Effect of Robusta Coffee Bean Extract as Immunoregulator on Dental Pulp Mesenchymal Stem Cells via Paracrine Pathway
Objective: To analyze the effect of Robusta Coffee Bean Extract (RCBE) on Dental Pulp Mesenchymal Stem Cells (DPSCs) culture in secreting cytokines, growth factors, and cell differentiation. Material and Methods: DPSCs culture from premolar human teeth only and DPSCs culture from premolar human tee...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Association of Support to Oral Health Research (APESB)
2025-02-01
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| Series: | Pesquisa Brasileira em Odontopediatria e Clínica Integrada |
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| Online Access: | https://revista.uepb.edu.br/PBOCI/article/view/4290 |
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| author | Desi Sandra Sari Millenieo Martinindo Syafika Nuring Fadiyah Indah Yulia Ningsih Tantin Ermawati Tantin Ermawati Zahara Meilawaty Dessy Rachmawati Ernie Maduratna Fedik Abdul Rantam |
| author_facet | Desi Sandra Sari Millenieo Martinindo Syafika Nuring Fadiyah Indah Yulia Ningsih Tantin Ermawati Tantin Ermawati Zahara Meilawaty Dessy Rachmawati Ernie Maduratna Fedik Abdul Rantam |
| author_sort | Desi Sandra Sari |
| collection | DOAJ |
| description | Objective: To analyze the effect of Robusta Coffee Bean Extract (RCBE) on Dental Pulp Mesenchymal Stem Cells (DPSCs) culture in secreting cytokines, growth factors, and cell differentiation. Material and Methods: DPSCs culture from premolar human teeth only and DPSCs culture from premolar human teeth given RCBE with concentrations of 0.0625%, 0.125%, 0.25%, and 0.5% for 24 hours, 48 hours, and 72 hours. The secretome of the DPSCs culture was examined for TNF-α, IFN-g, IGF, and VEGF, examination of SOX2 and Oct4, and Wnt differentiation markers by ELISA. Statistical analysis used ANOVA and continued with LSD. Results: There was a significant reduction in the levels of TNF-α and IFN-g at 0.25% RCBE concentration at 72 hours of immersion (p<0.05). Growth factor levels of IGF and VEGF increased when given 0.25% RCBE compared to the other groups, and the differentiation markers SOX2 and Oct4 and Wnt also increased at a concentration of 0.25% at 72 hours of immersion (p<0.05). Conclusion: Giving RCBE with a concentration of 0.25% can reduce inflammatory cytokines and increase growth factors and differentiation markers in DPSCs cultures. |
| format | Article |
| id | doaj-art-28c387a299ff42429baae663dd8a2247 |
| institution | Kabale University |
| issn | 1519-0501 1983-4632 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Association of Support to Oral Health Research (APESB) |
| record_format | Article |
| series | Pesquisa Brasileira em Odontopediatria e Clínica Integrada |
| spelling | doaj-art-28c387a299ff42429baae663dd8a22472025-02-08T10:26:54ZengAssociation of Support to Oral Health Research (APESB)Pesquisa Brasileira em Odontopediatria e Clínica Integrada1519-05011983-46322025-02-0125Effect of Robusta Coffee Bean Extract as Immunoregulator on Dental Pulp Mesenchymal Stem Cells via Paracrine PathwayDesi Sandra SariMillenieo MartinindoSyafika Nuring FadiyahIndah Yulia NingsihTantin Ermawati Tantin ErmawatiZahara MeilawatyDessy RachmawatiErnie MaduratnaFedik Abdul RantamObjective: To analyze the effect of Robusta Coffee Bean Extract (RCBE) on Dental Pulp Mesenchymal Stem Cells (DPSCs) culture in secreting cytokines, growth factors, and cell differentiation. Material and Methods: DPSCs culture from premolar human teeth only and DPSCs culture from premolar human teeth given RCBE with concentrations of 0.0625%, 0.125%, 0.25%, and 0.5% for 24 hours, 48 hours, and 72 hours. The secretome of the DPSCs culture was examined for TNF-α, IFN-g, IGF, and VEGF, examination of SOX2 and Oct4, and Wnt differentiation markers by ELISA. Statistical analysis used ANOVA and continued with LSD. Results: There was a significant reduction in the levels of TNF-α and IFN-g at 0.25% RCBE concentration at 72 hours of immersion (p<0.05). Growth factor levels of IGF and VEGF increased when given 0.25% RCBE compared to the other groups, and the differentiation markers SOX2 and Oct4 and Wnt also increased at a concentration of 0.25% at 72 hours of immersion (p<0.05). Conclusion: Giving RCBE with a concentration of 0.25% can reduce inflammatory cytokines and increase growth factors and differentiation markers in DPSCs cultures.https://revista.uepb.edu.br/PBOCI/article/view/4290Dental PulpMesenchymal Stem CellsSecretomeHerbal |
| spellingShingle | Desi Sandra Sari Millenieo Martinindo Syafika Nuring Fadiyah Indah Yulia Ningsih Tantin Ermawati Tantin Ermawati Zahara Meilawaty Dessy Rachmawati Ernie Maduratna Fedik Abdul Rantam Effect of Robusta Coffee Bean Extract as Immunoregulator on Dental Pulp Mesenchymal Stem Cells via Paracrine Pathway Pesquisa Brasileira em Odontopediatria e Clínica Integrada Dental Pulp Mesenchymal Stem Cells Secretome Herbal |
| title | Effect of Robusta Coffee Bean Extract as Immunoregulator on Dental Pulp Mesenchymal Stem Cells via Paracrine Pathway |
| title_full | Effect of Robusta Coffee Bean Extract as Immunoregulator on Dental Pulp Mesenchymal Stem Cells via Paracrine Pathway |
| title_fullStr | Effect of Robusta Coffee Bean Extract as Immunoregulator on Dental Pulp Mesenchymal Stem Cells via Paracrine Pathway |
| title_full_unstemmed | Effect of Robusta Coffee Bean Extract as Immunoregulator on Dental Pulp Mesenchymal Stem Cells via Paracrine Pathway |
| title_short | Effect of Robusta Coffee Bean Extract as Immunoregulator on Dental Pulp Mesenchymal Stem Cells via Paracrine Pathway |
| title_sort | effect of robusta coffee bean extract as immunoregulator on dental pulp mesenchymal stem cells via paracrine pathway |
| topic | Dental Pulp Mesenchymal Stem Cells Secretome Herbal |
| url | https://revista.uepb.edu.br/PBOCI/article/view/4290 |
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