Group II phospholipase A2 in human gingiva with periodontal disease

Fourteen kilodalton phospholipase A2 molecules (PLA2) are classified into two groups, I- and II-PLA2, and only the latter has been considered to play a pathogenetic role in various forms of tissue inflammation. Previously we demonstrated high PLA2 activity in gingival crevicular fluid (GCF) of patie...

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Main Authors: H. Shinohara, T. Komatsubara, H. Tojo, M. Okamoto, S. Nishikawa, T. Nagata, H. Ishida
Format: Article
Language:English
Published: Wiley 1995-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/S0962935195000159
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author H. Shinohara
T. Komatsubara
H. Tojo
M. Okamoto
S. Nishikawa
T. Nagata
H. Ishida
author_facet H. Shinohara
T. Komatsubara
H. Tojo
M. Okamoto
S. Nishikawa
T. Nagata
H. Ishida
author_sort H. Shinohara
collection DOAJ
description Fourteen kilodalton phospholipase A2 molecules (PLA2) are classified into two groups, I- and II-PLA2, and only the latter has been considered to play a pathogenetic role in various forms of tissue inflammation. Previously we demonstrated high PLA2 activity in gingival crevicular fluid (GCF) of patients with periodontal disease, without determining the group of the enzyme involved. In this study, the activity, groups and levels of enzyme in gingiva taken from 13 sites of periodontal disease were determined using both biochemical and radioimmunological methods. A linear correlation between the activity and the level of II-PLA2 was observed. No I-PLA2 was found in any of the samples tested. These data suggest that the PLA2 activity found in the GCF of patients with periodontal disease does not belong to the I-PLA2 but to the II-PLA2 group.
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issn 0962-9351
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publishDate 1995-01-01
publisher Wiley
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series Mediators of Inflammation
spelling doaj-art-292fca6785e44625aa5b968ff86dfe352025-08-20T02:03:54ZengWileyMediators of Inflammation0962-93511466-18611995-01-0142959710.1155/S0962935195000159Group II phospholipase A2 in human gingiva with periodontal diseaseH. Shinohara0T. Komatsubara1H. Tojo2M. Okamoto3S. Nishikawa4T. Nagata5H. Ishida6Department of Periodontology and Endodontology, School of Dentistry, the University of Tokushima, 3 Kuramoto-cho, Tokushima 770, JapanDepartment of Molecular Physiological Chemistry, Osaka University Medical School, 2 Yamadaoka, Suita, Osaka 565, JapanDepartment of Molecular Physiological Chemistry, Osaka University Medical School, 2 Yamadaoka, Suita, Osaka 565, JapanDepartment of Molecular Physiological Chemistry, Osaka University Medical School, 2 Yamadaoka, Suita, Osaka 565, JapanDepartment of Periodontology and Endodontology, School of Dentistry, the University of Tokushima, 3 Kuramoto-cho, Tokushima 770, JapanDepartment of Periodontology and Endodontology, School of Dentistry, the University of Tokushima, 3 Kuramoto-cho, Tokushima 770, JapanDepartment of Periodontology and Endodontology, School of Dentistry, the University of Tokushima, 3 Kuramoto-cho, Tokushima 770, JapanFourteen kilodalton phospholipase A2 molecules (PLA2) are classified into two groups, I- and II-PLA2, and only the latter has been considered to play a pathogenetic role in various forms of tissue inflammation. Previously we demonstrated high PLA2 activity in gingival crevicular fluid (GCF) of patients with periodontal disease, without determining the group of the enzyme involved. In this study, the activity, groups and levels of enzyme in gingiva taken from 13 sites of periodontal disease were determined using both biochemical and radioimmunological methods. A linear correlation between the activity and the level of II-PLA2 was observed. No I-PLA2 was found in any of the samples tested. These data suggest that the PLA2 activity found in the GCF of patients with periodontal disease does not belong to the I-PLA2 but to the II-PLA2 group.http://dx.doi.org/10.1155/S0962935195000159
spellingShingle H. Shinohara
T. Komatsubara
H. Tojo
M. Okamoto
S. Nishikawa
T. Nagata
H. Ishida
Group II phospholipase A2 in human gingiva with periodontal disease
Mediators of Inflammation
title Group II phospholipase A2 in human gingiva with periodontal disease
title_full Group II phospholipase A2 in human gingiva with periodontal disease
title_fullStr Group II phospholipase A2 in human gingiva with periodontal disease
title_full_unstemmed Group II phospholipase A2 in human gingiva with periodontal disease
title_short Group II phospholipase A2 in human gingiva with periodontal disease
title_sort group ii phospholipase a2 in human gingiva with periodontal disease
url http://dx.doi.org/10.1155/S0962935195000159
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