Extraction process optimization for maximizing total polyphenol content in cashew nut testa (Anacardium occidentale L.) extract

Cashew nut testa (Anacardium occidentale L.) is a valuable source of polyphenols known for their strong bioactivities. This study optimized the extraction conditions to obtain polyphenol-rich extracts from cashew nut testa. The effects of three key factors, extraction time, material-to-solvent ratio...

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Main Authors: Dung Thi Nguyen, Toan Van Nguyen, Tu Le Kha Bui, Lieu Thi Nguyen, Son Hai Pham, Trinh Thi Nu Nguyen, Trang Thi Thuy Nguyen, Thinh Bao Bui
Format: Article
Language:English
Published: HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE 2025-01-01
Series:Ho Chi Minh City Open University Journal of Science - Engineering and Technology
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Online Access:https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/3786
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author Dung Thi Nguyen
Toan Van Nguyen
Tu Le Kha Bui
Lieu Thi Nguyen
Son Hai Pham
Trinh Thi Nu Nguyen
Trang Thi Thuy Nguyen
Thinh Bao Bui
author_facet Dung Thi Nguyen
Toan Van Nguyen
Tu Le Kha Bui
Lieu Thi Nguyen
Son Hai Pham
Trinh Thi Nu Nguyen
Trang Thi Thuy Nguyen
Thinh Bao Bui
author_sort Dung Thi Nguyen
collection DOAJ
description Cashew nut testa (Anacardium occidentale L.) is a valuable source of polyphenols known for their strong bioactivities. This study optimized the extraction conditions to obtain polyphenol-rich extracts from cashew nut testa. The effects of three key factors, extraction time, material-to-solvent ratio, and temperature, on Total Polyphenol Content (TPC, mg GAE/g extract) were investigated. Response surface methodology, using a Box-Behnken design, was applied to design the experiments and optimize the extraction process. The results showed that the relationship between TPC and extraction conditions followed a second-order model with an R² value of 0.9999. All three factors significantly influenced TPC (p < 0.05), and their interactions were also significant. The model predicted optimal conditions for maximum TPC at an extraction time of 19.85h, a temperature of 58.80°C, and a material-to-solvent ratio of 1:16.39 (w/v). Validation experiments under the optimized conditions of 20h, 59°C, and a 1:16 ratio confirmed the model's accuracy, yielding a TPC of 534.67mg GAE/g extract, statistically equivalent to the predicted value. These findings demonstrate the efficacy of the optimized conditions in maximizing polyphenol extraction from cashew nut testa.
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publishDate 2025-01-01
publisher HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE
record_format Article
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spelling doaj-art-28de0d43728f4495a16870c10e81e2d22025-08-20T02:16:04ZengHO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCEHo Chi Minh City Open University Journal of Science - Engineering and Technology2734-93302734-96082025-01-01151344610.46223/HCMCOUJS.tech.en.15.1.3786.20252322Extraction process optimization for maximizing total polyphenol content in cashew nut testa (Anacardium occidentale L.) extractDung Thi Nguyen0Toan Van Nguyen1Tu Le Kha Bui2Lieu Thi Nguyen3Son Hai Pham4Trinh Thi Nu Nguyen5Trang Thi Thuy Nguyen6Thinh Bao Bui7Biotechnology Center of Ho Chi Minh City, Ho Chi Minh CityBiotechnology Center of Ho Chi Minh City, Ho Chi Minh CityBiotechnology Center of Ho Chi Minh City, Ho Chi Minh CityBiotechnology Center of Ho Chi Minh City, Ho Chi Minh CityBiotechnology Center of Ho Chi Minh City, Ho Chi Minh CityBiotechnology Center of Ho Chi Minh City, Ho Chi Minh CityBiotechnology Center of Ho Chi Minh City, Ho Chi Minh CityBiotechnology Center of Ho Chi Minh City, Ho Chi Minh CityCashew nut testa (Anacardium occidentale L.) is a valuable source of polyphenols known for their strong bioactivities. This study optimized the extraction conditions to obtain polyphenol-rich extracts from cashew nut testa. The effects of three key factors, extraction time, material-to-solvent ratio, and temperature, on Total Polyphenol Content (TPC, mg GAE/g extract) were investigated. Response surface methodology, using a Box-Behnken design, was applied to design the experiments and optimize the extraction process. The results showed that the relationship between TPC and extraction conditions followed a second-order model with an R² value of 0.9999. All three factors significantly influenced TPC (p < 0.05), and their interactions were also significant. The model predicted optimal conditions for maximum TPC at an extraction time of 19.85h, a temperature of 58.80°C, and a material-to-solvent ratio of 1:16.39 (w/v). Validation experiments under the optimized conditions of 20h, 59°C, and a 1:16 ratio confirmed the model's accuracy, yielding a TPC of 534.67mg GAE/g extract, statistically equivalent to the predicted value. These findings demonstrate the efficacy of the optimized conditions in maximizing polyphenol extraction from cashew nut testa.https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/3786anacardium occidentalecashew nut testaextraction conditionspolyphenolresponse surface methodology
spellingShingle Dung Thi Nguyen
Toan Van Nguyen
Tu Le Kha Bui
Lieu Thi Nguyen
Son Hai Pham
Trinh Thi Nu Nguyen
Trang Thi Thuy Nguyen
Thinh Bao Bui
Extraction process optimization for maximizing total polyphenol content in cashew nut testa (Anacardium occidentale L.) extract
Ho Chi Minh City Open University Journal of Science - Engineering and Technology
anacardium occidentale
cashew nut testa
extraction conditions
polyphenol
response surface methodology
title Extraction process optimization for maximizing total polyphenol content in cashew nut testa (Anacardium occidentale L.) extract
title_full Extraction process optimization for maximizing total polyphenol content in cashew nut testa (Anacardium occidentale L.) extract
title_fullStr Extraction process optimization for maximizing total polyphenol content in cashew nut testa (Anacardium occidentale L.) extract
title_full_unstemmed Extraction process optimization for maximizing total polyphenol content in cashew nut testa (Anacardium occidentale L.) extract
title_short Extraction process optimization for maximizing total polyphenol content in cashew nut testa (Anacardium occidentale L.) extract
title_sort extraction process optimization for maximizing total polyphenol content in cashew nut testa anacardium occidentale l extract
topic anacardium occidentale
cashew nut testa
extraction conditions
polyphenol
response surface methodology
url https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/3786
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