The exocyst complex controls multiple events in the pathway of regulated exocytosis
Eukaryotic cells depend on exocytosis to direct intracellularly synthesized material toward the extracellular space or the plasma membrane, so exocytosis constitutes a basic function for cellular homeostasis and communication between cells. The secretory pathway includes biogenesis of secretory gran...
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eLife Sciences Publications Ltd
2024-11-01
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| Online Access: | https://elifesciences.org/articles/92404 |
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| author | Sofía Suárez Freire Sebastián Perez-Pandolfo Sabrina Micaela Fresco Julián Valinoti Eleonora Sorianello Pablo Wappner Mariana Melani |
| author_facet | Sofía Suárez Freire Sebastián Perez-Pandolfo Sabrina Micaela Fresco Julián Valinoti Eleonora Sorianello Pablo Wappner Mariana Melani |
| author_sort | Sofía Suárez Freire |
| collection | DOAJ |
| description | Eukaryotic cells depend on exocytosis to direct intracellularly synthesized material toward the extracellular space or the plasma membrane, so exocytosis constitutes a basic function for cellular homeostasis and communication between cells. The secretory pathway includes biogenesis of secretory granules (SGs), their maturation and fusion with the plasma membrane (exocytosis), resulting in release of SG content to the extracellular space. The larval salivary gland of Drosophila melanogaster is an excellent model for studying exocytosis. This gland synthesizes mucins that are packaged in SGs that sprout from the trans-Golgi network and then undergo a maturation process that involves homotypic fusion, condensation, and acidification. Finally, mature SGs are directed to the apical domain of the plasma membrane with which they fuse, releasing their content into the gland lumen. The exocyst is a hetero-octameric complex that participates in tethering of vesicles to the plasma membrane during constitutive exocytosis. By precise temperature-dependent gradual activation of the Gal4-UAS expression system, we have induced different levels of silencing of exocyst complex subunits, and identified three temporarily distinctive steps of the regulated exocytic pathway where the exocyst is critically required: SG biogenesis, SG maturation, and SG exocytosis. Our results shed light on previously unidentified functions of the exocyst along the exocytic pathway. We propose that the exocyst acts as a general tethering factor in various steps of this cellular process. |
| format | Article |
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| institution | OA Journals |
| issn | 2050-084X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | eLife Sciences Publications Ltd |
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| spelling | doaj-art-28b85813e0cf49b8aca342bc44ca576c2025-08-20T01:53:34ZengeLife Sciences Publications LtdeLife2050-084X2024-11-011210.7554/eLife.92404The exocyst complex controls multiple events in the pathway of regulated exocytosisSofía Suárez Freire0https://orcid.org/0009-0003-2908-3110Sebastián Perez-Pandolfo1Sabrina Micaela Fresco2Julián Valinoti3Eleonora Sorianello4Pablo Wappner5https://orcid.org/0000-0003-1517-0742Mariana Melani6https://orcid.org/0000-0002-9491-932XFundación Instituto Leloir, Buenos Aires, Argentina; Consejo Nacional De Investigaciones Científicas Y Técnicas (CONICET), Buenos Aries, ArgentinaFundación Instituto Leloir, Buenos Aires, Argentina; Consejo Nacional De Investigaciones Científicas Y Técnicas (CONICET), Buenos Aries, ArgentinaFundación Instituto Leloir, Buenos Aires, ArgentinaFundación Instituto Leloir, Buenos Aires, ArgentinaConsejo Nacional De Investigaciones Científicas Y Técnicas (CONICET), Buenos Aries, Argentina; Laboratorio de Regulación Hipofisaria, Instituto de Biología y Medicina Experimental (IBYME-CONICET), Buenos Aires, ArgentinaFundación Instituto Leloir, Buenos Aires, Argentina; Consejo Nacional De Investigaciones Científicas Y Técnicas (CONICET), Buenos Aries, Argentina; Departamento De Fisiología, Biología Molecular Y Celular, Facultad De Ciencias Exactas Y Naturales, Universidad De Buenos Aires, Buenos Aires, ArgentinaFundación Instituto Leloir, Buenos Aires, Argentina; Consejo Nacional De Investigaciones Científicas Y Técnicas (CONICET), Buenos Aries, Argentina; Departamento De Fisiología, Biología Molecular Y Celular, Facultad De Ciencias Exactas Y Naturales, Universidad De Buenos Aires, Buenos Aires, ArgentinaEukaryotic cells depend on exocytosis to direct intracellularly synthesized material toward the extracellular space or the plasma membrane, so exocytosis constitutes a basic function for cellular homeostasis and communication between cells. The secretory pathway includes biogenesis of secretory granules (SGs), their maturation and fusion with the plasma membrane (exocytosis), resulting in release of SG content to the extracellular space. The larval salivary gland of Drosophila melanogaster is an excellent model for studying exocytosis. This gland synthesizes mucins that are packaged in SGs that sprout from the trans-Golgi network and then undergo a maturation process that involves homotypic fusion, condensation, and acidification. Finally, mature SGs are directed to the apical domain of the plasma membrane with which they fuse, releasing their content into the gland lumen. The exocyst is a hetero-octameric complex that participates in tethering of vesicles to the plasma membrane during constitutive exocytosis. By precise temperature-dependent gradual activation of the Gal4-UAS expression system, we have induced different levels of silencing of exocyst complex subunits, and identified three temporarily distinctive steps of the regulated exocytic pathway where the exocyst is critically required: SG biogenesis, SG maturation, and SG exocytosis. Our results shed light on previously unidentified functions of the exocyst along the exocytic pathway. We propose that the exocyst acts as a general tethering factor in various steps of this cellular process.https://elifesciences.org/articles/92404exocytosisexocyst complextethering complexsecretory granuleDrosophila melanogaster |
| spellingShingle | Sofía Suárez Freire Sebastián Perez-Pandolfo Sabrina Micaela Fresco Julián Valinoti Eleonora Sorianello Pablo Wappner Mariana Melani The exocyst complex controls multiple events in the pathway of regulated exocytosis eLife exocytosis exocyst complex tethering complex secretory granule Drosophila melanogaster |
| title | The exocyst complex controls multiple events in the pathway of regulated exocytosis |
| title_full | The exocyst complex controls multiple events in the pathway of regulated exocytosis |
| title_fullStr | The exocyst complex controls multiple events in the pathway of regulated exocytosis |
| title_full_unstemmed | The exocyst complex controls multiple events in the pathway of regulated exocytosis |
| title_short | The exocyst complex controls multiple events in the pathway of regulated exocytosis |
| title_sort | exocyst complex controls multiple events in the pathway of regulated exocytosis |
| topic | exocytosis exocyst complex tethering complex secretory granule Drosophila melanogaster |
| url | https://elifesciences.org/articles/92404 |
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