A framework for conducting GWAS using repeated measures data with an application to childhood BMI

Abstract Genetic effects on changes in human traits over time are understudied and may have important pathophysiological impact. We propose a framework that enables data quality control, implements mixed models to evaluate trajectories of change in traits, and estimates phenotypes to identify age-va...

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Main Authors: Kimberley Burrows, Anni Heiskala, Jonathan P. Bradfield, Zhanna Balkhiyarova, Lijiao Ning, Mathilde Boissel, Yee-Ming Chan, Philippe Froguel, Amelie Bonnefond, Hakon Hakonarson, Alexessander Couto Alves, Deborah A. Lawlor, Marika Kaakinen, Marjo-Riitta Järvelin, Struan F. A. Grant, Kate Tilling, Inga Prokopenko, Sylvain Sebert, Mickaël Canouil, Nicole M. Warrington
Format: Article
Language:English
Published: Nature Portfolio 2024-11-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-024-53687-3
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author Kimberley Burrows
Anni Heiskala
Jonathan P. Bradfield
Zhanna Balkhiyarova
Lijiao Ning
Mathilde Boissel
Yee-Ming Chan
Philippe Froguel
Amelie Bonnefond
Hakon Hakonarson
Alexessander Couto Alves
Deborah A. Lawlor
Marika Kaakinen
Marjo-Riitta Järvelin
Struan F. A. Grant
Kate Tilling
Inga Prokopenko
Sylvain Sebert
Mickaël Canouil
Nicole M. Warrington
author_facet Kimberley Burrows
Anni Heiskala
Jonathan P. Bradfield
Zhanna Balkhiyarova
Lijiao Ning
Mathilde Boissel
Yee-Ming Chan
Philippe Froguel
Amelie Bonnefond
Hakon Hakonarson
Alexessander Couto Alves
Deborah A. Lawlor
Marika Kaakinen
Marjo-Riitta Järvelin
Struan F. A. Grant
Kate Tilling
Inga Prokopenko
Sylvain Sebert
Mickaël Canouil
Nicole M. Warrington
author_sort Kimberley Burrows
collection DOAJ
description Abstract Genetic effects on changes in human traits over time are understudied and may have important pathophysiological impact. We propose a framework that enables data quality control, implements mixed models to evaluate trajectories of change in traits, and estimates phenotypes to identify age-varying genetic effects in GWAS. Using childhood BMI as an example trait, we included 71,336 participants from six cohorts and estimated the slope and area under the BMI curve within four time periods (infancy, early childhood, late childhood and adolescence) for each participant, in addition to the age and BMI at the adiposity peak and the adiposity rebound. GWAS of the 12 estimated phenotypes identified 28 genome-wide significant variants at 13 loci, one of which (in DAOA) has not been previously associated with childhood or adult BMI. Genetic studies of changes in human traits over time could uncover unique biological mechanisms influencing quantitative traits.
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spelling doaj-art-28fab4a6a11341f3a0e4eff51ab735a72025-08-20T02:33:06ZengNature PortfolioNature Communications2041-17232024-11-0115111810.1038/s41467-024-53687-3A framework for conducting GWAS using repeated measures data with an application to childhood BMIKimberley Burrows0Anni Heiskala1Jonathan P. Bradfield2Zhanna Balkhiyarova3Lijiao Ning4Mathilde Boissel5Yee-Ming Chan6Philippe Froguel7Amelie Bonnefond8Hakon Hakonarson9Alexessander Couto Alves10Deborah A. Lawlor11Marika Kaakinen12Marjo-Riitta Järvelin13Struan F. A. Grant14Kate Tilling15Inga Prokopenko16Sylvain Sebert17Mickaël Canouil18Nicole M. Warrington19MRC Integrative Epidemiology Unit at the University of BristolResearch Unit of Population Health, University of OuluCenter for Applied Genomics, Children’s Hospital of PhiladelphiaDepartment of Clinical and Experimental Medicine, School of Biosciences, University of SurreyUniv Lille, INSERM/CNRS UMR1283/8199, EGID, Institut Pasteur de Lille, Lille University HospitalUniv Lille, INSERM/CNRS UMR1283/8199, EGID, Institut Pasteur de Lille, Lille University HospitalDivision of Endocrinology, Department of Pediatrics, Boston Children’s HospitalUniv Lille, INSERM/CNRS UMR1283/8199, EGID, Institut Pasteur de Lille, Lille University HospitalUniv Lille, INSERM/CNRS UMR1283/8199, EGID, Institut Pasteur de Lille, Lille University HospitalCenter for Applied Genomics, Children’s Hospital of PhiladelphiaSchool of Biosciences and Medicine, University of SurreyMRC Integrative Epidemiology Unit at the University of BristolDepartment of Clinical and Experimental Medicine, School of Biosciences, University of SurreyResearch Unit of Population Health, University of OuluCenter for Applied Genomics, Children’s Hospital of PhiladelphiaMRC Integrative Epidemiology Unit at the University of BristolDepartment of Clinical and Experimental Medicine, School of Biosciences, University of SurreyResearch Unit of Population Health, University of OuluUniv Lille, INSERM/CNRS UMR1283/8199, EGID, Institut Pasteur de Lille, Lille University HospitalInstitute for Molecular Bioscience, University of QueenslandAbstract Genetic effects on changes in human traits over time are understudied and may have important pathophysiological impact. We propose a framework that enables data quality control, implements mixed models to evaluate trajectories of change in traits, and estimates phenotypes to identify age-varying genetic effects in GWAS. Using childhood BMI as an example trait, we included 71,336 participants from six cohorts and estimated the slope and area under the BMI curve within four time periods (infancy, early childhood, late childhood and adolescence) for each participant, in addition to the age and BMI at the adiposity peak and the adiposity rebound. GWAS of the 12 estimated phenotypes identified 28 genome-wide significant variants at 13 loci, one of which (in DAOA) has not been previously associated with childhood or adult BMI. Genetic studies of changes in human traits over time could uncover unique biological mechanisms influencing quantitative traits.https://doi.org/10.1038/s41467-024-53687-3
spellingShingle Kimberley Burrows
Anni Heiskala
Jonathan P. Bradfield
Zhanna Balkhiyarova
Lijiao Ning
Mathilde Boissel
Yee-Ming Chan
Philippe Froguel
Amelie Bonnefond
Hakon Hakonarson
Alexessander Couto Alves
Deborah A. Lawlor
Marika Kaakinen
Marjo-Riitta Järvelin
Struan F. A. Grant
Kate Tilling
Inga Prokopenko
Sylvain Sebert
Mickaël Canouil
Nicole M. Warrington
A framework for conducting GWAS using repeated measures data with an application to childhood BMI
Nature Communications
title A framework for conducting GWAS using repeated measures data with an application to childhood BMI
title_full A framework for conducting GWAS using repeated measures data with an application to childhood BMI
title_fullStr A framework for conducting GWAS using repeated measures data with an application to childhood BMI
title_full_unstemmed A framework for conducting GWAS using repeated measures data with an application to childhood BMI
title_short A framework for conducting GWAS using repeated measures data with an application to childhood BMI
title_sort framework for conducting gwas using repeated measures data with an application to childhood bmi
url https://doi.org/10.1038/s41467-024-53687-3
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