Pharmacodynamics of huaier aqueous extract against papillary thyroid carcinoma in vivo and in vitro

Papillary thyroid carcinoma (PTC) is the main type of malignant tumor of the thyroid gland in adults. Huaier, a traditional Chinese medicinal agent, has demonstrated antitumor efficacy across diverse malignant neoplasms. However, the specific impact of huaier in PTC remains insufficiently elucidated...

Full description

Saved in:
Bibliographic Details
Main Authors: Fa-Zhan Xu MS, Lu-Lu Zheng MS, Jia-Bo Qin MS, Yi-Xuan Li MS, Ke-Hao Chen MS, Ling-Hui Dai MS, Qiao-Ling Zhu MS, Jian-Feng Sang MD
Format: Article
Language:English
Published: SAGE Publishing 2025-05-01
Series:Integrative Cancer Therapies
Online Access:https://doi.org/10.1177/15347354251339073
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849313416907325440
author Fa-Zhan Xu MS
Lu-Lu Zheng MS
Jia-Bo Qin MS
Yi-Xuan Li MS
Ke-Hao Chen MS
Ling-Hui Dai MS
Qiao-Ling Zhu MS
Jian-Feng Sang MD
author_facet Fa-Zhan Xu MS
Lu-Lu Zheng MS
Jia-Bo Qin MS
Yi-Xuan Li MS
Ke-Hao Chen MS
Ling-Hui Dai MS
Qiao-Ling Zhu MS
Jian-Feng Sang MD
author_sort Fa-Zhan Xu MS
collection DOAJ
description Papillary thyroid carcinoma (PTC) is the main type of malignant tumor of the thyroid gland in adults. Huaier, a traditional Chinese medicinal agent, has demonstrated antitumor efficacy across diverse malignant neoplasms. However, the specific impact of huaier in PTC remains insufficiently elucidated. In this study, we explored the function of huaier in PTC. We treated a PTC cell line (TPC-1) with varying concentration gradients of huaier and we observed that huaier effectively inhibited the proliferation of TPC-1 cells with an IC50 of 2.538 mg/ml. Huaier also modulated the cell cycle of the TPC-1 cell line, and induced apoptosis in TPC-1. Huaier treatment inhibited migration and invasion of TPC-1 cells. Further studies showed that in TPC-1 cells, huaier treatment inhibited the expression of the MAPK and HSP27/STAT3/AKT signaling pathway. We subsequently demonstrated in a nude mouse model that huaier treatment inhibited the progression of PTC tumors. In summary, our data suggest that huaier can inhibit the proliferation, migration, and invasion of PTC cells in vitro and the progression of PTC tumors in vivo, and that this effect may be related to the MAPK and HSP27/STAT3/AKT signaling pathway; therefore, huaier extract is expected to be a new direction for the treatment of PTC.
format Article
id doaj-art-287d8565dbc24528b67a94a46bf7b30b
institution Kabale University
issn 1552-695X
language English
publishDate 2025-05-01
publisher SAGE Publishing
record_format Article
series Integrative Cancer Therapies
spelling doaj-art-287d8565dbc24528b67a94a46bf7b30b2025-08-20T03:52:47ZengSAGE PublishingIntegrative Cancer Therapies1552-695X2025-05-012410.1177/15347354251339073Pharmacodynamics of huaier aqueous extract against papillary thyroid carcinoma in vivo and in vitroFa-Zhan Xu MS0Lu-Lu Zheng MS1Jia-Bo Qin MS2Yi-Xuan Li MS3Ke-Hao Chen MS4Ling-Hui Dai MS5Qiao-Ling Zhu MS6Jian-Feng Sang MD7Division of Thyroid Surgery, Department of General Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, ChinaDivision of Thyroid Surgery, Department of General Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, ChinaDivision of Thyroid Surgery, Department of General Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, ChinaMedical School of Nanjing University, Nanjing, ChinaDivision of Thyroid Surgery, Department of General Surgery, Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University, Nanjing, ChinaMedical School of Nanjing University, Nanjing, ChinaDepartment of Pharmacy, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, ChinaDivision of Thyroid Surgery, Department of General Surgery, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, ChinaPapillary thyroid carcinoma (PTC) is the main type of malignant tumor of the thyroid gland in adults. Huaier, a traditional Chinese medicinal agent, has demonstrated antitumor efficacy across diverse malignant neoplasms. However, the specific impact of huaier in PTC remains insufficiently elucidated. In this study, we explored the function of huaier in PTC. We treated a PTC cell line (TPC-1) with varying concentration gradients of huaier and we observed that huaier effectively inhibited the proliferation of TPC-1 cells with an IC50 of 2.538 mg/ml. Huaier also modulated the cell cycle of the TPC-1 cell line, and induced apoptosis in TPC-1. Huaier treatment inhibited migration and invasion of TPC-1 cells. Further studies showed that in TPC-1 cells, huaier treatment inhibited the expression of the MAPK and HSP27/STAT3/AKT signaling pathway. We subsequently demonstrated in a nude mouse model that huaier treatment inhibited the progression of PTC tumors. In summary, our data suggest that huaier can inhibit the proliferation, migration, and invasion of PTC cells in vitro and the progression of PTC tumors in vivo, and that this effect may be related to the MAPK and HSP27/STAT3/AKT signaling pathway; therefore, huaier extract is expected to be a new direction for the treatment of PTC.https://doi.org/10.1177/15347354251339073
spellingShingle Fa-Zhan Xu MS
Lu-Lu Zheng MS
Jia-Bo Qin MS
Yi-Xuan Li MS
Ke-Hao Chen MS
Ling-Hui Dai MS
Qiao-Ling Zhu MS
Jian-Feng Sang MD
Pharmacodynamics of huaier aqueous extract against papillary thyroid carcinoma in vivo and in vitro
Integrative Cancer Therapies
title Pharmacodynamics of huaier aqueous extract against papillary thyroid carcinoma in vivo and in vitro
title_full Pharmacodynamics of huaier aqueous extract against papillary thyroid carcinoma in vivo and in vitro
title_fullStr Pharmacodynamics of huaier aqueous extract against papillary thyroid carcinoma in vivo and in vitro
title_full_unstemmed Pharmacodynamics of huaier aqueous extract against papillary thyroid carcinoma in vivo and in vitro
title_short Pharmacodynamics of huaier aqueous extract against papillary thyroid carcinoma in vivo and in vitro
title_sort pharmacodynamics of huaier aqueous extract against papillary thyroid carcinoma in vivo and in vitro
url https://doi.org/10.1177/15347354251339073
work_keys_str_mv AT fazhanxums pharmacodynamicsofhuaieraqueousextractagainstpapillarythyroidcarcinomainvivoandinvitro
AT luluzhengms pharmacodynamicsofhuaieraqueousextractagainstpapillarythyroidcarcinomainvivoandinvitro
AT jiaboqinms pharmacodynamicsofhuaieraqueousextractagainstpapillarythyroidcarcinomainvivoandinvitro
AT yixuanlims pharmacodynamicsofhuaieraqueousextractagainstpapillarythyroidcarcinomainvivoandinvitro
AT kehaochenms pharmacodynamicsofhuaieraqueousextractagainstpapillarythyroidcarcinomainvivoandinvitro
AT linghuidaims pharmacodynamicsofhuaieraqueousextractagainstpapillarythyroidcarcinomainvivoandinvitro
AT qiaolingzhums pharmacodynamicsofhuaieraqueousextractagainstpapillarythyroidcarcinomainvivoandinvitro
AT jianfengsangmd pharmacodynamicsofhuaieraqueousextractagainstpapillarythyroidcarcinomainvivoandinvitro