Towards differentiation of Panax vietnamensis and Panax vietnamensis var. fuscidiscus by UPLC-QTOF-MS
Vietnamese ginseng (Panax vietnamensis Ha et Grusvh., Araliaceae - PV) is a high-value medicinal plant of Vietnam. Recently, a new variety of this plant P. vietnamensis var fuscidiscus (PVF), so-called Lai Chau ginseng, was discovered and circulated on the Vietnam market along with PV. PV and PVF rh...
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HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE
2024-04-01
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| Series: | Ho Chi Minh City Open University Journal of Science - Engineering and Technology |
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| Online Access: | https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/3047 |
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| author | Long Huynh Kim Vu Duc Minh Nguyen Long Ke Phan Yen Trang Hai Nguyen Huy Truong Nguyen |
| author_facet | Long Huynh Kim Vu Duc Minh Nguyen Long Ke Phan Yen Trang Hai Nguyen Huy Truong Nguyen |
| author_sort | Long Huynh Kim Vu |
| collection | DOAJ |
| description | Vietnamese ginseng (Panax vietnamensis Ha et Grusvh., Araliaceae - PV) is a high-value medicinal plant of Vietnam. Recently, a new variety of this plant P. vietnamensis var fuscidiscus (PVF), so-called Lai Chau ginseng, was discovered and circulated on the Vietnam market along with PV. PV and PVF rhizomes and roots are preliminarily reported to possess similar saponin composition. However, PV is much more expensive than PVF due to the higher regard of the consumer, which consequently leads to the inattentive misuse or intentional adulteration of PV by PVF. In this study, a UPLC-ToF-MS method was developed to distinguish PV and PVF based on their saponin profiles. The UPLC qualitative result showed that the saponin constituent of PVF is more complex than that of PV. The significant differences in the chromatogram of PV and PVF extracts are the peaks eluted from 32 - 48 min. Vina-ginsenoside R2 (9), chikusetsusaponin L8 (10), and notoginsenoside R4 (12) are characterized for PVF, while notoginsenoside Fa (13), quinquenoside R1 (16), and pseudoginsenoside Rs1 (19) are specialized for PV. The preliminary quantitative determination result showed that the content of majonoside R2, ginsenoside Rg1, ginsenoside Rb1, and ginsenoside Rd in PV is higher than in PVF. In contrast, the content of majonoside R1 and vina-ginsenoside R2 in PVF is significantly higher than in PV. The developed method could be used as an effective method to distinguish PV and PVF. |
| format | Article |
| id | doaj-art-288d24378bcb4d62bf1dd15b75c12bf1 |
| institution | Kabale University |
| issn | 2734-9330 2734-9608 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | HO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCE |
| record_format | Article |
| series | Ho Chi Minh City Open University Journal of Science - Engineering and Technology |
| spelling | doaj-art-288d24378bcb4d62bf1dd15b75c12bf12024-11-15T05:39:57ZengHO CHI MINH CITY OPEN UNIVERSITY JOURNAL OF SCIENCEHo Chi Minh City Open University Journal of Science - Engineering and Technology2734-93302734-96082024-04-01142192710.46223/HCMCOUJS.tech.en.14.2.3047.20242141Towards differentiation of Panax vietnamensis and Panax vietnamensis var. fuscidiscus by UPLC-QTOF-MSLong Huynh Kim Vu0Duc Minh Nguyen1Long Ke Phan2Yen Trang Hai Nguyen3Huy Truong Nguyen4Ton Duc Thang University, Ho Chi Minh CityTon Duc Thang University, Ho Chi Minh CityVietnam National Museum of Nature, Vietnam Academy of Science and Technology, HanoiTon Duc Thang University, Ho Chi Minh CityTon Duc Thang University, Ho Chi Minh CityVietnamese ginseng (Panax vietnamensis Ha et Grusvh., Araliaceae - PV) is a high-value medicinal plant of Vietnam. Recently, a new variety of this plant P. vietnamensis var fuscidiscus (PVF), so-called Lai Chau ginseng, was discovered and circulated on the Vietnam market along with PV. PV and PVF rhizomes and roots are preliminarily reported to possess similar saponin composition. However, PV is much more expensive than PVF due to the higher regard of the consumer, which consequently leads to the inattentive misuse or intentional adulteration of PV by PVF. In this study, a UPLC-ToF-MS method was developed to distinguish PV and PVF based on their saponin profiles. The UPLC qualitative result showed that the saponin constituent of PVF is more complex than that of PV. The significant differences in the chromatogram of PV and PVF extracts are the peaks eluted from 32 - 48 min. Vina-ginsenoside R2 (9), chikusetsusaponin L8 (10), and notoginsenoside R4 (12) are characterized for PVF, while notoginsenoside Fa (13), quinquenoside R1 (16), and pseudoginsenoside Rs1 (19) are specialized for PV. The preliminary quantitative determination result showed that the content of majonoside R2, ginsenoside Rg1, ginsenoside Rb1, and ginsenoside Rd in PV is higher than in PVF. In contrast, the content of majonoside R1 and vina-ginsenoside R2 in PVF is significantly higher than in PV. The developed method could be used as an effective method to distinguish PV and PVF.https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/3047lai chau ginsengpanax vietnamensispanax vietnamensis var. fuscidiscusuplc-tof-msvietnamese ginseng |
| spellingShingle | Long Huynh Kim Vu Duc Minh Nguyen Long Ke Phan Yen Trang Hai Nguyen Huy Truong Nguyen Towards differentiation of Panax vietnamensis and Panax vietnamensis var. fuscidiscus by UPLC-QTOF-MS Ho Chi Minh City Open University Journal of Science - Engineering and Technology lai chau ginseng panax vietnamensis panax vietnamensis var. fuscidiscus uplc-tof-ms vietnamese ginseng |
| title | Towards differentiation of Panax vietnamensis and Panax vietnamensis var. fuscidiscus by UPLC-QTOF-MS |
| title_full | Towards differentiation of Panax vietnamensis and Panax vietnamensis var. fuscidiscus by UPLC-QTOF-MS |
| title_fullStr | Towards differentiation of Panax vietnamensis and Panax vietnamensis var. fuscidiscus by UPLC-QTOF-MS |
| title_full_unstemmed | Towards differentiation of Panax vietnamensis and Panax vietnamensis var. fuscidiscus by UPLC-QTOF-MS |
| title_short | Towards differentiation of Panax vietnamensis and Panax vietnamensis var. fuscidiscus by UPLC-QTOF-MS |
| title_sort | towards differentiation of panax vietnamensis and panax vietnamensis var fuscidiscus by uplc qtof ms |
| topic | lai chau ginseng panax vietnamensis panax vietnamensis var. fuscidiscus uplc-tof-ms vietnamese ginseng |
| url | https://journalofscience.ou.edu.vn/index.php/tech-en/article/view/3047 |
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