Mesenchymal stem cells <i>ex vivo</i> cultivation technologies for clinical use
Now mesenchymal stem cells (MSC) are more widely applied in different areas of medicine. However, despite requirement for considerable quantities of human MSC for clinical practice, there is restricted information concerning culture conditions optimization required for their production. In present a...
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| Format: | Article |
| Language: | Russian |
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ABV-press
2022-11-01
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| Series: | Онкогематология |
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| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/779 |
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| _version_ | 1849241312981680128 |
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| author | T. V. Shamanskaya E. Yu. Osipova S. A. Roumiantsev |
| author_facet | T. V. Shamanskaya E. Yu. Osipova S. A. Roumiantsev |
| author_sort | T. V. Shamanskaya |
| collection | DOAJ |
| description | Now mesenchymal stem cells (MSC) are more widely applied in different areas of medicine. However, despite requirement for considerable quantities of human MSC for clinical practice, there is restricted information concerning culture conditions optimization required for their production. In present article the literature review, concerning various conditions of MSC extraction and culture, is presented. Influence of culture medium, cells passage density, fetal calf serum presence or absence and other conditions on MSC proliferation is surveyed. Data concerning use of three-dimensional structures and various bioreactors for ex vivo expansion are presented. MSC multilinear differentiation features are surveyed. |
| format | Article |
| id | doaj-art-28ef7af22d0142259f0c0206524014aa |
| institution | Kabale University |
| issn | 1818-8346 2413-4023 |
| language | Russian |
| publishDate | 2022-11-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Онкогематология |
| spelling | doaj-art-28ef7af22d0142259f0c0206524014aa2025-08-20T04:00:13ZrusABV-pressОнкогематология1818-83462413-40232022-11-0103697610.17650/1818-8346-2009-0-3-69-76657Mesenchymal stem cells <i>ex vivo</i> cultivation technologies for clinical useT. V. Shamanskaya0E. Yu. Osipova1S. A. Roumiantsev2Federal Research Center of Pediatric Hematology, Oncology and Immunology; Moscow Stem Cells BankFederal Research Center of Pediatric Hematology, Oncology and Immunology; Moscow Stem Cells BankFederal Research Center of Pediatric Hematology, Oncology and Immunology; Moscow Stem Cells BankNow mesenchymal stem cells (MSC) are more widely applied in different areas of medicine. However, despite requirement for considerable quantities of human MSC for clinical practice, there is restricted information concerning culture conditions optimization required for their production. In present article the literature review, concerning various conditions of MSC extraction and culture, is presented. Influence of culture medium, cells passage density, fetal calf serum presence or absence and other conditions on MSC proliferation is surveyed. Data concerning use of three-dimensional structures and various bioreactors for ex vivo expansion are presented. MSC multilinear differentiation features are surveyed.https://oncohematology.abvpress.ru/ongm/article/view/779mesenchymal stem cells<i>ex vivo</i> expansiondifferentiationcell cultures |
| spellingShingle | T. V. Shamanskaya E. Yu. Osipova S. A. Roumiantsev Mesenchymal stem cells <i>ex vivo</i> cultivation technologies for clinical use Онкогематология mesenchymal stem cells <i>ex vivo</i> expansion differentiation cell cultures |
| title | Mesenchymal stem cells <i>ex vivo</i> cultivation technologies for clinical use |
| title_full | Mesenchymal stem cells <i>ex vivo</i> cultivation technologies for clinical use |
| title_fullStr | Mesenchymal stem cells <i>ex vivo</i> cultivation technologies for clinical use |
| title_full_unstemmed | Mesenchymal stem cells <i>ex vivo</i> cultivation technologies for clinical use |
| title_short | Mesenchymal stem cells <i>ex vivo</i> cultivation technologies for clinical use |
| title_sort | mesenchymal stem cells i ex vivo i cultivation technologies for clinical use |
| topic | mesenchymal stem cells <i>ex vivo</i> expansion differentiation cell cultures |
| url | https://oncohematology.abvpress.ru/ongm/article/view/779 |
| work_keys_str_mv | AT tvshamanskaya mesenchymalstemcellsiexvivoicultivationtechnologiesforclinicaluse AT eyuosipova mesenchymalstemcellsiexvivoicultivationtechnologiesforclinicaluse AT saroumiantsev mesenchymalstemcellsiexvivoicultivationtechnologiesforclinicaluse |