Bjerkandera adusta TM11 for the bioremediation of fluoroquinolone antibiotics spiked in wastewater: A sustainable approach to pharmaceutical contaminant biotransformation

Global antibiotic consumption is increasing dramatically. Antibiotic release into the environment, primarily through wastewater discharge, has serious impacts for human and animal health and microbial ecosystems. To address this issue, white-rot fungi present a promising solution, as they possess ox...

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Main Authors: Hasna Befenzi, Amine Ezzariai, Jihane Baghor, Hamza Arrach, Jean Armengaud, Mélodie Kielbasa, Annick Doan, Julien Lambert, Anne Lomascolo, Quentin Albert, Craig B. Faulds, Giuliano Sciara, Tahar Mechichi, Lamfeddal Kouisni, Mohamed Hafidi, Loubna El Fels, Eric Record
Format: Article
Language:English
Published: Elsevier 2025-02-01
Series:Ecotoxicology and Environmental Safety
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Online Access:http://www.sciencedirect.com/science/article/pii/S0147651325002349
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author Hasna Befenzi
Amine Ezzariai
Jihane Baghor
Hamza Arrach
Jean Armengaud
Mélodie Kielbasa
Annick Doan
Julien Lambert
Anne Lomascolo
Quentin Albert
Craig B. Faulds
Giuliano Sciara
Tahar Mechichi
Lamfeddal Kouisni
Mohamed Hafidi
Loubna El Fels
Eric Record
author_facet Hasna Befenzi
Amine Ezzariai
Jihane Baghor
Hamza Arrach
Jean Armengaud
Mélodie Kielbasa
Annick Doan
Julien Lambert
Anne Lomascolo
Quentin Albert
Craig B. Faulds
Giuliano Sciara
Tahar Mechichi
Lamfeddal Kouisni
Mohamed Hafidi
Loubna El Fels
Eric Record
author_sort Hasna Befenzi
collection DOAJ
description Global antibiotic consumption is increasing dramatically. Antibiotic release into the environment, primarily through wastewater discharge, has serious impacts for human and animal health and microbial ecosystems. To address this issue, white-rot fungi present a promising solution, as they possess oxidative enzymes that can degrade these pollutants. Here we investigated the effectiveness of the white-rot fungus Bjerkandera adusta TM11 for removing three persistent fluoroquinolone antibiotics, i.e. levofloxacin, ciprofloxacin, and enrofloxacin, in real wastewater. The three antibiotics were added to the wastewater separately at a concentration of 30 mg/L and together in a cocktail at 10 mg/L, then incubated for 9 days. LC-MS/MS analysis and anti-microbial assay (against Escherichia coli) demonstrated complete removal of levofloxacin by day 7. However, ciprofloxacin and enrofloxacin biotransformed into degradation products that still had antimicrobial activity, with degradation efficiencies reaching 82 % and 99 %, respectively, by day 7. Proteomic analysis identified 21 fungal heme peroxidases. Versatile peroxidase was the most strongly-produced enzyme potentially involved in antibiotic biotransformation. Degradation products were characterized by LC-MS/MS analysis, and a degradation pathway was proposed based on these findings.
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spelling doaj-art-28f289945dba4f9387503b6636b5c62d2025-08-20T03:04:59ZengElsevierEcotoxicology and Environmental Safety0147-65132025-02-0129111789810.1016/j.ecoenv.2025.117898Bjerkandera adusta TM11 for the bioremediation of fluoroquinolone antibiotics spiked in wastewater: A sustainable approach to pharmaceutical contaminant biotransformationHasna Befenzi0Amine Ezzariai1Jihane Baghor2Hamza Arrach3Jean Armengaud4Mélodie Kielbasa5Annick Doan6Julien Lambert7Anne Lomascolo8Quentin Albert9Craig B. Faulds10Giuliano Sciara11Tahar Mechichi12Lamfeddal Kouisni13Mohamed Hafidi14Loubna El Fels15Eric Record16Cadi Ayyad University, Laboratory of Microbial Biotechnologies, Agrosciences and Environment (BioMAgE), Labeled Research Unit CNRST No. 4, Faculty of Sciences Semlalia, Marrakesh, Morocco; INRAE, Aix Marseille Univ, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, FranceMohammed VI Polytechnic University (UM6P), African Sustainable Agriculture Research Institute (ASARI), Laayoun, MoroccoCadi Ayyad University, Laboratory of Microbial Biotechnologies, Agrosciences and Environment (BioMAgE), Labeled Research Unit CNRST No. 4, Faculty of Sciences Semlalia, Marrakesh, MoroccoMohammed VI Polytechnic University (UM6P), African Sustainable Agriculture Research Institute (ASARI), Laayoun, MoroccoUniversité Paris-Saclay, Département Médicaments et Technologies pour la Santé, CEA, INRAE, SPI, Bagnols-sur-Cèze 30200, FranceUniversité Paris-Saclay, Département Médicaments et Technologies pour la Santé, CEA, INRAE, SPI, Bagnols-sur-Cèze 30200, FranceINRAE, Aix Marseille Univ, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, FranceINRAE, Aix Marseille Univ, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, FranceAix Marseille Univ, INRAE, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, FranceAix Marseille Univ, INRAE, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, FranceAix Marseille Univ, INRAE, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, FranceINRAE, Aix Marseille Univ, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, FranceUniversité de Sfax, Ecole Nationale d’Ingénieurs de Sfax, Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Sfax 3038, TunisiaMohammed VI Polytechnic University (UM6P), African Sustainable Agriculture Research Institute (ASARI), Laayoun, MoroccoCadi Ayyad University, Laboratory of Microbial Biotechnologies, Agrosciences and Environment (BioMAgE), Labeled Research Unit CNRST No. 4, Faculty of Sciences Semlalia, Marrakesh, Morocco; Mohammed VI Polytechnic University (UM6P), African Sustainable Agriculture Research Institute (ASARI), Laayoun, MoroccoCadi Ayyad University, Laboratory of Microbial Biotechnologies, Agrosciences and Environment (BioMAgE), Labeled Research Unit CNRST No. 4, Faculty of Sciences Semlalia, Marrakesh, Morocco; Corresponding authors.INRAE, Aix Marseille Univ, BBF, Biodiversité et Biotechnologie Fongiques, Marseille, France; Corresponding authors.Global antibiotic consumption is increasing dramatically. Antibiotic release into the environment, primarily through wastewater discharge, has serious impacts for human and animal health and microbial ecosystems. To address this issue, white-rot fungi present a promising solution, as they possess oxidative enzymes that can degrade these pollutants. Here we investigated the effectiveness of the white-rot fungus Bjerkandera adusta TM11 for removing three persistent fluoroquinolone antibiotics, i.e. levofloxacin, ciprofloxacin, and enrofloxacin, in real wastewater. The three antibiotics were added to the wastewater separately at a concentration of 30 mg/L and together in a cocktail at 10 mg/L, then incubated for 9 days. LC-MS/MS analysis and anti-microbial assay (against Escherichia coli) demonstrated complete removal of levofloxacin by day 7. However, ciprofloxacin and enrofloxacin biotransformed into degradation products that still had antimicrobial activity, with degradation efficiencies reaching 82 % and 99 %, respectively, by day 7. Proteomic analysis identified 21 fungal heme peroxidases. Versatile peroxidase was the most strongly-produced enzyme potentially involved in antibiotic biotransformation. Degradation products were characterized by LC-MS/MS analysis, and a degradation pathway was proposed based on these findings.http://www.sciencedirect.com/science/article/pii/S0147651325002349Fluoroquinolone antibioticsWastewaterWhite-rot fungiBjerkandera adustaHeme peroxidasesDegradation products
spellingShingle Hasna Befenzi
Amine Ezzariai
Jihane Baghor
Hamza Arrach
Jean Armengaud
Mélodie Kielbasa
Annick Doan
Julien Lambert
Anne Lomascolo
Quentin Albert
Craig B. Faulds
Giuliano Sciara
Tahar Mechichi
Lamfeddal Kouisni
Mohamed Hafidi
Loubna El Fels
Eric Record
Bjerkandera adusta TM11 for the bioremediation of fluoroquinolone antibiotics spiked in wastewater: A sustainable approach to pharmaceutical contaminant biotransformation
Ecotoxicology and Environmental Safety
Fluoroquinolone antibiotics
Wastewater
White-rot fungi
Bjerkandera adusta
Heme peroxidases
Degradation products
title Bjerkandera adusta TM11 for the bioremediation of fluoroquinolone antibiotics spiked in wastewater: A sustainable approach to pharmaceutical contaminant biotransformation
title_full Bjerkandera adusta TM11 for the bioremediation of fluoroquinolone antibiotics spiked in wastewater: A sustainable approach to pharmaceutical contaminant biotransformation
title_fullStr Bjerkandera adusta TM11 for the bioremediation of fluoroquinolone antibiotics spiked in wastewater: A sustainable approach to pharmaceutical contaminant biotransformation
title_full_unstemmed Bjerkandera adusta TM11 for the bioremediation of fluoroquinolone antibiotics spiked in wastewater: A sustainable approach to pharmaceutical contaminant biotransformation
title_short Bjerkandera adusta TM11 for the bioremediation of fluoroquinolone antibiotics spiked in wastewater: A sustainable approach to pharmaceutical contaminant biotransformation
title_sort bjerkandera adusta tm11 for the bioremediation of fluoroquinolone antibiotics spiked in wastewater a sustainable approach to pharmaceutical contaminant biotransformation
topic Fluoroquinolone antibiotics
Wastewater
White-rot fungi
Bjerkandera adusta
Heme peroxidases
Degradation products
url http://www.sciencedirect.com/science/article/pii/S0147651325002349
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