patients were studied. To identify mycobacteria, the spoligotyping method was used.Results. The article assesses the variability of sites within direct repeats (spacers), which are used in laboratory diagnostics for spoligotyping of mycobacteria and identification of tuberculosis pathogens. The frequency of combinations of different spacers in the analyzed mycobacteria in the specimens identified as Mycobacterium tuberculosis complex is compared with the spoligotyping profile of known mycobacteria thus establishing their belonging to a specific genetic family The studied isolates were tested by microscopic, bacteriological, and molecular genetic (PCR) methods. Based on the results of spoligotyping, it was found out that they belonged to genetic families of M. tuberculosis of Beijing, LAM, and Haarlem. |
| format | Article |
| id | doaj-art-2956b96f685c4a99bcd516a680a47e84 |
| institution | DOAJ |
| issn | 2075-1230 2542-1506 |
| language | Russian |
| publishDate | 2020-04-01 |
| publisher | New Terra Publishing House |
| record_format | Article |
| series | Туберкулез и болезни лёгких |
| spelling | doaj-art-2956b96f685c4a99bcd516a680a47e842025-08-20T03:02:41ZrusNew Terra Publishing HouseТуберкулез и болезни лёгких2075-12302542-15062020-04-01983131810.21292/2075-1230-2020-98-3-13-181403Spoligotyping of tuberculous mycobacteria isolated from humans and cattleN. I. Khаmmаdov0T. Kh. Faizov1K. A. Osyanin2A. V. Khаmmаdovа3K. S. Khаertynov4E. A. Shuralev5Federal Center for Toxicological, Radiation and Biological SafetyFederal Center for Toxicological, Radiation and Biological SafetyFederal Center for Toxicological, Radiation and Biological SafetyKazan UniversityFederal Center for Toxicological, Radiation and Biological Safety; Kazan State Medical Academy, Branch of Russian Medical Academy for Professional DevelopmentFederal Center for Toxicological, Radiation and Biological Safety; Kazan University; Kazan State Medical Academy, Branch of Russian Medical Academy for Professional DevelopmentObjective: to analyze the species and genetic families of tuberculous mycobacteria isolated from humans and cattle by spoligotyping.Subjects and methods. Biological materials collected in the cattle, livestock farmers (who underwent annual fluorographic examinations) and tuberculosis patients were studied. To identify mycobacteria, the spoligotyping method was used.Results. The article assesses the variability of sites within direct repeats (spacers), which are used in laboratory diagnostics for spoligotyping of mycobacteria and identification of tuberculosis pathogens. The frequency of combinations of different spacers in the analyzed mycobacteria in the specimens identified as Mycobacterium tuberculosis complex is compared with the spoligotyping profile of known mycobacteria thus establishing their belonging to a specific genetic family The studied isolates were tested by microscopic, bacteriological, and molecular genetic (PCR) methods. Based on the results of spoligotyping, it was found out that they belonged to genetic families of M. tuberculosis of Beijing, LAM, and Haarlem.https://www.tibl-journal.com/jour/article/view/1403mycobacterium bovismycobacterium tuberculosistuberculosishumancattlespeciespcrspoligotyping |
| spellingShingle | N. I. Khаmmаdov T. Kh. Faizov K. A. Osyanin A. V. Khаmmаdovа K. S. Khаertynov E. A. Shuralev Spoligotyping of tuberculous mycobacteria isolated from humans and cattle Туберкулез и болезни лёгких mycobacterium bovis mycobacterium tuberculosis tuberculosis human cattle species pcr spoligotyping |
| title | Spoligotyping of tuberculous mycobacteria isolated from humans and cattle |
| title_full | Spoligotyping of tuberculous mycobacteria isolated from humans and cattle |
| title_fullStr | Spoligotyping of tuberculous mycobacteria isolated from humans and cattle |
| title_full_unstemmed | Spoligotyping of tuberculous mycobacteria isolated from humans and cattle |
| title_short | Spoligotyping of tuberculous mycobacteria isolated from humans and cattle |
| title_sort | spoligotyping of tuberculous mycobacteria isolated from humans and cattle |
| topic | mycobacterium bovis mycobacterium tuberculosis tuberculosis human cattle species pcr spoligotyping |
| url | https://www.tibl-journal.com/jour/article/view/1403 |
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