Development and Validation of a Method for the Quantification of an Alkaloid Fraction of Himatanthus lancifolius (Muell. Arg.) Woodson by Ultraviolet Spectroscopy
For the registration of phytomedicines and their availability to the population, National Agency of Sanitary Surveillance (ANVISA) establishes quality, security, and efficacy parameters, stipulating control requirements similar to those applied to synthetic medicines. This work reports the investiga...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/652392 |
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| author | Patrícia M. S. S. Barros Nádia M. G. de Couto Andressa S. B. Silva Wagner L. R. Barbosa |
| author_facet | Patrícia M. S. S. Barros Nádia M. G. de Couto Andressa S. B. Silva Wagner L. R. Barbosa |
| author_sort | Patrícia M. S. S. Barros |
| collection | DOAJ |
| description | For the registration of phytomedicines and their availability to the population, National Agency of Sanitary Surveillance (ANVISA) establishes quality, security, and efficacy parameters, stipulating control requirements similar to those applied to synthetic medicines. This work reports the investigation of the bark of Himatanthus lancifolius and its extracts aiming to contribute to the standardization of derivatives of this plant species. The developed quantification method shows high selectivity at 281nm, which confers confidence to the detection of the alkaloids. The method is robust, according to the current regulation, and shows linearity, precision, and accuracy, beside accessibility and simplicity in execute. The pH 10 alkaloid fraction obtained from the aqueous extract of the analyzed sample represents 0.219% in the dried extract. These results contribute for reducing the lack of methods for the quality control of phytomedicines prepared from H. lancifolius. |
| format | Article |
| id | doaj-art-28eb615e21f640ceafbb25b5f382d935 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-28eb615e21f640ceafbb25b5f382d9352025-08-20T03:54:47ZengWileyJournal of Chemistry2090-90632090-90712013-01-01201310.1155/2013/652392652392Development and Validation of a Method for the Quantification of an Alkaloid Fraction of Himatanthus lancifolius (Muell. Arg.) Woodson by Ultraviolet SpectroscopyPatrícia M. S. S. Barros0Nádia M. G. de Couto1Andressa S. B. Silva2Wagner L. R. Barbosa3Programa de Pós-Graduação em Ciências Farmacêuticas, Instituto de Ciências da Saúde, Universidade Federal do Pará, BrazilLaboratório de Fitoquímica, Faculdade de Farmácia, Instituto de Ciências da Saúde, Universidade Federal do Pará, BrazilPrograma de Pós-Graduação em Inovação Farmacêutica, Instituto de Ciências da Saúde, Universidade Federal do Pará, Rua Augusto Corrêa No. 01, Guamá, 66075-170 Belém, PA, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Instituto de Ciências da Saúde, Universidade Federal do Pará, BrazilFor the registration of phytomedicines and their availability to the population, National Agency of Sanitary Surveillance (ANVISA) establishes quality, security, and efficacy parameters, stipulating control requirements similar to those applied to synthetic medicines. This work reports the investigation of the bark of Himatanthus lancifolius and its extracts aiming to contribute to the standardization of derivatives of this plant species. The developed quantification method shows high selectivity at 281nm, which confers confidence to the detection of the alkaloids. The method is robust, according to the current regulation, and shows linearity, precision, and accuracy, beside accessibility and simplicity in execute. The pH 10 alkaloid fraction obtained from the aqueous extract of the analyzed sample represents 0.219% in the dried extract. These results contribute for reducing the lack of methods for the quality control of phytomedicines prepared from H. lancifolius.http://dx.doi.org/10.1155/2013/652392 |
| spellingShingle | Patrícia M. S. S. Barros Nádia M. G. de Couto Andressa S. B. Silva Wagner L. R. Barbosa Development and Validation of a Method for the Quantification of an Alkaloid Fraction of Himatanthus lancifolius (Muell. Arg.) Woodson by Ultraviolet Spectroscopy Journal of Chemistry |
| title | Development and Validation of a Method for the Quantification of an Alkaloid Fraction of Himatanthus lancifolius (Muell. Arg.)
Woodson by Ultraviolet Spectroscopy |
| title_full | Development and Validation of a Method for the Quantification of an Alkaloid Fraction of Himatanthus lancifolius (Muell. Arg.)
Woodson by Ultraviolet Spectroscopy |
| title_fullStr | Development and Validation of a Method for the Quantification of an Alkaloid Fraction of Himatanthus lancifolius (Muell. Arg.)
Woodson by Ultraviolet Spectroscopy |
| title_full_unstemmed | Development and Validation of a Method for the Quantification of an Alkaloid Fraction of Himatanthus lancifolius (Muell. Arg.)
Woodson by Ultraviolet Spectroscopy |
| title_short | Development and Validation of a Method for the Quantification of an Alkaloid Fraction of Himatanthus lancifolius (Muell. Arg.)
Woodson by Ultraviolet Spectroscopy |
| title_sort | development and validation of a method for the quantification of an alkaloid fraction of himatanthus lancifolius muell arg woodson by ultraviolet spectroscopy |
| url | http://dx.doi.org/10.1155/2013/652392 |
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