Effect of storage conditions and duration of erythrocyte mass and whole blood of dogs on mean erythrocyte volume and erythrocyte hemolysis
The study investigates the impact of storage conditions and duration on the mean corpuscular volume (MCV) and hemolysis levels of erythrocytes in canine whole blood and canine packed red blood cells (pRBC). The objects of the research were whole blood and pRBC obtained from healthy donor dogs and st...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv
2024-12-01
|
| Series: | Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки |
| Subjects: | |
| Online Access: | https://nvlvet.com.ua/index.php/journal/article/view/5411 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849706292079230976 |
|---|---|
| author | I. M. Yakymchuk |
| author_facet | I. M. Yakymchuk |
| author_sort | I. M. Yakymchuk |
| collection | DOAJ |
| description | The study investigates the impact of storage conditions and duration on the mean corpuscular volume (MCV) and hemolysis levels of erythrocytes in canine whole blood and canine packed red blood cells (pRBC). The objects of the research were whole blood and pRBC obtained from healthy donor dogs and stored in a blood bank for 28 days. The results demonstrated significant morphological changes in erythrocytes during storage. Specifically, the MCV gradually increased, indicating membrane damage, dysfunction of the Na-K pump, and intracellular fluid accumulation. The increase in MCV was more pronounced in erythrocytes from whole blood compared to pRBC. This growth in MCV may reflect changes in erythrocyte shape, leading to impaired rheological properties and an elevated risk of microcirculatory thrombosis in recipients. The hemolysis level of erythrocytes also rose with longer storage durations. In whole blood, this parameter exceeded 1 % as early as the 14th day of storage, whereas in canine pRBC such a level was observed only on the 28th day. According to human blood standards, this level of hemolysis is critical and necessitates product disposal. The study also found that separating whole blood into components reduces the intensity of hemolysis, which could optimize blood storage practices in veterinary transfusion medicine. The research emphasizes the need to establish clear standards for storing canine blood products, taking into account storage duration and morphological changes in erythrocytes. Specifically, it recommends shortening the storage time for whole blood and increasing the proportion of blood separation during donation. The findings aim to reduce complications during transfusions in veterinary practice, thereby improving their efficacy and safety. |
| format | Article |
| id | doaj-art-290211cd45214a3b9bd2dbe5fb81fcdf |
| institution | DOAJ |
| issn | 2518-7554 2518-1327 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv |
| record_format | Article |
| series | Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки |
| spelling | doaj-art-290211cd45214a3b9bd2dbe5fb81fcdf2025-08-20T03:16:14ZengStepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies LvivНауковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки2518-75542518-13272024-12-012611616717310.32718/nvlvet116255411Effect of storage conditions and duration of erythrocyte mass and whole blood of dogs on mean erythrocyte volume and erythrocyte hemolysisI. M. Yakymchuk0National University of Life and Environmental Sciences of Ukraine, Kyiv, UkraineThe study investigates the impact of storage conditions and duration on the mean corpuscular volume (MCV) and hemolysis levels of erythrocytes in canine whole blood and canine packed red blood cells (pRBC). The objects of the research were whole blood and pRBC obtained from healthy donor dogs and stored in a blood bank for 28 days. The results demonstrated significant morphological changes in erythrocytes during storage. Specifically, the MCV gradually increased, indicating membrane damage, dysfunction of the Na-K pump, and intracellular fluid accumulation. The increase in MCV was more pronounced in erythrocytes from whole blood compared to pRBC. This growth in MCV may reflect changes in erythrocyte shape, leading to impaired rheological properties and an elevated risk of microcirculatory thrombosis in recipients. The hemolysis level of erythrocytes also rose with longer storage durations. In whole blood, this parameter exceeded 1 % as early as the 14th day of storage, whereas in canine pRBC such a level was observed only on the 28th day. According to human blood standards, this level of hemolysis is critical and necessitates product disposal. The study also found that separating whole blood into components reduces the intensity of hemolysis, which could optimize blood storage practices in veterinary transfusion medicine. The research emphasizes the need to establish clear standards for storing canine blood products, taking into account storage duration and morphological changes in erythrocytes. Specifically, it recommends shortening the storage time for whole blood and increasing the proportion of blood separation during donation. The findings aim to reduce complications during transfusions in veterinary practice, thereby improving their efficacy and safety.https://nvlvet.com.ua/index.php/journal/article/view/5411packed red blood cells, whole blood, dogs, hemolysis, mean corpuscular volume, storage, donors, transfu-sion. |
| spellingShingle | I. M. Yakymchuk Effect of storage conditions and duration of erythrocyte mass and whole blood of dogs on mean erythrocyte volume and erythrocyte hemolysis Науковий вісник Львівського національного університету ветеринарної медицини та біотехнологій імені С.З. Ґжицького: Серія Ветеринарні науки packed red blood cells, whole blood, dogs, hemolysis, mean corpuscular volume, storage, donors, transfu-sion. |
| title | Effect of storage conditions and duration of erythrocyte mass and whole blood of dogs on mean erythrocyte volume and erythrocyte hemolysis |
| title_full | Effect of storage conditions and duration of erythrocyte mass and whole blood of dogs on mean erythrocyte volume and erythrocyte hemolysis |
| title_fullStr | Effect of storage conditions and duration of erythrocyte mass and whole blood of dogs on mean erythrocyte volume and erythrocyte hemolysis |
| title_full_unstemmed | Effect of storage conditions and duration of erythrocyte mass and whole blood of dogs on mean erythrocyte volume and erythrocyte hemolysis |
| title_short | Effect of storage conditions and duration of erythrocyte mass and whole blood of dogs on mean erythrocyte volume and erythrocyte hemolysis |
| title_sort | effect of storage conditions and duration of erythrocyte mass and whole blood of dogs on mean erythrocyte volume and erythrocyte hemolysis |
| topic | packed red blood cells, whole blood, dogs, hemolysis, mean corpuscular volume, storage, donors, transfu-sion. |
| url | https://nvlvet.com.ua/index.php/journal/article/view/5411 |
| work_keys_str_mv | AT imyakymchuk effectofstorageconditionsanddurationoferythrocytemassandwholebloodofdogsonmeanerythrocytevolumeanderythrocytehemolysis |