Alleviating the IL-1β-stimulated extracellular matrix degradation in osteoarthritis, and chondrocyte inflammation by Morinda officinalis polysaccharide via the SIRT6/NF-κB pathway
Morinda officinalis polysaccharide (MOP) is a major active component of Morinda officinalis, known for its roles in supporting bone health and reducing oxidation and inflammation. However, no studies to date have specifically examined the effects of MOP on interleukin-1β (IL-1β)-stimulated chondroc...
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2025-01-01
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| Series: | Biomolecules & Biomedicine |
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| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/11437 |
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| author | Dongfang Zhao Shuqin Xing Jiao Qi Zhiqiang Wei Jianghai Huang Jigao Sun Xinzhu Wen Yafei Wang |
| author_facet | Dongfang Zhao Shuqin Xing Jiao Qi Zhiqiang Wei Jianghai Huang Jigao Sun Xinzhu Wen Yafei Wang |
| author_sort | Dongfang Zhao |
| collection | DOAJ |
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Morinda officinalis polysaccharide (MOP) is a major active component of Morinda officinalis, known for its roles in supporting bone health and reducing oxidation and inflammation. However, no studies to date have specifically examined the effects of MOP on interleukin-1β (IL-1β)-stimulated chondrocyte inflammation or the progression of osteoarthritis (OA). To investigate, CCK-8 (cell counting kit-8) assays were performed to evaluate MOP’s impact on the viability of human chondrocytes (C28/I2 cells). Cell damage was assessed using flow cytometry and Hoechst 33258 fluorescent staining. Inflammatory factor levels were measured via western blot and ELISA, while extracellular matrix (ECM) degradation was analyzed through immunofluorescence. The involvement of the NF-κB pathway and its regulation by SIRT6 were also explored using western blot. Following IL-1β treatment, C28/I2 cell viability decreased, inflammatory factor secretion increased, and ECM degradation was observed. MOP counteracted these effects by mitigating IL-1β-induced cell damage, preventing ECM degradation, and reducing inflammatory factor secretion, in a dose-dependent manner. Furthermore, IL-1β treatment suppressed SIRT6 expression, whereas MOP upregulated it. Notably, silencing SIRT6 diminished MOP’s protective effects on C28/I2 cells and reversed MOP’s suppression of the NF-κB pathway.In conclusion, MOP alleviates IL-1β-induced C28/I2 cell injury by inhibiting the NF-κB pathway through activation of SIRT6. This, in turn, reduces the inflammatory response, prevents ECM degradation, and ultimately slows OA progression.
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| format | Article |
| id | doaj-art-28e83e695498497f83c37ee91019b92d |
| institution | Kabale University |
| issn | 2831-0896 2831-090X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj-art-28e83e695498497f83c37ee91019b92d2025-01-09T16:39:08ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2025-01-0110.17305/bb.2024.11437Alleviating the IL-1β-stimulated extracellular matrix degradation in osteoarthritis, and chondrocyte inflammation by Morinda officinalis polysaccharide via the SIRT6/NF-κB pathwayDongfang Zhao0Shuqin Xing1Jiao Qi2Zhiqiang Wei3Jianghai Huang4Jigao Sun5Xinzhu Wen6Yafei Wang7Department of Orthopaedics, Dongfang Hospital, Beijing University of Chinese Medicine, ChinaDepartment of Oncology, Dongfang Hospital, Beijing University of Chinese Medicine, ChinaNursing in the South Hospital of Dongfang Hospital, Beijing University of Chinese Medicine, ChinaDepartment of Orthopaedics, Dongfang Hospital, Beijing University of Chinese Medicine, ChinaDepartment of Orthopaedics, Dongfang Hospital, Beijing University of Chinese Medicine, ChinaDepartment of Orthopaedics, Dongfang Hospital, Beijing University of Chinese Medicine, ChinaDepartment of Orthopaedics, Dongfang Hospital, Beijing University of Chinese Medicine, ChinaDepartment of Orthopaedics, Dongfang Hospital, Beijing University of Chinese Medicine, China Morinda officinalis polysaccharide (MOP) is a major active component of Morinda officinalis, known for its roles in supporting bone health and reducing oxidation and inflammation. However, no studies to date have specifically examined the effects of MOP on interleukin-1β (IL-1β)-stimulated chondrocyte inflammation or the progression of osteoarthritis (OA). To investigate, CCK-8 (cell counting kit-8) assays were performed to evaluate MOP’s impact on the viability of human chondrocytes (C28/I2 cells). Cell damage was assessed using flow cytometry and Hoechst 33258 fluorescent staining. Inflammatory factor levels were measured via western blot and ELISA, while extracellular matrix (ECM) degradation was analyzed through immunofluorescence. The involvement of the NF-κB pathway and its regulation by SIRT6 were also explored using western blot. Following IL-1β treatment, C28/I2 cell viability decreased, inflammatory factor secretion increased, and ECM degradation was observed. MOP counteracted these effects by mitigating IL-1β-induced cell damage, preventing ECM degradation, and reducing inflammatory factor secretion, in a dose-dependent manner. Furthermore, IL-1β treatment suppressed SIRT6 expression, whereas MOP upregulated it. Notably, silencing SIRT6 diminished MOP’s protective effects on C28/I2 cells and reversed MOP’s suppression of the NF-κB pathway.In conclusion, MOP alleviates IL-1β-induced C28/I2 cell injury by inhibiting the NF-κB pathway through activation of SIRT6. This, in turn, reduces the inflammatory response, prevents ECM degradation, and ultimately slows OA progression. https://www.bjbms.org/ojs/index.php/bjbms/article/view/11437Morinda officinalis polysaccharidesMOPosteoarthritisOAsirtuin 6SIRT6 |
| spellingShingle | Dongfang Zhao Shuqin Xing Jiao Qi Zhiqiang Wei Jianghai Huang Jigao Sun Xinzhu Wen Yafei Wang Alleviating the IL-1β-stimulated extracellular matrix degradation in osteoarthritis, and chondrocyte inflammation by Morinda officinalis polysaccharide via the SIRT6/NF-κB pathway Biomolecules & Biomedicine Morinda officinalis polysaccharides MOP osteoarthritis OA sirtuin 6 SIRT6 |
| title | Alleviating the IL-1β-stimulated extracellular matrix degradation in osteoarthritis, and chondrocyte inflammation by Morinda officinalis polysaccharide via the SIRT6/NF-κB pathway |
| title_full | Alleviating the IL-1β-stimulated extracellular matrix degradation in osteoarthritis, and chondrocyte inflammation by Morinda officinalis polysaccharide via the SIRT6/NF-κB pathway |
| title_fullStr | Alleviating the IL-1β-stimulated extracellular matrix degradation in osteoarthritis, and chondrocyte inflammation by Morinda officinalis polysaccharide via the SIRT6/NF-κB pathway |
| title_full_unstemmed | Alleviating the IL-1β-stimulated extracellular matrix degradation in osteoarthritis, and chondrocyte inflammation by Morinda officinalis polysaccharide via the SIRT6/NF-κB pathway |
| title_short | Alleviating the IL-1β-stimulated extracellular matrix degradation in osteoarthritis, and chondrocyte inflammation by Morinda officinalis polysaccharide via the SIRT6/NF-κB pathway |
| title_sort | alleviating the il 1β stimulated extracellular matrix degradation in osteoarthritis and chondrocyte inflammation by morinda officinalis polysaccharide via the sirt6 nf κb pathway |
| topic | Morinda officinalis polysaccharides MOP osteoarthritis OA sirtuin 6 SIRT6 |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/11437 |
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