Protective effects of docosahexaenoic acid combined with bilberry extract on myopic Guinea pigs
This study aims to investigate the protective effects of docosahexaenoic acid (DHA) combined with bilberry extract (BE) on myopic guinea pigs. In total, 105 healthy pigmented guinea pigs aged 2 weeks were selected and randomly divided into five groups. The normal control (NC) group received no treat...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2024.1502612/full |
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| author | Tainan Lin Tainan Lin Jianzhang Hu Qian Wen Xiaoting Liu Jinghua Lin Qiaomei Shi Miao Lin Weifu Huang |
| author_facet | Tainan Lin Tainan Lin Jianzhang Hu Qian Wen Xiaoting Liu Jinghua Lin Qiaomei Shi Miao Lin Weifu Huang |
| author_sort | Tainan Lin |
| collection | DOAJ |
| description | This study aims to investigate the protective effects of docosahexaenoic acid (DHA) combined with bilberry extract (BE) on myopic guinea pigs. In total, 105 healthy pigmented guinea pigs aged 2 weeks were selected and randomly divided into five groups. The normal control (NC) group received no treatment, while the experimental groups wore −6.0D lenses on the right eye to establish an animal model of lens-induced myopia (LIM). These groups were further divided based on different treatments: normal feeding, DHA treatment, BE treatment, and combined DHA + BE treatment. Refractive error and axial length for both eyes were measured before modeling, after 4 weeks of modeling, and after 8 weeks of treatment. Fundus examination was performed, and choroidal thickness, choroidal vascularity index (CVI), maximal mixed response in dark adaptation (Max-ERG), and cone cell response in light adaptation (Cone-ERG) were measured. After 8 weeks of treatment, we observed a significant reduction in refractive error and shortening of axial length, improvement in fundus condition, and increased choroidal thickness and CVI in the LIM + DHA + BE group. Electroretinogram (ERG) showed that the amplitudes of a-wave and b-wave were enhanced in both Max-ERG and Cone-ERG tests. The LIM + DHA + BE group exhibited superior effects compared to the LIM + DHA group and the LIM + BE group. The combination of DHA and BE delayed the progression of LIM in guinea pigs and was more effective than DHA or BE alone. The synergistic effect of DHA and BE offers a new approach to the prevention and treatment of myopia. |
| format | Article |
| id | doaj-art-2947c16e95d048f391c8fff022962549 |
| institution | DOAJ |
| issn | 2296-858X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj-art-2947c16e95d048f391c8fff0229625492025-08-20T02:49:36ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2024-12-011110.3389/fmed.2024.15026121502612Protective effects of docosahexaenoic acid combined with bilberry extract on myopic Guinea pigsTainan Lin0Tainan Lin1Jianzhang Hu2Qian Wen3Xiaoting Liu4Jinghua Lin5Qiaomei Shi6Miao Lin7Weifu Huang8Department of Ophthalmology, Fujian Medical University Union Hospital, Fuzhou, ChinaDepartment of Ophthalmology, Fujian Provincial Governmental Hospital, Fuzhou, ChinaDepartment of Ophthalmology, Fujian Medical University Union Hospital, Fuzhou, ChinaDepartment of Ophthalmology, Fujian Provincial Governmental Hospital, Fuzhou, ChinaDepartment of Ophthalmology, Fujian Provincial Governmental Hospital, Fuzhou, ChinaDepartment of Ophthalmology, Fujian Provincial Governmental Hospital, Fuzhou, ChinaDepartment of Ophthalmology, Fujian Provincial Governmental Hospital, Fuzhou, ChinaDepartment of Ophthalmology, Fujian Provincial Governmental Hospital, Fuzhou, ChinaTowardPi Medical Technology Beijing, Beijing, ChinaThis study aims to investigate the protective effects of docosahexaenoic acid (DHA) combined with bilberry extract (BE) on myopic guinea pigs. In total, 105 healthy pigmented guinea pigs aged 2 weeks were selected and randomly divided into five groups. The normal control (NC) group received no treatment, while the experimental groups wore −6.0D lenses on the right eye to establish an animal model of lens-induced myopia (LIM). These groups were further divided based on different treatments: normal feeding, DHA treatment, BE treatment, and combined DHA + BE treatment. Refractive error and axial length for both eyes were measured before modeling, after 4 weeks of modeling, and after 8 weeks of treatment. Fundus examination was performed, and choroidal thickness, choroidal vascularity index (CVI), maximal mixed response in dark adaptation (Max-ERG), and cone cell response in light adaptation (Cone-ERG) were measured. After 8 weeks of treatment, we observed a significant reduction in refractive error and shortening of axial length, improvement in fundus condition, and increased choroidal thickness and CVI in the LIM + DHA + BE group. Electroretinogram (ERG) showed that the amplitudes of a-wave and b-wave were enhanced in both Max-ERG and Cone-ERG tests. The LIM + DHA + BE group exhibited superior effects compared to the LIM + DHA group and the LIM + BE group. The combination of DHA and BE delayed the progression of LIM in guinea pigs and was more effective than DHA or BE alone. The synergistic effect of DHA and BE offers a new approach to the prevention and treatment of myopia.https://www.frontiersin.org/articles/10.3389/fmed.2024.1502612/fulldocosahexaenoic acidbilberry extractlens-induced myopiachoroidal thicknesschoroidal vascularity indexGuinea pigs |
| spellingShingle | Tainan Lin Tainan Lin Jianzhang Hu Qian Wen Xiaoting Liu Jinghua Lin Qiaomei Shi Miao Lin Weifu Huang Protective effects of docosahexaenoic acid combined with bilberry extract on myopic Guinea pigs Frontiers in Medicine docosahexaenoic acid bilberry extract lens-induced myopia choroidal thickness choroidal vascularity index Guinea pigs |
| title | Protective effects of docosahexaenoic acid combined with bilberry extract on myopic Guinea pigs |
| title_full | Protective effects of docosahexaenoic acid combined with bilberry extract on myopic Guinea pigs |
| title_fullStr | Protective effects of docosahexaenoic acid combined with bilberry extract on myopic Guinea pigs |
| title_full_unstemmed | Protective effects of docosahexaenoic acid combined with bilberry extract on myopic Guinea pigs |
| title_short | Protective effects of docosahexaenoic acid combined with bilberry extract on myopic Guinea pigs |
| title_sort | protective effects of docosahexaenoic acid combined with bilberry extract on myopic guinea pigs |
| topic | docosahexaenoic acid bilberry extract lens-induced myopia choroidal thickness choroidal vascularity index Guinea pigs |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2024.1502612/full |
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