Investigation of the effect of human intestinal microbiota bacteria on the bioremediation of commonly used pesticides by liquid chromatography
Pesticides are defined as chemical substances used to protect a variety of plants from parasite infestation. Due to their chemical structure, they have a long half-life and the capacity to form residues, and therefore many fruits can contain pesticide residues. Pesticide exposure may occur via inhal...
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Elsevier
2024-12-01
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| Series: | The Microbe |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950194624001377 |
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| author | Ahmet Aktaş İlker Aras Göçer Yaşar Naki̇poğlu |
| author_facet | Ahmet Aktaş İlker Aras Göçer Yaşar Naki̇poğlu |
| author_sort | Ahmet Aktaş |
| collection | DOAJ |
| description | Pesticides are defined as chemical substances used to protect a variety of plants from parasite infestation. Due to their chemical structure, they have a long half-life and the capacity to form residues, and therefore many fruits can contain pesticide residues. Pesticide exposure may occur via inhalation, ingestion, or direct contact. The European Commission's directive restricts the use of pesticides and sets a residue limit of 0.1 μg/L. The aim of this study is to determine the impact of bacteria in the human gut microbiota on the biodegradation of commonly used pesticides. This study investigated the effects of five bacterial strains, namely Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae (K. pneumoniae), and Enterococcus faecalis (E. faecalis), isolated from neonatal rectal swab samples and, blood samples on the degradation of pesticides using liquid chromatography and mass spectrometry. (LC-MS). All bacteria resulted in significant pesticide degradation (p < 0.05). E. faecalis caused significantly more degradation than K. pneumoniae (p = 0.043). No significant difference was observed between the degradation rates of pesticides and other bacteria (p > 0.05). The results demonstrate that bacteria can be used to clean water and soil contaminated with pesticides. Therefore, we do not have information about possible mutations in DNA. This is the biggest limitation of this study. Therefore, our study data needs to be supported with study data including more strains and examining bacterial DNA with new generation sequencing methods. |
| format | Article |
| id | doaj-art-289197d84d0143d5a7e78d73cee6aa19 |
| institution | OA Journals |
| issn | 2950-1946 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | The Microbe |
| spelling | doaj-art-289197d84d0143d5a7e78d73cee6aa192025-08-20T01:57:55ZengElsevierThe Microbe2950-19462024-12-01510017010.1016/j.microb.2024.100170Investigation of the effect of human intestinal microbiota bacteria on the bioremediation of commonly used pesticides by liquid chromatographyAhmet Aktaş0İlker Aras Göçer1Yaşar Naki̇poğlu2Istanbul Provincial Health Directorate, Istanbul Public Health Laboratory No. 2, Türkiye; Department of Medical Microbiology, Istanbul Medical Faculty, Istanbul University, Türkiye; Corresponding author at: Istanbul Provincial Health Directorate, Istanbul Public Health Laboratory No. 2, Türkiye.Institute of Forensic Science and Legal Medicine, Istanbul University-Cerrahpaşa, TürkiyeDepartment of Medical Microbiology, Istanbul Medical Faculty, Istanbul University, TürkiyePesticides are defined as chemical substances used to protect a variety of plants from parasite infestation. Due to their chemical structure, they have a long half-life and the capacity to form residues, and therefore many fruits can contain pesticide residues. Pesticide exposure may occur via inhalation, ingestion, or direct contact. The European Commission's directive restricts the use of pesticides and sets a residue limit of 0.1 μg/L. The aim of this study is to determine the impact of bacteria in the human gut microbiota on the biodegradation of commonly used pesticides. This study investigated the effects of five bacterial strains, namely Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae (K. pneumoniae), and Enterococcus faecalis (E. faecalis), isolated from neonatal rectal swab samples and, blood samples on the degradation of pesticides using liquid chromatography and mass spectrometry. (LC-MS). All bacteria resulted in significant pesticide degradation (p < 0.05). E. faecalis caused significantly more degradation than K. pneumoniae (p = 0.043). No significant difference was observed between the degradation rates of pesticides and other bacteria (p > 0.05). The results demonstrate that bacteria can be used to clean water and soil contaminated with pesticides. Therefore, we do not have information about possible mutations in DNA. This is the biggest limitation of this study. Therefore, our study data needs to be supported with study data including more strains and examining bacterial DNA with new generation sequencing methods.http://www.sciencedirect.com/science/article/pii/S2950194624001377ThiabendazoleLiquid Chromatography-Mass SpectrometryLinuronDiuronWaterMicrobiota |
| spellingShingle | Ahmet Aktaş İlker Aras Göçer Yaşar Naki̇poğlu Investigation of the effect of human intestinal microbiota bacteria on the bioremediation of commonly used pesticides by liquid chromatography The Microbe Thiabendazole Liquid Chromatography-Mass Spectrometry Linuron Diuron Water Microbiota |
| title | Investigation of the effect of human intestinal microbiota bacteria on the bioremediation of commonly used pesticides by liquid chromatography |
| title_full | Investigation of the effect of human intestinal microbiota bacteria on the bioremediation of commonly used pesticides by liquid chromatography |
| title_fullStr | Investigation of the effect of human intestinal microbiota bacteria on the bioremediation of commonly used pesticides by liquid chromatography |
| title_full_unstemmed | Investigation of the effect of human intestinal microbiota bacteria on the bioremediation of commonly used pesticides by liquid chromatography |
| title_short | Investigation of the effect of human intestinal microbiota bacteria on the bioremediation of commonly used pesticides by liquid chromatography |
| title_sort | investigation of the effect of human intestinal microbiota bacteria on the bioremediation of commonly used pesticides by liquid chromatography |
| topic | Thiabendazole Liquid Chromatography-Mass Spectrometry Linuron Diuron Water Microbiota |
| url | http://www.sciencedirect.com/science/article/pii/S2950194624001377 |
| work_keys_str_mv | AT ahmetaktas investigationoftheeffectofhumanintestinalmicrobiotabacteriaonthebioremediationofcommonlyusedpesticidesbyliquidchromatography AT ilkerarasgocer investigationoftheeffectofhumanintestinalmicrobiotabacteriaonthebioremediationofcommonlyusedpesticidesbyliquidchromatography AT yasarnakipoglu investigationoftheeffectofhumanintestinalmicrobiotabacteriaonthebioremediationofcommonlyusedpesticidesbyliquidchromatography |