IL-8 and MCP Gene Expression and Production by LPS-Stimulated Human Corneal Stromal Cells
Purpose. To determine time course of effect of lipopolysaccharide (LPS) on production of interleukin-8 (IL-8) and monocyte chemotactic protein (MCP) by cultured human corneal stromal cells. Methods. Human corneal stromal cells were harvested from donor corneal specimens, and fourth to sixth passaged...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/714704 |
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| _version_ | 1849307360728711168 |
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| author | Roni M. Shtein Susan G. Elner Zong-Mei Bian Victor M. Elner |
| author_facet | Roni M. Shtein Susan G. Elner Zong-Mei Bian Victor M. Elner |
| author_sort | Roni M. Shtein |
| collection | DOAJ |
| description | Purpose. To determine time course of effect of lipopolysaccharide (LPS) on production of interleukin-8 (IL-8) and monocyte chemotactic protein (MCP) by cultured human corneal stromal cells. Methods. Human corneal stromal cells were harvested from donor corneal specimens, and fourth to sixth passaged cells were used. Cell cultures were stimulated with LPS for 2, 4, 8, and 24 hours. Northern blot analysis of IL-8 and MCP gene expression and ELISA for IL-8 and MCP secretion were performed. ELISA results were analyzed for statistical significance using two-tailed Student's t-test. Results. Northern blot analysis demonstrated significantly increased IL-8 and MCP gene expression after 4 and 8 hours of exposure to LPS. ELISA for secreted IL-8 and MCP demonstrated statistically significant increases (P<0.05) after corneal stromal cell stimulation with LPS. Conclusions. This paper suggests that human corneal stromal cells may participate in corneal inflammation by secreting potent leukocyte chemotactic and activating proteins in a time-dependent manner when exposed to LPS. |
| format | Article |
| id | doaj-art-28efdfd22c4f464dbc66ea810cb931c0 |
| institution | Kabale University |
| issn | 2090-8040 2042-0099 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Inflammation |
| spelling | doaj-art-28efdfd22c4f464dbc66ea810cb931c02025-08-20T03:54:47ZengWileyInternational Journal of Inflammation2090-80402042-00992012-01-01201210.1155/2012/714704714704IL-8 and MCP Gene Expression and Production by LPS-Stimulated Human Corneal Stromal CellsRoni M. Shtein0Susan G. Elner1Zong-Mei Bian2Victor M. Elner3Department of Ophthalmology and Visual Sciences, University of Michigan Kellogg Eye Center, 1000 Wall Street, Ann Arbor, MI 48105, USADepartment of Ophthalmology and Visual Sciences, University of Michigan Kellogg Eye Center, 1000 Wall Street, Ann Arbor, MI 48105, USADepartment of Ophthalmology and Visual Sciences, University of Michigan Kellogg Eye Center, 1000 Wall Street, Ann Arbor, MI 48105, USADepartment of Ophthalmology and Visual Sciences, University of Michigan Kellogg Eye Center, 1000 Wall Street, Ann Arbor, MI 48105, USAPurpose. To determine time course of effect of lipopolysaccharide (LPS) on production of interleukin-8 (IL-8) and monocyte chemotactic protein (MCP) by cultured human corneal stromal cells. Methods. Human corneal stromal cells were harvested from donor corneal specimens, and fourth to sixth passaged cells were used. Cell cultures were stimulated with LPS for 2, 4, 8, and 24 hours. Northern blot analysis of IL-8 and MCP gene expression and ELISA for IL-8 and MCP secretion were performed. ELISA results were analyzed for statistical significance using two-tailed Student's t-test. Results. Northern blot analysis demonstrated significantly increased IL-8 and MCP gene expression after 4 and 8 hours of exposure to LPS. ELISA for secreted IL-8 and MCP demonstrated statistically significant increases (P<0.05) after corneal stromal cell stimulation with LPS. Conclusions. This paper suggests that human corneal stromal cells may participate in corneal inflammation by secreting potent leukocyte chemotactic and activating proteins in a time-dependent manner when exposed to LPS.http://dx.doi.org/10.1155/2012/714704 |
| spellingShingle | Roni M. Shtein Susan G. Elner Zong-Mei Bian Victor M. Elner IL-8 and MCP Gene Expression and Production by LPS-Stimulated Human Corneal Stromal Cells International Journal of Inflammation |
| title | IL-8 and MCP Gene Expression and Production by LPS-Stimulated Human Corneal Stromal Cells |
| title_full | IL-8 and MCP Gene Expression and Production by LPS-Stimulated Human Corneal Stromal Cells |
| title_fullStr | IL-8 and MCP Gene Expression and Production by LPS-Stimulated Human Corneal Stromal Cells |
| title_full_unstemmed | IL-8 and MCP Gene Expression and Production by LPS-Stimulated Human Corneal Stromal Cells |
| title_short | IL-8 and MCP Gene Expression and Production by LPS-Stimulated Human Corneal Stromal Cells |
| title_sort | il 8 and mcp gene expression and production by lps stimulated human corneal stromal cells |
| url | http://dx.doi.org/10.1155/2012/714704 |
| work_keys_str_mv | AT ronimshtein il8andmcpgeneexpressionandproductionbylpsstimulatedhumancornealstromalcells AT susangelner il8andmcpgeneexpressionandproductionbylpsstimulatedhumancornealstromalcells AT zongmeibian il8andmcpgeneexpressionandproductionbylpsstimulatedhumancornealstromalcells AT victormelner il8andmcpgeneexpressionandproductionbylpsstimulatedhumancornealstromalcells |