Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100

We show that galactomannan, a polysaccharide consisting of a mannose backbone with galactose side groups present on the cell wall of several fungi, induces a reprogramming of the inflammatory response in human macrophages through dectin-1 receptor. The nuclear factor kappa-light-chain-enhancer of ac...

Full description

Saved in:
Bibliographic Details
Main Authors: Víctor Toledano, Enrique Hernández-Jiménez, Carolina Cubillos-Zapata, Marta Flandez, Enrique Álvarez, Aníbal Varela-Serrano, Ramón Cantero, Gema Valles, Francisco García-Rio, Eduardo López-Collazo
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2015/942517
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850230412633178112
author Víctor Toledano
Enrique Hernández-Jiménez
Carolina Cubillos-Zapata
Marta Flandez
Enrique Álvarez
Aníbal Varela-Serrano
Ramón Cantero
Gema Valles
Francisco García-Rio
Eduardo López-Collazo
author_facet Víctor Toledano
Enrique Hernández-Jiménez
Carolina Cubillos-Zapata
Marta Flandez
Enrique Álvarez
Aníbal Varela-Serrano
Ramón Cantero
Gema Valles
Francisco García-Rio
Eduardo López-Collazo
author_sort Víctor Toledano
collection DOAJ
description We show that galactomannan, a polysaccharide consisting of a mannose backbone with galactose side groups present on the cell wall of several fungi, induces a reprogramming of the inflammatory response in human macrophages through dectin-1 receptor. The nuclear factor kappa-light-chain-enhancer of activated B cells 2 (NFκB2)/p100 was overexpressed after galactomannan challenge. Knocking down NFκB2/p100 using small interfering RNA (siRNA) indicated that NFκB2/p100 expression is a crucial factor in the progression of the galactomannan-induced refractoriness. The data presented in this study could be used as a modulator of inflammatory response in clinical situations where refractory state is required.
format Article
id doaj-art-290e519d42e7487bbbe9248132565fa1
institution OA Journals
issn 0962-9351
1466-1861
language English
publishDate 2015-01-01
publisher Wiley
record_format Article
series Mediators of Inflammation
spelling doaj-art-290e519d42e7487bbbe9248132565fa12025-08-20T02:03:52ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/942517942517Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100Víctor Toledano0Enrique Hernández-Jiménez1Carolina Cubillos-Zapata2Marta Flandez3Enrique Álvarez4Aníbal Varela-Serrano5Ramón Cantero6Gema Valles7Francisco García-Rio8Eduardo López-Collazo9Tumor Immunology Laboratory, IdiPAZ, La Paz Hospital, 28046 Madrid, SpainTumor Immunology Laboratory, IdiPAZ, La Paz Hospital, 28046 Madrid, SpainTumor Immunology Laboratory, IdiPAZ, La Paz Hospital, 28046 Madrid, SpainTumor Immunology Laboratory, IdiPAZ, La Paz Hospital, 28046 Madrid, SpainInnate Immunity Group, IdiPAZ, La Paz Hospital, 28046 Madrid, SpainTumor Immunology Laboratory, IdiPAZ, La Paz Hospital, 28046 Madrid, SpainInnate Immunity Group, IdiPAZ, La Paz Hospital, 28046 Madrid, SpainCIBER-BBN, 28029 Madrid, SpainRespiratory Disease, IdiPAZ, La Paz Hospital, 28046 Madrid, SpainTumor Immunology Laboratory, IdiPAZ, La Paz Hospital, 28046 Madrid, SpainWe show that galactomannan, a polysaccharide consisting of a mannose backbone with galactose side groups present on the cell wall of several fungi, induces a reprogramming of the inflammatory response in human macrophages through dectin-1 receptor. The nuclear factor kappa-light-chain-enhancer of activated B cells 2 (NFκB2)/p100 was overexpressed after galactomannan challenge. Knocking down NFκB2/p100 using small interfering RNA (siRNA) indicated that NFκB2/p100 expression is a crucial factor in the progression of the galactomannan-induced refractoriness. The data presented in this study could be used as a modulator of inflammatory response in clinical situations where refractory state is required.http://dx.doi.org/10.1155/2015/942517
spellingShingle Víctor Toledano
Enrique Hernández-Jiménez
Carolina Cubillos-Zapata
Marta Flandez
Enrique Álvarez
Aníbal Varela-Serrano
Ramón Cantero
Gema Valles
Francisco García-Rio
Eduardo López-Collazo
Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100
Mediators of Inflammation
title Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100
title_full Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100
title_fullStr Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100
title_full_unstemmed Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100
title_short Galactomannan Downregulates the Inflammation Responses in Human Macrophages via NFκB2/p100
title_sort galactomannan downregulates the inflammation responses in human macrophages via nfκb2 p100
url http://dx.doi.org/10.1155/2015/942517
work_keys_str_mv AT victortoledano galactomannandownregulatestheinflammationresponsesinhumanmacrophagesvianfkb2p100
AT enriquehernandezjimenez galactomannandownregulatestheinflammationresponsesinhumanmacrophagesvianfkb2p100
AT carolinacubilloszapata galactomannandownregulatestheinflammationresponsesinhumanmacrophagesvianfkb2p100
AT martaflandez galactomannandownregulatestheinflammationresponsesinhumanmacrophagesvianfkb2p100
AT enriquealvarez galactomannandownregulatestheinflammationresponsesinhumanmacrophagesvianfkb2p100
AT anibalvarelaserrano galactomannandownregulatestheinflammationresponsesinhumanmacrophagesvianfkb2p100
AT ramoncantero galactomannandownregulatestheinflammationresponsesinhumanmacrophagesvianfkb2p100
AT gemavalles galactomannandownregulatestheinflammationresponsesinhumanmacrophagesvianfkb2p100
AT franciscogarciario galactomannandownregulatestheinflammationresponsesinhumanmacrophagesvianfkb2p100
AT eduardolopezcollazo galactomannandownregulatestheinflammationresponsesinhumanmacrophagesvianfkb2p100