Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits
Myostatin (MSTN) protein, lncRNAs, and circRNAs regulate skeletal muscle growth and development. This work aims to compare the expression patterns of circRNAs and lncRNAs in the gluteus maximus tissue of wild-type (WT) and MSTN gene knockout (KO) rabbits. Within the gluteus maximus tissue of three W...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Animal Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10495398.2025.2465624 |
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| author | Ling Li Ting Zhang Muhammad Farhab Xiao-Xiao Xia Abu Musa Md Talimur Reza Paing Oo Kyaw Fenglei Chen Esraa Aly Sayed Ismail Gang Xue Ping Zhong Yong Cheng Yu-Guo Yuan |
| author_facet | Ling Li Ting Zhang Muhammad Farhab Xiao-Xiao Xia Abu Musa Md Talimur Reza Paing Oo Kyaw Fenglei Chen Esraa Aly Sayed Ismail Gang Xue Ping Zhong Yong Cheng Yu-Guo Yuan |
| author_sort | Ling Li |
| collection | DOAJ |
| description | Myostatin (MSTN) protein, lncRNAs, and circRNAs regulate skeletal muscle growth and development. This work aims to compare the expression patterns of circRNAs and lncRNAs in the gluteus maximus tissue of wild-type (WT) and MSTN gene knockout (KO) rabbits. Within the gluteus maximus tissue of three WT and four MSTN KO rabbits, we analyzed the expression profiles of circRNAs and lncRNAs. After identifying the differently expressed RNAs, the biological pathways implicated were ascertained by performing enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). We identified differences in the expression of 251 circRNAs (79 upregulated and 172 downregulated), 176 lncRNAs (53 upregulated and 123 downregulated), and 1178 mRNAs (408 upregulated and 770 downregulated) between WT and MSTN KO rabbits. Target genes were significantly enriched in pathways associated with protein synthesis and catabolism, such as oxidative phosphorylation, ubiquitin-mediated proteolysis, the FoxO signaling pathway, and the pentose phosphate pathway, as identified through GO and KEGG enrichment analyses. The constructed network indicates that a class of circRNAs and lncRNAs is engaged in MSTN-mediated regulation of skeletal muscle development. These findings provide valuable insights for innovative therapeutic, diagnostic, and preventive approaches to muscle disorders. |
| format | Article |
| id | doaj-art-2928c9f39cfc40caabec69d02b3bf44b |
| institution | OA Journals |
| issn | 1049-5398 1532-2378 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Biotechnology |
| spelling | doaj-art-2928c9f39cfc40caabec69d02b3bf44b2025-08-20T02:30:05ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782025-12-0136110.1080/10495398.2025.2465624Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbitsLing Li0Ting Zhang1Muhammad Farhab2Xiao-Xiao Xia3Abu Musa Md Talimur Reza4Paing Oo Kyaw5Fenglei Chen6Esraa Aly Sayed Ismail7Gang Xue8Ping Zhong9Yong Cheng10Yu-Guo Yuan11College of Veterinary Medicine/Key Laboratory of Animal Genetic Engineering, Yangzhou University, Jiangsu, Yangzhou, ChinaSchool of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and Forestry, Jurong, ChinaCollege of Veterinary Medicine/Key Laboratory of Animal Genetic Engineering, Yangzhou University, Jiangsu, Yangzhou, ChinaCollege of Veterinary Medicine/Key Laboratory of Animal Genetic Engineering, Yangzhou University, Jiangsu, Yangzhou, ChinaDepartment of Molecular Biology and Genetics, Faculty of Basic Sciences, Gebze Technical University, Gebze, Kocaeli, Republic of TurkiyeCollege of Veterinary Medicine/Key Laboratory of Animal Genetic Engineering, Yangzhou University, Jiangsu, Yangzhou, ChinaCollege of Veterinary Medicine/Key Laboratory of Animal Genetic Engineering, Yangzhou University, Jiangsu, Yangzhou, ChinaVeterinary Research Division, National Research Center, EgyptNantong City Haimen District Yangtze River Delta White Goat Breeding Research Institute, Jiangsu, Nantong, ChinaCollege of Veterinary Medicine/Key Laboratory of Animal Genetic Engineering, Yangzhou University, Jiangsu, Yangzhou, ChinaCollege of Veterinary Medicine/Key Laboratory of Animal Genetic Engineering, Yangzhou University, Jiangsu, Yangzhou, ChinaCollege of Veterinary Medicine/Key Laboratory of Animal Genetic Engineering, Yangzhou University, Jiangsu, Yangzhou, ChinaMyostatin (MSTN) protein, lncRNAs, and circRNAs regulate skeletal muscle growth and development. This work aims to compare the expression patterns of circRNAs and lncRNAs in the gluteus maximus tissue of wild-type (WT) and MSTN gene knockout (KO) rabbits. Within the gluteus maximus tissue of three WT and four MSTN KO rabbits, we analyzed the expression profiles of circRNAs and lncRNAs. After identifying the differently expressed RNAs, the biological pathways implicated were ascertained by performing enrichment analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). We identified differences in the expression of 251 circRNAs (79 upregulated and 172 downregulated), 176 lncRNAs (53 upregulated and 123 downregulated), and 1178 mRNAs (408 upregulated and 770 downregulated) between WT and MSTN KO rabbits. Target genes were significantly enriched in pathways associated with protein synthesis and catabolism, such as oxidative phosphorylation, ubiquitin-mediated proteolysis, the FoxO signaling pathway, and the pentose phosphate pathway, as identified through GO and KEGG enrichment analyses. The constructed network indicates that a class of circRNAs and lncRNAs is engaged in MSTN-mediated regulation of skeletal muscle development. These findings provide valuable insights for innovative therapeutic, diagnostic, and preventive approaches to muscle disorders.https://www.tandfonline.com/doi/10.1080/10495398.2025.2465624MyostatincircRNAlncRNAskeletal muscle growthgene knockout |
| spellingShingle | Ling Li Ting Zhang Muhammad Farhab Xiao-Xiao Xia Abu Musa Md Talimur Reza Paing Oo Kyaw Fenglei Chen Esraa Aly Sayed Ismail Gang Xue Ping Zhong Yong Cheng Yu-Guo Yuan Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits Animal Biotechnology Myostatin circRNA lncRNA skeletal muscle growth gene knockout |
| title | Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits |
| title_full | Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits |
| title_fullStr | Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits |
| title_full_unstemmed | Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits |
| title_short | Comprehensive analysis of circRNAs and lncRNAs involvement in the development of skeletal muscle in myostatin-deficient rabbits |
| title_sort | comprehensive analysis of circrnas and lncrnas involvement in the development of skeletal muscle in myostatin deficient rabbits |
| topic | Myostatin circRNA lncRNA skeletal muscle growth gene knockout |
| url | https://www.tandfonline.com/doi/10.1080/10495398.2025.2465624 |
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