Sperm Separation and Selection Techniques to Mitigate Sperm DNA Damage
Semen preparation and sperm selection techniques exploit the morphological and physiological characteristics of sperm function, including motility, morphology, density, and maturity, as reflected by their cell-surface charge and the expression of hyaluronan receptors. The various methods employed ha...
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MDPI AG
2025-02-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/15/2/302 |
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| author | Steven Fleming David Morroll Martine Nijs |
| author_facet | Steven Fleming David Morroll Martine Nijs |
| author_sort | Steven Fleming |
| collection | DOAJ |
| description | Semen preparation and sperm selection techniques exploit the morphological and physiological characteristics of sperm function, including motility, morphology, density, and maturity, as reflected by their cell-surface charge and the expression of hyaluronan receptors. The various methods employed have a common purpose of mimicing sperm selection within the female reproductive tract and, thereby, increasing the likelihood that oocytes will be fertilised by spermatozoa with intact nuclear DNA and a normal genome. Indeed, the paternal genome is relevant to embryonic genome activation and blastocyst development, and has a fundamental impact upon successful implantation, ongoing pregnancy and live birth. The clinical use of both well-established and some more recently developed techniques is discussed in this comparative clinical review of sperm separation from seminal plasma and selection for insemination. |
| format | Article |
| id | doaj-art-2897afae70e44db8a6bb83cf9676a927 |
| institution | DOAJ |
| issn | 2075-1729 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj-art-2897afae70e44db8a6bb83cf9676a9272025-08-20T03:12:00ZengMDPI AGLife2075-17292025-02-0115230210.3390/life15020302Sperm Separation and Selection Techniques to Mitigate Sperm DNA DamageSteven Fleming0David Morroll1Martine Nijs2Discipline of Anatomy & Histology, School of Medical Sciences, University of Sydney, Sydney, NSW 2000, AustraliaCooperSurgical, 2750 Ballerup, DenmarkCooperSurgical, 2750 Ballerup, DenmarkSemen preparation and sperm selection techniques exploit the morphological and physiological characteristics of sperm function, including motility, morphology, density, and maturity, as reflected by their cell-surface charge and the expression of hyaluronan receptors. The various methods employed have a common purpose of mimicing sperm selection within the female reproductive tract and, thereby, increasing the likelihood that oocytes will be fertilised by spermatozoa with intact nuclear DNA and a normal genome. Indeed, the paternal genome is relevant to embryonic genome activation and blastocyst development, and has a fundamental impact upon successful implantation, ongoing pregnancy and live birth. The clinical use of both well-established and some more recently developed techniques is discussed in this comparative clinical review of sperm separation from seminal plasma and selection for insemination.https://www.mdpi.com/2075-1729/15/2/302semen preparationsperm DNA fragmentationsperm selectionsperm separation |
| spellingShingle | Steven Fleming David Morroll Martine Nijs Sperm Separation and Selection Techniques to Mitigate Sperm DNA Damage Life semen preparation sperm DNA fragmentation sperm selection sperm separation |
| title | Sperm Separation and Selection Techniques to Mitigate Sperm DNA Damage |
| title_full | Sperm Separation and Selection Techniques to Mitigate Sperm DNA Damage |
| title_fullStr | Sperm Separation and Selection Techniques to Mitigate Sperm DNA Damage |
| title_full_unstemmed | Sperm Separation and Selection Techniques to Mitigate Sperm DNA Damage |
| title_short | Sperm Separation and Selection Techniques to Mitigate Sperm DNA Damage |
| title_sort | sperm separation and selection techniques to mitigate sperm dna damage |
| topic | semen preparation sperm DNA fragmentation sperm selection sperm separation |
| url | https://www.mdpi.com/2075-1729/15/2/302 |
| work_keys_str_mv | AT stevenfleming spermseparationandselectiontechniquestomitigatespermdnadamage AT davidmorroll spermseparationandselectiontechniquestomitigatespermdnadamage AT martinenijs spermseparationandselectiontechniquestomitigatespermdnadamage |