Oxidative Coupling Reaction for Micro Trace Analysis of Mebendazol Residual with p-bromoaniline in Presence of n- bromosuccinimide
Rapid, reproducible and accurate method has been developed for the assay for of mebendazol (MBZ) residual assay. The method is based on alkaline hydrolysis of MBZ with sodium hydroxide then oxidation with N-bromosuccinimide (NBS) followed by coupling with 4-Bromoaniline (4-BA) to yield a highly colo...
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University of Baghdad, College of Science for Women
2020-07-01
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| Series: | مجلة بغداد للعلوم |
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| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3793 |
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| author | Takleef Sallal sumayha Abbas |
| author_facet | Takleef Sallal sumayha Abbas |
| author_sort | Takleef Sallal |
| collection | DOAJ |
| description | Rapid, reproducible and accurate method has been developed for the assay for of mebendazol (MBZ) residual assay. The method is based on alkaline hydrolysis of MBZ with sodium hydroxide then oxidation with N-bromosuccinimide (NBS) followed by coupling with 4-Bromoaniline (4-BA) to yield a highly colored product absorbed at maximum 434 nm. Regression analysis of linearity range was found (0.6-2.8) µg.ml-1. The optimum conditions that affect the oxidation were studied. The developed method was found to be precise with mean value of relative standard deviation (1.153- 1.303) and accurate with relative error (-0.5940-1.7821) .The calculated molar absorptivity and sandal sensitivity values of (29825 L.mol-1.cm-1), 0.0099 µg.cm-2 respectively. The limit of detection and limit of quantitation were of 0.04696, 0.156548 µg.ml-1 respectively .The suggested method showed good recovery with a mean value of 100.77% for analysis of dosage forms. |
| format | Article |
| id | doaj-art-28a4b85d31fc4a94b18c6d467b17fbe7 |
| institution | Kabale University |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2020-07-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-28a4b85d31fc4a94b18c6d467b17fbe72025-08-20T03:33:10ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862020-07-0117310.21123/bsj.2020.17.3.0780Oxidative Coupling Reaction for Micro Trace Analysis of Mebendazol Residual with p-bromoaniline in Presence of n- bromosuccinimideTakleef Sallal0sumayha Abbas1Ministry of Education, Baghdad, Iraquniversity of baghdad .college ibn Al-HaithamRapid, reproducible and accurate method has been developed for the assay for of mebendazol (MBZ) residual assay. The method is based on alkaline hydrolysis of MBZ with sodium hydroxide then oxidation with N-bromosuccinimide (NBS) followed by coupling with 4-Bromoaniline (4-BA) to yield a highly colored product absorbed at maximum 434 nm. Regression analysis of linearity range was found (0.6-2.8) µg.ml-1. The optimum conditions that affect the oxidation were studied. The developed method was found to be precise with mean value of relative standard deviation (1.153- 1.303) and accurate with relative error (-0.5940-1.7821) .The calculated molar absorptivity and sandal sensitivity values of (29825 L.mol-1.cm-1), 0.0099 µg.cm-2 respectively. The limit of detection and limit of quantitation were of 0.04696, 0.156548 µg.ml-1 respectively .The suggested method showed good recovery with a mean value of 100.77% for analysis of dosage forms.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3793Mebendazol, N- bromosuccinimide, Oxidation coupling, Spectrophotometry. |
| spellingShingle | Takleef Sallal sumayha Abbas Oxidative Coupling Reaction for Micro Trace Analysis of Mebendazol Residual with p-bromoaniline in Presence of n- bromosuccinimide مجلة بغداد للعلوم Mebendazol, N- bromosuccinimide, Oxidation coupling, Spectrophotometry. |
| title | Oxidative Coupling Reaction for Micro Trace Analysis of Mebendazol Residual with p-bromoaniline in Presence of n- bromosuccinimide |
| title_full | Oxidative Coupling Reaction for Micro Trace Analysis of Mebendazol Residual with p-bromoaniline in Presence of n- bromosuccinimide |
| title_fullStr | Oxidative Coupling Reaction for Micro Trace Analysis of Mebendazol Residual with p-bromoaniline in Presence of n- bromosuccinimide |
| title_full_unstemmed | Oxidative Coupling Reaction for Micro Trace Analysis of Mebendazol Residual with p-bromoaniline in Presence of n- bromosuccinimide |
| title_short | Oxidative Coupling Reaction for Micro Trace Analysis of Mebendazol Residual with p-bromoaniline in Presence of n- bromosuccinimide |
| title_sort | oxidative coupling reaction for micro trace analysis of mebendazol residual with p bromoaniline in presence of n bromosuccinimide |
| topic | Mebendazol, N- bromosuccinimide, Oxidation coupling, Spectrophotometry. |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/3793 |
| work_keys_str_mv | AT takleefsallal oxidativecouplingreactionformicrotraceanalysisofmebendazolresidualwithpbromoanilineinpresenceofnbromosuccinimide AT sumayhaabbas oxidativecouplingreactionformicrotraceanalysisofmebendazolresidualwithpbromoanilineinpresenceofnbromosuccinimide |