Droplet digital PCR assay for precise determination of FRS2 gene copy number in bladder cancer

Droplet digital PCR assay for precise determination of FRS2 gene copy number in bladder cancer

Abstract Objective To establish and validate a droplet digital PCR (ddPCR) assay for quantifying FRS2 gene copy number in formalin-fixed paraffin-embedded (FFPE) bladder cancer tissue samples, and to evaluate its analytical performance. Methods The ddPCR assay was developed using FRS2 as the target...

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Bibliographic Details
Main Authors: Jinqian Li, Jiaqi Liang, Yinyan Xu, Wei Tan, Guanzheng Chen, Tong Ou
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Cancer
Subjects:
Bladder cancer
Gene copy number
Formalin-fixed paraffin-embedded (FFPE)
FRS2
Droplet digital PCR (ddPCR)
Online Access:https://doi.org/10.1186/s12885-025-14611-0
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