The study of antiproliferative and apoptosis-promoting effect of cinobufotalin on kidney cancer cells 786-O
Objective To study the antiproliferative and apoptosis-promoting effect of cinobufotalin on kidney cancer cells 786-O and its mechanism. Methods In vitro cultured kidney cancer cells 786-O were divided into control group,1 mg/mL cinobufotalin group, 10 mg/mL cinobufotalin group and 100 mg/mL cinobuf...
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| Main Authors: | , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Journal of Clinical Nephrology
2019-01-01
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| Series: | Linchuang shenzangbing zazhi |
| Subjects: | |
| Online Access: | http://www.lcszb.com/thesisDetails?columnId=57909530&Fpath=home&index=0 |
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| Summary: | Objective To study the antiproliferative and apoptosis-promoting effect of cinobufotalin on kidney cancer cells 786-O and its mechanism. Methods In vitro cultured kidney cancer cells 786-O were divided into control group,1 mg/mL cinobufotalin group, 10 mg/mL cinobufotalin group and 100 mg/mL cinobufotalin group.Cell proliferation inhibition rate was calculated, cell apoptosis rate wasmeasured by flow cytometry, nucleus apoptosis were detected by Hoechst-PI nuclear staining, and expressions of the protein Survivin were detected by western blot method. Results Cell proliferation inhibition rate increased with cinobufotalin concentrations, and exhibited time-dependent. Flow cytometry and Hoechst-PI nuclear staining test results showed that kidney cancer cells 786-O apoptosis rate increased in a dose-dependent manner as cinobufotalin concentration increased. Western blot showed that cinobufotalin inhibited the expression of survivin. Conclusions Cinobufotalin inhibits kidney cancer cells 786-O’s proliferation and promotes cancer cell apoptosis by down-regulating the expression of Survivin. |
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| ISSN: | 1671-2390 |