Metabolomic analysis of rat arterial serum under hypobaric hypoxia: Adaptive regulation of physiological systems by metabolic reprogramming
Objective: To investigate the associations between metabolic changes and functions, including energy metabolism, immune response, and redox balance, under short-term hypobaric hypoxia exposure. Non-targeted metabolomics and bioinformatics analysis were applied to explore the adaptive mechanisms of o...
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Elsevier
2025-03-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580825000305 |
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| author | Dengqin Ma Bing Li Bang Xin Bingfang Xie Enpen Zhu Zihao Zhang Xiaoqin Ha |
| author_facet | Dengqin Ma Bing Li Bang Xin Bingfang Xie Enpen Zhu Zihao Zhang Xiaoqin Ha |
| author_sort | Dengqin Ma |
| collection | DOAJ |
| description | Objective: To investigate the associations between metabolic changes and functions, including energy metabolism, immune response, and redox balance, under short-term hypobaric hypoxia exposure. Non-targeted metabolomics and bioinformatics analysis were applied to explore the adaptive mechanisms of organisms in hypobaric hypoxia. Methods: Healthy adult male Sprague—Dawley rats were placed in environments simulating altitudes of 6500 m (HC group) and 1588 m (Control group). After 14 days, arterial serum samples were analyzed using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). Significant metabolites (P < 0.05, VIP >1) were identified, and KEGG enrichment analysis was conducted. Differential metabolites were globally analyzed with MetaboAnalyst 5.0. Results: A total of 117 significantly altered metabolites were identified. In the HC group, 84 metabolites significantly increased, while 33 metabolites significantly decreased compared to the Control group. KEGG enrichment analysis revealed significant metabolic pathways, including the PPAR signaling pathway, bile secretion, arginine biosynthesis, alcoholism, and cholesterol metabolism (P < 0.05). Global analysis indicated that these differential metabolites were involved in various pathways, such as energy metabolism, amino acid metabolism, nucleotide metabolism, lipid metabolism, vitamin and cofactor metabolism, steroid metabolism, neurotransmitter metabolism, and heme metabolism, all of which play crucial roles in multiple biological processes. Conclusion: Short-term hypobaric hypoxia exposure significantly altered the metabolite profiles in the arterial serum samples of rats, revealing adaptive metabolic reprogramming in energy metabolism, redox balance, immune function, endocrine regulation, and neurological systems. |
| format | Article |
| id | doaj-art-2908e1d98913408b92984ec6ccc4f3ea |
| institution | DOAJ |
| issn | 2405-5808 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Biochemistry and Biophysics Reports |
| spelling | doaj-art-2908e1d98913408b92984ec6ccc4f3ea2025-08-20T03:15:16ZengElsevierBiochemistry and Biophysics Reports2405-58082025-03-014110194310.1016/j.bbrep.2025.101943Metabolomic analysis of rat arterial serum under hypobaric hypoxia: Adaptive regulation of physiological systems by metabolic reprogrammingDengqin Ma0Bing Li1Bang Xin2Bingfang Xie3Enpen Zhu4Zihao Zhang5Xiaoqin Ha6School of Basic Medical Sciences, Gansu University of Chinese Medicine, Lanzhou, 730099, China; The 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou, 730099, China; The First People's Hospital of Tianshui, Tianshui, 741000, Gansu, ChinaThe 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou, 730099, ChinaSchool of Basic Medical Sciences, Gansu University of Chinese Medicine, Lanzhou, 730099, China; The 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou, 730099, ChinaThe First People's Hospital of Tianshui, Tianshui, 741000, Gansu, ChinaSchool of Basic Medical Sciences, Gansu University of Chinese Medicine, Lanzhou, 730099, China; The 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou, 730099, ChinaThe 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou, 730099, ChinaThe 940th Hospital of Joint Logistics Support Force of Chinese People's Liberation Army, Lanzhou, 730099, China; Corresponding author.Objective: To investigate the associations between metabolic changes and functions, including energy metabolism, immune response, and redox balance, under short-term hypobaric hypoxia exposure. Non-targeted metabolomics and bioinformatics analysis were applied to explore the adaptive mechanisms of organisms in hypobaric hypoxia. Methods: Healthy adult male Sprague—Dawley rats were placed in environments simulating altitudes of 6500 m (HC group) and 1588 m (Control group). After 14 days, arterial serum samples were analyzed using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). Significant metabolites (P < 0.05, VIP >1) were identified, and KEGG enrichment analysis was conducted. Differential metabolites were globally analyzed with MetaboAnalyst 5.0. Results: A total of 117 significantly altered metabolites were identified. In the HC group, 84 metabolites significantly increased, while 33 metabolites significantly decreased compared to the Control group. KEGG enrichment analysis revealed significant metabolic pathways, including the PPAR signaling pathway, bile secretion, arginine biosynthesis, alcoholism, and cholesterol metabolism (P < 0.05). Global analysis indicated that these differential metabolites were involved in various pathways, such as energy metabolism, amino acid metabolism, nucleotide metabolism, lipid metabolism, vitamin and cofactor metabolism, steroid metabolism, neurotransmitter metabolism, and heme metabolism, all of which play crucial roles in multiple biological processes. Conclusion: Short-term hypobaric hypoxia exposure significantly altered the metabolite profiles in the arterial serum samples of rats, revealing adaptive metabolic reprogramming in energy metabolism, redox balance, immune function, endocrine regulation, and neurological systems.http://www.sciencedirect.com/science/article/pii/S2405580825000305Hypobaric hypoxiaMetabolomicsMetabolic reprogrammingEnergy metabolismRedox homeostasisImmune regulation |
| spellingShingle | Dengqin Ma Bing Li Bang Xin Bingfang Xie Enpen Zhu Zihao Zhang Xiaoqin Ha Metabolomic analysis of rat arterial serum under hypobaric hypoxia: Adaptive regulation of physiological systems by metabolic reprogramming Biochemistry and Biophysics Reports Hypobaric hypoxia Metabolomics Metabolic reprogramming Energy metabolism Redox homeostasis Immune regulation |
| title | Metabolomic analysis of rat arterial serum under hypobaric hypoxia: Adaptive regulation of physiological systems by metabolic reprogramming |
| title_full | Metabolomic analysis of rat arterial serum under hypobaric hypoxia: Adaptive regulation of physiological systems by metabolic reprogramming |
| title_fullStr | Metabolomic analysis of rat arterial serum under hypobaric hypoxia: Adaptive regulation of physiological systems by metabolic reprogramming |
| title_full_unstemmed | Metabolomic analysis of rat arterial serum under hypobaric hypoxia: Adaptive regulation of physiological systems by metabolic reprogramming |
| title_short | Metabolomic analysis of rat arterial serum under hypobaric hypoxia: Adaptive regulation of physiological systems by metabolic reprogramming |
| title_sort | metabolomic analysis of rat arterial serum under hypobaric hypoxia adaptive regulation of physiological systems by metabolic reprogramming |
| topic | Hypobaric hypoxia Metabolomics Metabolic reprogramming Energy metabolism Redox homeostasis Immune regulation |
| url | http://www.sciencedirect.com/science/article/pii/S2405580825000305 |
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